(Circular) (Six-base) MAPSORT of: pFN21AB5708.seq Check: 9405 from: 1 to: 6859 pFN21AB5708 6859 bp With 182 enzymes: SgfI * April 7, 2017 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3256 3256 Size: 6859 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4669 278 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4202 1937 Size: 2265 4594 Acc65I G'GTAC_C Cuts at: 4180 4180 Size: 6859 AclI AA'CG_TT Cuts at: 1563 4986 5359 1563 Size: 3423 373 3063 Fragments arranged by size: 3423 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2639 3109 3575 3762 3782 3888 4142 4922 Size: 1007 470 466 187 20 106 254 780 Cuts at: 4922 6306 6574 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1007 780 470 466 268 254 187 106 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 3339 5758 6735 1203 Size: 2136 2419 977 1327 Fragments arranged by size: 2419 2136 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 5588 5588 Size: 6859 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3123 3579 3869 1184 Size: 1939 456 290 4174 Fragments arranged by size: 4174 1939 456 290 AlwNI CAG_nnn'CTG Cuts at: 3147 3470 3499 4602 6174 3147 Size: 323 29 1103 1572 3832 Fragments arranged by size: 3832 1572 1103 323 29 ApaI G_GGCC'C Cuts at: 2174 2508 2174 Size: 334 6525 ApaLI G'TGCA_C Cuts at: 4916 6072 4916 Size: 1156 5703 ApoI r'AATT_y Cuts at: 1493 4168 4394 4546 6655 1493 Size: 2675 226 152 2109 1697 Fragments arranged by size: 2675 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5413 160 Size: 872 4381 1606 Fragments arranged by size: 4381 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2591 4184 1870 Size: 73 194 454 1593 4545 Fragments arranged by size: 4545 1593 454 194 73 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2179 2243 2270 4180 Size: 325 217 627 354 38 64 27 1910 Cuts at: 4180 5635 618 Size: 1455 1842 Fragments arranged by size: 1910 1842 1455 627 354 325 217 64 38 27 BanII G_rGCy'C Cuts at: 1391 2174 2301 2508 2750 2834 4150 4178 1391 Size: 783 127 207 242 84 1316 28 4072 Fragments arranged by size: 4072 1316 783 242 207 127 84 28 BbeI G_GCGC'C Cuts at: 2145 2183 2247 2274 2145 Size: 38 64 27 6730 Fragments arranged by size: 6730 64 38 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5888 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5050 5084 1903 Size: 34 3113 34 3678 Fragments arranged by size: 3678 3113 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 4751 5961 4127 Size: 624 1210 5025 Fragments arranged by size: 5025 1210 624 BclI T'GATC_A Cuts at: 1548 3040 1548 Size: 1492 5367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3152 5470 136 Size: 107 122 71 631 605 1480 2318 1525 Fragments arranged by size: 2318 1525 1480 631 605 122 107 71 BglII A'GATC_T Cuts at: 6854 6854 Size: 6859 BlpI GC'TnA_GC Cuts at: 4276 4276 Size: 6859 Bme1580I G_kGCm'C Cuts at: 1790 2174 2508 3168 3379 3517 4920 6076 1790 Size: 384 334 660 211 138 1403 1156 2573 Fragments arranged by size: 2573 1403 1156 660 384 334 211 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6859 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2950 3906 4590 Size: 416 53 83 399 24 1533 956 684 Cuts at: 4590 5548 442 Size: 958 1753 Fragments arranged by size: 1753 1533 958 956 684 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3193 3193 Size: 6859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3070 3698 5519 1239 Size: 1831 628 1821 2579 Fragments arranged by size: 2579 1831 1821 628 Bpu10I CC'TnA_GC Cuts at: 2099 2099 Size: 6859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3659 4984 5849 6147 3659 Size: 1325 865 298 4371 Fragments arranged by size: 4371 1325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5522 882 Size: 617 4023 2219 Fragments arranged by size: 4023 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4650 493 Size: 711 3446 2702 Fragments arranged by size: 3446 2702 711 BsaBI GATnn'nnATC Cuts at: 2613 6853 2613 Size: 4240 2619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2151 2180 2244 Size: 53 83 186 936 609 9 29 64 Cuts at: 2244 2271 4666 5048 5683 275 Size: 27 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 609 382 186 83 64 53 29 27 9 BsaWI w'CCGG_w Cuts at: 5292 5690 5964 6111 6522 5292 Size: 398 274 147 411 5629 Fragments arranged by size: 5629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 2772 2802 3801 Size: 30 165 30 840 30 499 30 999 Cuts at: 3801 3831 1178 Size: 30 4206 Fragments arranged by size: 4206 999 840 499 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2194 2206 1186 Size: 1008 12 5839 Fragments arranged by size: 5839 1008 12 BseYI C'CCAG_C Cuts at: 3137 3537 6062 6558 3137 Size: 400 2525 496 3438 Fragments arranged by size: 3438 2525 496 400 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3512 3699 1163 Size: 573 1776 187 4323 Fragments arranged by size: 4323 1776 573 187 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2261 4309 5070 6098 1540 Size: 275 186 260 2048 761 1028 2301 Fragments arranged by size: 2301 2048 1028 761 275 260 186 BsiHKAI G_wGCw'C Cuts at: 2750 3438 4150 4920 5005 6076 2750 Size: 688 712 770 85 1071 3533 Fragments arranged by size: 3533 1071 770 712 688 85 BsmI GAATG_Cn' Cuts at: 2554 2687 3412 3937 4370 4463 2554 Size: 133 725 525 433 93 4950 Fragments arranged by size: 4950 725 525 433 133 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2301 2508 2750 2834 3168 3379 Size: 399 384 127 207 242 84 334 211 Cuts at: 3379 3438 3517 4150 4178 4920 5005 6076 1391 Size: 59 79 633 28 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 633 399 384 334 242 211 207 127 85 84 79 59 28 BspEI T'CCGG_A Cuts at: 6522 6522 Size: 6859 BspHI T'CATG_A Cuts at: 2079 4748 6478 2079 Size: 2669 1730 2460 Fragments arranged by size: 2669 2460 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2536 2776 3256 4214 844 Size: 1692 240 480 958 3489 Fragments arranged by size: 3489 1692 958 480 240 BsrBI CCG'CTC Cuts at: 1394 1823 2092 4746 1394 Size: 429 269 2654 3507 Fragments arranged by size: 3507 2654 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 5354 5528 65 Size: 1183 4106 174 1396 Fragments arranged by size: 4106 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5503 5690 1700 Size: 110 3693 187 2869 Fragments arranged by size: 3693 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6859 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6859 BssSI C'ACGA_G Cuts at: 3167 4919 5931 3167 Size: 1752 1012 4095 Fragments arranged by size: 4095 1752 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3470 794 Size: 2676 4183 BstBI TT'CG_AA Cuts at: 5663 5663 Size: 6859 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6859 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6859 BstYI r'GATC_y Cuts at: 1972 3496 3634 4107 4189 4556 4941 4958 6410 Size: 1524 138 473 82 367 385 17 1452 Cuts at: 6410 6496 6508 6854 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1524 1452 473 385 367 346 138 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2035 2247 2730 2776 3156 513 Size: 552 970 212 483 46 380 4216 Fragments arranged by size: 4216 970 552 483 380 212 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2647 2768 3197 3471 3618 3954 4375 5169 5189 Size: 121 429 274 147 336 421 794 20 Cuts at: 5189 2647 Size: 4317 Fragments arranged by size: 4317 794 429 421 336 274 147 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6824 DraI TTT'AAA Cuts at: 4163 4163 Size: 6859 DraIII CAC_nnn'GTG Cuts at: 2441 2441 Size: 6859 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3841 4306 Size: 54 1425 50 275 18 580 1431 465 Cuts at: 4306 5195 6824 8 Size: 889 1629 43 Fragments arranged by size: 1629 1431 1425 889 580 465 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4306 1537 Size: 275 2494 4090 Fragments arranged by size: 4090 2494 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3190 4789 1353 Size: 1837 1599 3423 Fragments arranged by size: 3423 1837 1599 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2055 4001 5446 5820 5966 387 Size: 1064 138 466 1946 1445 374 146 1280 Fragments arranged by size: 1946 1445 1280 1064 466 374 146 138 EcoICRI GAG'CTC Cuts at: 2748 4148 2748 Size: 1400 5459 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2639 3070 3109 3575 3698 3762 3782 Size: 393 1007 431 39 466 123 64 20 Cuts at: 3782 3888 4142 4922 5519 6306 6574 1239 Size: 106 254 780 597 787 268 1524 Fragments arranged by size: 1524 1007 787 780 597 466 431 393 268 254 123 106 64 39 20 EcoO109I rG'GnC_Cy Cuts at: 2171 2330 2504 2171 Size: 159 174 6526 Fragments arranged by size: 6526 174 159 EcoRI G'AATT_C Cuts at: 4168 4546 4168 Size: 378 6481 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 2145 2183 2247 2274 2287 3147 6006 1678 Size: 467 38 64 27 13 860 2859 2531 Fragments arranged by size: 2859 2531 860 467 64 38 27 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2201 2233 2243 2275 2501 Size: 32 135 32 629 32 10 32 226 Cuts at: 2501 2533 2560 2592 2772 2804 4092 4124 5500 Size: 32 27 32 180 32 1288 32 1376 Cuts at: 5500 5532 5574 5606 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1376 1288 629 226 180 135 42 32 32 32 32 32 32 32 32 32 32 27 10 HincII GTy'rAC Cuts at: 669 1657 1938 3993 4203 4449 669 Size: 988 281 2055 210 246 3079 Fragments arranged by size: 3079 2055 988 281 246 210 HindIII A'AGCT_T Cuts at: 748 3019 748 Size: 2271 4588 HpaI GTT'AAC Cuts at: 4449 4449 Size: 6859 KasI G'GCGC_C Cuts at: 2141 2179 2243 2270 2141 Size: 38 64 27 6730 Fragments arranged by size: 6730 64 38 27 KpnI G_GTAC'C Cuts at: 4184 4184 Size: 6859 MfeI C'AATT_G Cuts at: 4458 4458 Size: 6859 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 2232 4135 5973 6157 1305 Size: 918 9 1903 1838 184 2007 Fragments arranged by size: 2007 1903 1838 918 184 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 10 Size: 54 1425 343 580 4457 Fragments arranged by size: 4457 1425 580 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2619 3561 4817 5176 5335 518 Size: 648 843 610 942 1256 359 159 2042 Fragments arranged by size: 2042 1256 942 843 648 610 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2157 3815 3866 4275 4952 6100 6345 Size: 431 120 1658 51 409 677 1148 245 Cuts at: 6345 1606 Size: 2120 Fragments arranged by size: 2120 1658 1148 677 431 409 245 120 51 NarI GG'CG_CC Cuts at: 2142 2180 2244 2271 2142 Size: 38 64 27 6730 Fragments arranged by size: 6730 64 38 27 NcoI C'CATG_G Cuts at: 513 1065 2776 3156 513 Size: 552 1711 380 4216 Fragments arranged by size: 4216 1711 552 380 NdeI CA'TA_TG Cuts at: 387 2304 387 Size: 1917 4942 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 2891 3421 3560 4217 5762 1132 Size: 97 1662 530 139 657 1545 2229 Fragments arranged by size: 2229 1662 1545 657 530 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6859 PciI A'CATG_T Cuts at: 5758 5758 Size: 6859 PflMI CCAn_nnn'nTGG Cuts at: 1108 2419 1108 Size: 1311 5548 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2964 2996 4928 4960 6441 6473 2964 Size: 32 1932 32 1481 32 3350 Fragments arranged by size: 3350 1932 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6859 PsiI TTA'TAA Cuts at: 4429 4429 Size: 6859 PspOMI G'GGCC_C Cuts at: 2170 2504 2170 Size: 334 6525 PstI C_TGCA'G Cuts at: 830 4211 830 Size: 3381 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 PvuII CAG'CTG Cuts at: 2157 3866 2157 Size: 1709 5150 SacI G_AGCT'C Cuts at: 2750 4150 2750 Size: 1400 5459 SacII CC_GC'GG Cuts at: 2038 2038 Size: 6859 SalI G'TCGA_C Cuts at: 1936 4201 1936 Size: 2265 4594 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 4045 5107 6819 4045 Size: 1062 1712 4085 Fragments arranged by size: 4085 1712 1062 SfcI C'TryA_G Cuts at: 826 1045 4207 5342 6023 6214 826 Size: 219 3162 1135 681 191 1471 Fragments arranged by size: 3162 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2143 2181 2245 2272 2143 Size: 38 64 27 6730 Fragments arranged by size: 6730 64 38 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6859 SmaI CCC'GGG Cuts at: 1872 2139 2593 4186 1872 Size: 267 454 1593 4545 Fragments arranged by size: 4545 1593 454 267 SmlI C'TyrA_G Cuts at: 820 1017 1943 3638 4963 5864 6126 820 Size: 197 926 1695 1325 901 262 1553 Fragments arranged by size: 1695 1553 1325 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4650 493 Size: 4157 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6859 SphI G_CATG'C Cuts at: 1132 4217 1132 Size: 3085 3774 SspI AAT'ATT Cuts at: 5 52 3061 3298 4783 5 Size: 47 3009 237 1485 2081 Fragments arranged by size: 3009 2081 1485 237 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6859 StyI C'CwwG_G Cuts at: 513 1065 2776 3156 3364 4298 513 Size: 552 1711 380 208 934 3074 Fragments arranged by size: 3074 1711 934 552 380 208 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2152 2927 4886 4903 5056 5241 5686 Size: 1255 572 775 1959 17 153 185 445 Cuts at: 5686 325 Size: 1498 Fragments arranged by size: 1959 1498 1255 775 572 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5105 6817 Size: 274 80 33 51 1443 2066 1062 1712 Cuts at: 6817 96 Size: 138 Fragments arranged by size: 2066 1712 1443 1062 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3801 3833 6819 6851 3801 Size: 32 2986 32 3809 Fragments arranged by size: 3809 2986 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6859 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2309 2309 Size: 6859 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6859 XmaI C'CCGG_G Cuts at: 1870 2137 2591 4184 1870 Size: 267 454 1593 4545 Fragments arranged by size: 4545 1593 454 267 XmnI GAAnn'nnTTC Cuts at: 2610 3888 4988 2610 Size: 1278 1100 4481 Fragments arranged by size: 4481 1278 1100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4667 276 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI