################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 328 GTAGAC GT^[AC][GT]AC AfaI 531 GTAC GT^AC AflIII 437 ACACGT A^C[GA][CT]GT AluI 258 AGCT AG^CT AlwI 413 GGATC GGATC(4/5) AlwNI 142 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 214 ATTAAT AT^TAAT AsnI 214 ATTAAT AT^TAAT Asp700I 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 257 GAGCTC G[AT]GC[AT]^C AvaII 63 GGACC G^G[AT]CC AvrII 384 CCTAGG C^CTAGG BanII 257 GAGCTC G[GA]GC[CT]^C BbsI 165 GAAGAC GAAGAC(2/6) BfaI 385 CTAG C^TAG BlnI 384 CCTAGG C^CTAGG BmyI 257 GAGCTC G[AGT]GC[ACT]^C BpuAI 165 GAAGAC GAAGAC(2/6) BsiHKAI 257 GAGCTC G[AT]GC[AT]^C Bsp1286I 257 GAGCTC G[AGT]GC[ACT]^C BspMI 65 ACCTGC ACCTGC(4/8) BsrBI 79 CCGCTC CCGCTC(-3/-3) BstXI 272 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 63 GGACC G^G[ACGT]CC DpnII 414 GATC ^GATC DraI 201 TTTAAA TTT^AAA DraII 62 GGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 247 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 62 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 91 GAATTC G^AATTC EcoT14I 384 CCTAGG C^C[AT][AT]GG MaeI 385 CTAG C^TAG MaeII 439 ACGT A^CGT MboI 414 GATC ^GATC MwoI 69 GCCCAGGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 414 GATC ^GATC PflMI 36 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 62 GGGACCT [GA]G^G[AT]CC[CT] PshBI 214 ATTAAT AT^TAAT RsaI 531 GTAC GT^AC SacI 257 GAGCTC GAGCT^C Sau3AI 414 GATC ^GATC Sau96I 63 GGACC G^G[ACGT]CC SexAI 169 ACCTGGT A^CC[AT]GGT StyI 384 CCTAGG C^C[AT][AT]GG TaqI 195 TCGA T^CGA TthHB8I 195 TCGA T^CGA Van91I 36 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 76 CCGC CCGC(-3/-1) 79 CCGC AcsI 91 GAATTC [GA]^AATT[CT] 535 AAATTT ApoI 91 GAATTC [GA]^AATT[CT] 535 AAATTT BsiYI 36 CCAAAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 247 CCTCCACAAGG BslI 36 CCAAAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 247 CCTCCACAAGG BsmFI 62 GGGAC GGGAC(10/14) 33 GTCCC BstF5I 282 GGATG GGATG(2/0) 555 GGATG Fnu4HI 75 GCCGC GC^[ACGT]GC 78 GCCGC FokI 282 GGATG GGATG(9/13) 555 GGATG HaeIII 74 GGCC GG^CC 388 GGCC HphI 150 GGTGA GGTGA(8/7) 381 TCACC ItaI 75 GCCGC GC^[ACGT]GC 78 GCCGC MaeIII 406 GTGAC ^GT[ACGT]AC 434 GTGAC NlaIV 62 GGGACC GG[ACGT]^[ACGT]CC 243 GGAACC Tsp45I 406 GTGAC ^GT[GC]AC 434 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BsaJI BssKI BstNI DdeI EcoRII HinfI MboII MnlI MseI MvaI NlaIII ScrFI SfaNI TfiI Tru9I Tsp509I