################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AgeI AhdI ApaI AscI Asp718I AspEI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MluI MluNI MroI MscI NaeI NarI NciI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI StyI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AclI 1315 AACGTT AA^CGTT AflIII 310 ACATGT A^C[GA][CT]GT AlwNI 1966 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1639 AGCGCT AGC^GCT Asp700I 581 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1090 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 104 GGATCC G^GATCC BciVI 321 GGATAC GTATCC(6/5) BclI 98 TGATCA T^GATCA BpmI 87 CTGGAG CTGGAG(16/14) BsaWI 353 TCCGGT [AT]^CCGG[AT] BseRI 1447 CTCCTC GAGGAG(10/8) BsiEI 2254 CGGCCG CG[GA][CT]^CG BsiYI 328 CCAAATGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 328 CCAAATGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspLU11I 310 ACATGT A^CATGT BspMI 444 GCAGGT ACCTGC(4/8) BsrFI 1808 ACCGGC [GA]^CCGG[CT] BstYI 104 GGATCC [GA]^GATC[CT] Cfr10I 1808 ACCGGC [GA]^CCGG[CT] DraII 804 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 2254 CGGCCG [CT]^GGCC[GA] EagI 2254 CGGCCG C^GGCCG EclXI 2254 CGGCCG C^GGCCG Eco47III 1639 AGCGCT AGC^GCT Eco52I 2254 CGGCCG C^GGCCG EcoO109I 804 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 334 GCGGG CCCGC(4/6) FbaI 98 TGATCA T^GATCA HaeII 1639 AGCGCT [GA]GCGC^[CT] HgaI 2250 GACGC GACGC(5/10) MaeII 1316 ACGT A^CGT MfeI 224 CAATTG C^AATTG MflI 104 GGATCC [GA]^GATC[CT] MunI 224 CAATTG C^AATTG NdeI 1184 CATATG CA^TATG NheI 841 GCTAGC G^CTAGC PflFI 1090 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 1315 AACGTT AA^CGTT PstI 153 CTGCAG CTGCA^G ScaI 2341 AGTACT AGT^ACT SmlI 1044 CTCAAG C^T[CT][GA]AG SpeI 538 ACTAGT A^CTAGT SspI 1513 AATATT AAT^ATT TaqI 1939 TCGA T^CGA Tth111I 1090 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1939 TCGA T^CGA XcmI 272 CCAGAATCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 104 GGATCC [GA]^GATC[CT] XmnI 581 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 548 CGCG CG^CG 2252 CGCG Alw44I 1325 GTGCAC G^TGCAC 1575 GTGCAC ApaLI 1325 GTGCAC G^TGCAC 1575 GTGCAC AseI 1260 ATTAAT AT^TAAT 1713 ATTAAT AsnI 1260 ATTAAT AT^TAAT 1713 ATTAAT AspHI 1325 GTGCAC G[AT]GC[AT]^C 1575 GTGCAC AvaII 295 GGACC G^G[AT]CC 1670 GGACC BbsI 2098 GTCTTC GAAGAC(2/6) 2176 GTCTTC BcgI 1940 CGATCAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2087 GCATCAGGATCG BpuAI 2098 GTCTTC GAAGAC(2/6) 2176 GTCTTC BsaBI 93 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 99 GATCAGGATC BsiHKAI 1325 GTGCAC G[AT]GC[AT]^C 1575 GTGCAC BsmAI 715 GAGAC GTCTC(1/5) 365 GTCTC BstUI 548 CGCG CG^CG 2252 CGCG CfoI 1224 GCGC GCG^C 1640 GCGC HapII 354 CCGG C^CGG 1809 CCGG HhaI 1224 GCGC GCG^C 1640 GCGC HinP1I 1224 GCGC G^CGC 1640 GCGC HincII 626 GTCAAC GT[CT]^[GA]AC 1087 GTTGAC HindII 626 GTCAAC GT[CT]^[GA]AC 1087 GTTGAC HindIII 988 AAGCTT A^AGCTT 2189 AAGCTT HpaII 354 CCGG C^CGG 1809 CCGG MamI 93 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 99 GATCAGGATC MslI 877 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1425 CATATCAATG MspA1I 1230 CCGCTG C[AC]G^C[GT]G 1867 CCGCTG MspI 354 CCGG C^CGG 1809 CCGG MvnI 548 CGCG CG^CG 2252 CGCG NspI 310 ACATGT [GA]CATG^[CT] 958 ACATGC PleI 624 GAGTC GAGTC(4/5) 1727 GAGTC PshBI 1260 ATTAAT AT^TAAT 1713 ATTAAT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI BbvI BfaI BmyI BsaJI BsgI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII EarI Eco81I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TfiI Tru9I TseI Tsp45I Tsp509I TspRI