(Circular) (Six-base) MAPSORT of: pFN21AE9426.seq Check: 8889 from: 1 to: 7096 pFN21AE9426 7096 bp With 182 enzymes: SgfI * March 28, 2012 11:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4906 278 Size: 53 83 186 936 3370 2468 Fragments arranged by size: 3370 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4439 1937 Size: 2502 4594 Acc65I G'GTAC_C Cuts at: 4417 4417 Size: 7096 AclI AA'CG_TT Cuts at: 1563 3321 5223 5596 1563 Size: 1758 1902 373 3063 Fragments arranged by size: 3063 1902 1758 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2511 2778 2909 4090 4233 5159 6543 6811 Size: 879 267 131 1181 143 926 1384 268 Cuts at: 6811 1632 Size: 1917 Fragments arranged by size: 1917 1384 1181 926 879 268 267 143 131 AfeI AGC'GCT Cuts at: 3646 3646 Size: 7096 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6899 AflIII A'CryG_T Cuts at: 1203 2315 5995 6972 1203 Size: 1112 3680 977 1327 Fragments arranged by size: 3680 1327 1112 977 AgeI A'CCGG_T Cuts at: 5927 5927 Size: 7096 AhdI GACnn_n'nnGTC Cuts at: 5825 5825 Size: 7096 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7096 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7096 AlwNI CAG_nnn'CTG Cuts at: 3976 4839 6411 3976 Size: 863 1572 4661 Fragments arranged by size: 4661 1572 863 ApaLI G'TGCA_C Cuts at: 3330 3580 5153 6309 3330 Size: 250 1573 1156 4117 Fragments arranged by size: 4117 1573 1156 250 ApoI r'AATT_y Cuts at: 1493 2785 3128 3163 4149 4405 4631 4783 6892 Size: 1292 343 35 986 256 226 152 2109 Cuts at: 6892 1493 Size: 1697 Fragments arranged by size: 2109 1697 1292 986 343 256 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 3266 3719 5650 160 Size: 872 2234 453 1931 1606 Fragments arranged by size: 2234 1931 1606 872 453 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7096 AvaI C'yCGr_G Cuts at: 1870 1943 4421 1870 Size: 73 2478 4545 Fragments arranged by size: 4545 2478 73 BamHI G'GATC_C Cuts at: 2109 4426 4793 2109 Size: 2317 367 4412 Fragments arranged by size: 4412 2317 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4417 5872 618 Size: 325 217 627 2630 1455 1842 Fragments arranged by size: 2630 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4415 1391 Size: 3024 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4096 4174 928 Size: 971 2197 78 3850 Fragments arranged by size: 3850 2197 971 78 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 4081 4115 5287 5321 1903 Size: 34 1997 34 113 34 1172 34 3678 Fragments arranged by size: 3678 1997 1172 113 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2320 4988 6198 2320 Size: 2668 1210 3218 Fragments arranged by size: 3218 2668 1210 BclI T'GATC_A Cuts at: 1548 2103 1548 Size: 555 6541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5707 136 Size: 107 122 71 631 605 4035 1525 Fragments arranged by size: 4035 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7091 7091 Size: 7096 BlpI GC'TnA_GC Cuts at: 4513 4513 Size: 7096 Bme1580I G_kGCm'C Cuts at: 1790 3215 3334 3584 5157 6313 1790 Size: 1425 119 250 1573 1156 2573 Fragments arranged by size: 2573 1573 1425 1156 250 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7096 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2093 2279 3190 Size: 416 53 83 399 24 676 186 911 Cuts at: 3190 4827 5785 442 Size: 1637 958 1753 Fragments arranged by size: 1753 1637 958 911 676 416 399 186 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2850 1056 Size: 1794 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2113 5756 1239 Size: 874 3643 2579 Fragments arranged by size: 3643 2579 874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3034 5221 6086 6384 3034 Size: 2187 865 298 3746 Fragments arranged by size: 3746 2187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5759 882 Size: 617 4260 2219 Fragments arranged by size: 4260 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4887 493 Size: 711 3683 2702 Fragments arranged by size: 3683 2702 711 BsaBI GATnn'nnATC Cuts at: 2102 2108 7090 2102 Size: 6 4982 2108 Fragments arranged by size: 4982 2108 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4903 5285 5920 275 Size: 53 83 186 936 3370 382 635 1451 Fragments arranged by size: 3370 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 5529 5927 6201 6348 6759 2358 Size: 3171 398 274 147 411 2695 Fragments arranged by size: 3171 2695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6871 Fragments arranged by size: 6871 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3443 1186 Size: 2257 4839 BseYI C'CCAG_C Cuts at: 3736 6299 6795 3736 Size: 2563 496 4037 Fragments arranged by size: 4037 2563 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2386 2855 3587 3633 1163 Size: 573 650 469 732 46 4626 Fragments arranged by size: 4626 732 650 573 469 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4262 4546 5307 6335 1540 Size: 275 186 2261 284 761 1028 2301 Fragments arranged by size: 2301 2261 1028 761 284 275 186 BsiHKAI G_wGCw'C Cuts at: 3334 3584 5157 5242 6313 3334 Size: 250 1573 85 1071 4117 Fragments arranged by size: 4117 1573 1071 250 85 BsmI GAATG_Cn' Cuts at: 4607 4700 4607 Size: 93 7003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3215 3334 3584 4415 5157 5242 6313 Size: 399 1425 119 250 831 742 85 1071 Cuts at: 6313 1391 Size: 2174 Fragments arranged by size: 2174 1425 1071 831 742 399 250 119 85 BspEI T'CCGG_A Cuts at: 6759 6759 Size: 7096 BspHI T'CATG_A Cuts at: 4985 6715 4985 Size: 1730 5366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 4451 844 Size: 1596 2011 3489 Fragments arranged by size: 3489 2011 1596 BsrBI CCG'CTC Cuts at: 1394 1823 4983 1394 Size: 429 3160 3507 Fragments arranged by size: 3507 3160 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2428 3925 4241 4298 5591 5765 65 Size: 1183 1180 1497 316 57 1293 174 1396 Fragments arranged by size: 1497 1396 1293 1183 1180 316 174 57 BsrFI r'CCGG_y Cuts at: 1700 1810 3813 5740 5927 1700 Size: 110 2003 1927 187 2869 Fragments arranged by size: 2869 2003 1927 187 110 BsrGI T'GTAC_A Cuts at: 96 2198 2225 4161 96 Size: 2102 27 1936 3031 Fragments arranged by size: 3031 2102 1936 27 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7096 BssSI C'ACGA_G Cuts at: 5156 6168 5156 Size: 1012 6084 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7096 BstBI TT'CG_AA Cuts at: 5900 5900 Size: 7096 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7096 BstYI r'GATC_y Cuts at: 1972 2109 4426 4793 5178 5195 6647 6733 6745 Size: 137 2317 367 385 17 1452 86 12 Cuts at: 6745 7091 1972 Size: 346 1977 Fragments arranged by size: 2317 1977 1452 385 367 346 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2030 2598 4292 2030 Size: 568 1694 4834 Fragments arranged by size: 4834 1694 568 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5605 Fragments arranged by size: 5605 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6357 Fragments arranged by size: 6357 683 56 BtsI GCAGTG_nn' Cuts at: 3582 4612 5406 5426 3582 Size: 1030 794 20 5252 Fragments arranged by size: 5252 1030 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7096 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7061 DraI TTT'AAA Cuts at: 4400 4400 Size: 7096 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6103 809 Size: 507 4787 1802 Fragments arranged by size: 4787 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4259 4543 5432 Size: 54 1425 50 275 18 2429 284 889 Cuts at: 5432 7061 8 Size: 1629 43 Fragments arranged by size: 2429 1629 1425 889 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4259 4543 1537 Size: 275 2447 284 4090 Fragments arranged by size: 4090 2447 284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2797 3693 5026 1353 Size: 846 598 896 1333 3423 Fragments arranged by size: 3423 1333 896 846 598 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5683 6057 6203 387 Size: 1064 138 4094 374 146 1280 Fragments arranged by size: 4094 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2113 2511 2778 2909 4090 4233 5159 Size: 393 481 398 267 131 1181 143 926 Cuts at: 5159 5756 6543 6811 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1181 926 787 597 481 398 393 268 267 143 131 EcoO109I rG'GnC_Cy Cuts at: 2810 2810 Size: 7096 EcoRI G'AATT_C Cuts at: 4405 4783 4405 Size: 378 6718 FspI TGC'GCA Cuts at: 5602 5602 Size: 7096 HaeII r_GCGC'y Cuts at: 1678 3648 6243 1678 Size: 1970 2595 2531 Fragments arranged by size: 2595 2531 1970 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 3299 3331 3717 Size: 32 135 32 501 32 1194 32 386 Cuts at: 3717 3749 5737 5769 5811 5843 1373 Size: 32 1988 32 42 32 2626 Fragments arranged by size: 2626 1988 1194 501 386 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2633 3094 4440 4686 669 Size: 988 281 695 461 1346 246 3079 Fragments arranged by size: 3079 1346 988 695 461 281 246 HindIII A'AGCT_T Cuts at: 748 2993 4194 748 Size: 2245 1201 3650 Fragments arranged by size: 3650 2245 1201 HpaI GTT'AAC Cuts at: 4686 4686 Size: 7096 KpnI G_GTAC'C Cuts at: 4421 4421 Size: 7096 MfeI C'AATT_G Cuts at: 2229 4695 2229 Size: 2466 4630 MluI A'CGCG_T Cuts at: 6972 6972 Size: 7096 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2542 3304 3342 6210 6394 1305 Size: 1237 762 38 2868 184 2007 Fragments arranged by size: 2868 2007 1237 762 184 38 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5274 Fragments arranged by size: 5274 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2886 3434 5054 5413 5572 518 Size: 648 1720 548 1620 359 159 2042 Fragments arranged by size: 2042 1720 1620 648 548 359 159 MspA1I CmG'CkG Cuts at: 1606 3237 3874 4512 5189 6337 6582 1606 Size: 1631 637 638 677 1148 245 2120 Fragments arranged by size: 2120 1631 1148 677 638 637 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5605 Fragments arranged by size: 5605 939 552 NdeI CA'TA_TG Cuts at: 387 3190 387 Size: 2803 4293 NheI G'CTAG_C Cuts at: 1052 2846 1052 Size: 1794 5302 NotI GC'GGCC_GC Cuts at: 4543 4543 Size: 7096 NspI r_CATG'y Cuts at: 1132 1229 2319 2967 4454 5999 1132 Size: 97 1090 648 1487 1545 2229 Fragments arranged by size: 2229 1545 1487 1090 648 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7096 PciI A'CATG_T Cuts at: 2315 5995 2315 Size: 3680 3416 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7096 PmeI GTTT'AAAC Cuts at: 4400 4400 Size: 7096 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5165 5197 6678 6710 5165 Size: 32 1481 32 5551 Fragments arranged by size: 5551 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7096 PsiI TTA'TAA Cuts at: 3863 4666 3863 Size: 803 6293 PstI C_TGCA'G Cuts at: 830 2162 4448 830 Size: 1332 2286 3478 Fragments arranged by size: 3478 2286 1332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7096 SalI G'TCGA_C Cuts at: 1936 4438 1936 Size: 2502 4594 SbfI CC_TGCA'GG Cuts at: 4448 4448 Size: 7096 ScaI AGT'ACT Cuts at: 4348 5344 7056 4348 Size: 996 1712 4388 Fragments arranged by size: 4388 1712 996 SfcI C'TryA_G Cuts at: 826 1045 2148 2158 3741 4444 5579 6260 6451 Size: 219 1103 10 1583 703 1135 681 191 Cuts at: 6451 826 Size: 1471 Fragments arranged by size: 1583 1471 1135 1103 703 681 219 191 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7096 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7096 SmaI CCC'GGG Cuts at: 1872 4423 1872 Size: 2551 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3049 5200 6101 6363 820 Size: 197 926 1106 2151 901 262 1553 Fragments arranged by size: 2151 1553 1106 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4887 493 Size: 4394 2702 SpeI A'CTAG_T Cuts at: 152 2543 152 Size: 2391 4705 SphI G_CATG'C Cuts at: 1132 4454 1132 Size: 3322 3774 SspI AAT'ATT Cuts at: 5 52 3520 5020 5 Size: 47 3468 1500 2081 Fragments arranged by size: 3468 2081 1500 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7096 StyI C'CwwG_G Cuts at: 513 1065 2004 4535 513 Size: 552 939 2531 3074 Fragments arranged by size: 3074 2531 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5123 5140 5293 5478 5923 325 Size: 1255 3543 17 153 185 445 1498 Fragments arranged by size: 3543 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2198 2225 3114 Size: 274 80 33 51 1443 221 27 889 Cuts at: 3114 4161 4346 5342 7054 96 Size: 1047 185 996 1712 138 Fragments arranged by size: 1712 1443 1047 996 889 274 221 185 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7056 7088 7056 Size: 32 7064 Tth111I GACn'n_nGTC Cuts at: 1316 3098 1316 Size: 1782 5314 XbaI T'CTAG_A Cuts at: 4432 4432 Size: 7096 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 7096 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7096 XmaI C'CCGG_G Cuts at: 1870 4421 1870 Size: 2551 4545 XmnI GAAnn'nnTTC Cuts at: 2590 5225 2590 Size: 2635 4461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4904 276 Size: 53 83 186 936 3370 2468 Fragments arranged by size: 3370 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI