(Circular) (Six-base) MAPSORT of: pFN21AE6363.seq Check: 135 from: 1 to: 5977 pFN21AE6363 5977 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2057 3320 1937 Size: 120 1263 4594 Fragments arranged by size: 4594 1263 120 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 2664 2786 2843 2996 3046 4040 5424 Size: 818 214 122 57 153 50 994 1384 Cuts at: 5424 5692 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 994 818 268 214 153 122 57 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2991 4876 5853 1203 Size: 1788 1885 977 1327 Fragments arranged by size: 1885 1788 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 3038 1166 Size: 1872 4105 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2978 3010 2978 Size: 32 5945 AlwNI CAG_nnn'CTG Cuts at: 3044 3720 5292 3044 Size: 676 1572 3729 Fragments arranged by size: 3729 1572 676 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2248 2273 2386 3077 3286 3512 3664 5773 Size: 755 25 113 691 209 226 152 2109 Cuts at: 5773 1493 Size: 1697 Fragments arranged by size: 2109 1697 755 691 226 209 152 113 25 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3302 1870 Size: 73 1359 4545 Fragments arranged by size: 4545 1359 73 AvrII C'CTAG_G Cuts at: 3158 3158 Size: 5977 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3261 3294 3261 Size: 33 5944 BamHI G'GATC_C Cuts at: 2448 3171 3307 3674 2448 Size: 723 136 367 4751 Fragments arranged by size: 4751 723 367 136 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 3298 4753 618 Size: 325 217 627 285 1226 1455 1842 Fragments arranged by size: 1842 1455 1226 627 325 285 217 BanII G_rGCy'C Cuts at: 1391 3296 1391 Size: 1905 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2329 928 Size: 971 430 4576 Fragments arranged by size: 4576 971 430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 2875 1548 Size: 1327 4650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3178 5972 3178 Size: 2794 3183 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 4038 5194 1790 Size: 2248 1156 2573 Fragments arranged by size: 2573 2248 1156 BmgBI CAC'GTC Cuts at: 1318 2537 1318 Size: 1219 4758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4637 1239 Size: 3398 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2080 3123 4102 4967 5265 2080 Size: 1043 979 865 298 2792 Fragments arranged by size: 2792 1043 979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2437 3784 4166 4801 Size: 53 83 186 936 904 1347 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1451 1347 936 904 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3145 4410 4808 5082 5229 5640 3145 Size: 1265 398 274 147 411 3482 Fragments arranged by size: 3482 1265 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2857 2887 1178 Size: 30 165 30 1454 30 4268 Fragments arranged by size: 4268 1454 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2878 1186 Size: 1692 4285 BseYI C'CCAG_C Cuts at: 5180 5676 5180 Size: 496 5481 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 4038 4123 5194 2177 Size: 1861 85 1071 2960 Fragments arranged by size: 2960 1861 1071 85 BsmI GAATG_Cn' Cuts at: 2829 2945 3488 3581 2829 Size: 116 543 93 5225 Fragments arranged by size: 5225 543 116 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 3296 4038 4123 5194 1391 Size: 399 387 1119 742 85 1071 2174 Fragments arranged by size: 2174 1119 1071 742 399 387 85 BspEI T'CCGG_A Cuts at: 3145 5640 3145 Size: 2495 3482 BspHI T'CATG_A Cuts at: 2286 3866 5596 2286 Size: 1580 1730 2667 Fragments arranged by size: 2667 1730 1580 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2082 4472 4646 65 Size: 1183 834 2390 174 1396 Fragments arranged by size: 2390 1396 1183 834 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 2618 96 Size: 2522 3455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 3016 1184 Size: 1832 4145 BstYI r'GATC_y Cuts at: 1972 2448 3171 3178 3307 3674 4059 4076 5528 Size: 476 723 7 129 367 385 17 1452 Cuts at: 5528 5614 5626 5972 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 723 476 385 367 346 129 86 17 12 7 BstZ17I GTA'TAC Cuts at: 2058 2058 Size: 5977 BtgI C'CryG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 4123 Fragments arranged by size: 4123 939 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 3493 4287 4307 3493 Size: 794 20 5163 Fragments arranged by size: 5163 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2905 3424 4313 Size: 54 1425 50 275 18 1075 519 889 Cuts at: 4313 5942 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1075 889 519 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 2426 3907 1353 Size: 1062 11 1481 3423 Fragments arranged by size: 3423 1481 1062 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2664 2786 2843 2996 3046 4040 Size: 393 818 214 122 57 153 50 994 Cuts at: 4040 4637 5424 5692 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 994 818 787 597 393 268 214 153 122 57 50 EcoO109I rG'GnC_Cy Cuts at: 2953 3066 2953 Size: 113 5864 EcoRI G'AATT_C Cuts at: 2273 3286 3664 2273 Size: 1013 378 4586 Fragments arranged by size: 4586 1013 378 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3163 3195 4618 4650 4692 Size: 32 135 32 1591 32 1423 32 42 Cuts at: 4692 4724 1373 Size: 32 2626 Fragments arranged by size: 2626 1591 1423 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2049 3321 3567 669 Size: 988 281 111 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 111 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 2614 3576 2614 Size: 962 5015 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2180 5091 5275 1305 Size: 875 2911 184 2007 Fragments arranged by size: 2911 2007 875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2907 10 Size: 54 1425 343 1075 3080 Fragments arranged by size: 3080 1425 1075 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2550 3038 3935 4294 4453 518 Size: 648 1384 488 897 359 159 2042 Fragments arranged by size: 2042 1384 897 648 488 359 159 MspA1I CmG'CkG Cuts at: 1606 3393 4070 5218 5463 1606 Size: 1787 677 1148 245 2120 Fragments arranged by size: 2120 1787 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 4123 Fragments arranged by size: 4123 939 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2995 3335 4880 1132 Size: 97 1766 340 1545 2229 Fragments arranged by size: 2229 1766 1545 340 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 2991 4876 2991 Size: 1885 4092 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PfoI T'CCnGG_A Cuts at: 2264 2319 2982 2264 Size: 55 663 5259 Fragments arranged by size: 5259 663 55 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 2225 2399 3123 3547 2225 Size: 174 724 424 4655 Fragments arranged by size: 4655 724 424 174 PstI C_TGCA'G Cuts at: 830 3329 830 Size: 2499 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2415 2415 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2020 3325 4460 5141 5332 826 Size: 219 975 1305 1135 681 191 1471 Fragments arranged by size: 1471 1305 1135 975 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 3138 4081 4982 5244 820 Size: 197 926 152 1043 943 901 262 1553 Fragments arranged by size: 1553 1043 943 926 901 262 197 152 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 2110 152 Size: 1958 4019 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 3158 3416 513 Size: 552 939 363 791 258 3074 Fragments arranged by size: 3074 939 791 552 363 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2618 4223 5935 Size: 274 80 33 51 1443 641 1605 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1712 1605 1443 641 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2857 2889 5937 5969 2000 Size: 32 825 32 3048 32 2008 Fragments arranged by size: 3048 2008 825 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 2273 4106 2273 Size: 1833 4144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI