(Circular) (Six-base) MAPSORT of: pFN21AE5245.seq Check: 5344 from: 1 to: 5266 pFN21AE5245 5266 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3076 278 Size: 53 83 186 936 1540 2468 Fragments arranged by size: 2468 1540 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 2609 1937 Size: 397 275 4594 Fragments arranged by size: 4594 397 275 Acc65I G'GTAC_C Cuts at: 2587 2587 Size: 5266 AclI AA'CG_TT Cuts at: 1563 3393 3766 1563 Size: 1830 373 3063 Fragments arranged by size: 3063 1830 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 3329 4713 4981 1632 Size: 483 1214 1384 268 1917 Fragments arranged by size: 1917 1384 1214 483 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5069 AflIII A'CryG_T Cuts at: 1203 2442 4165 5142 1203 Size: 1239 1723 977 1327 Fragments arranged by size: 1723 1327 1239 977 AgeI A'CCGG_T Cuts at: 4097 4097 Size: 5266 AhdI GACnn_n'nnGTC Cuts at: 3995 3995 Size: 5266 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5266 AlwNI CAG_nnn'CTG Cuts at: 2152 3009 4581 2152 Size: 857 1572 2837 Fragments arranged by size: 2837 1572 857 ApaLI G'TGCA_C Cuts at: 3323 4479 3323 Size: 1156 4110 ApoI r'AATT_y Cuts at: 1493 2096 2540 2575 2801 2953 5062 1493 Size: 603 444 35 226 152 2109 1697 Fragments arranged by size: 2109 1697 603 444 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 2220 3820 160 Size: 872 1188 1600 1606 Fragments arranged by size: 1606 1600 1188 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5266 AvaI C'yCGr_G Cuts at: 1870 1943 2591 1870 Size: 73 648 4545 Fragments arranged by size: 4545 648 73 AvrII C'CTAG_G Cuts at: 2389 2389 Size: 5266 BamHI G'GATC_C Cuts at: 2596 2963 2596 Size: 367 4899 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2587 4042 618 Size: 325 217 627 800 1455 1842 Fragments arranged by size: 1842 1455 800 627 325 217 BanII G_rGCy'C Cuts at: 1391 2266 2585 1391 Size: 875 319 4072 Fragments arranged by size: 4072 875 319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2177 928 Size: 971 278 4017 Fragments arranged by size: 4017 971 278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3457 3491 1903 Size: 34 1520 34 3678 Fragments arranged by size: 3678 1520 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3158 4368 3158 Size: 1210 4056 BclI T'GATC_A Cuts at: 1548 1548 Size: 5266 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3877 136 Size: 107 122 71 631 605 2205 1525 Fragments arranged by size: 2205 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5261 5261 Size: 5266 BlpI GC'TnA_GC Cuts at: 2683 2683 Size: 5266 Bme1580I G_kGCm'C Cuts at: 1790 3327 4483 1790 Size: 1537 1156 2573 Fragments arranged by size: 2573 1537 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5266 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2997 3955 442 Size: 416 53 83 399 24 1580 958 1753 Fragments arranged by size: 1753 1580 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3926 1239 Size: 2687 2579 Bpu10I CC'TnA_GC Cuts at: 2087 2087 Size: 5266 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3391 4256 4554 3391 Size: 865 298 4103 Fragments arranged by size: 4103 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3929 882 Size: 617 2430 2219 Fragments arranged by size: 2430 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3057 493 Size: 711 1853 2702 Fragments arranged by size: 2702 1853 711 BsaBI GATnn'nnATC Cuts at: 5260 5260 Size: 5266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3073 3455 4090 275 Size: 53 83 186 936 1540 382 635 1451 Fragments arranged by size: 1540 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3699 4097 4371 4518 4929 3699 Size: 398 274 147 411 4036 Fragments arranged by size: 4036 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5041 Fragments arranged by size: 5041 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5266 BseYI C'CCAG_C Cuts at: 4469 4965 4469 Size: 496 4770 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4693 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2716 3477 4505 1540 Size: 275 186 715 761 1028 2301 Fragments arranged by size: 2301 1028 761 715 275 186 BsiHKAI G_wGCw'C Cuts at: 2266 3327 3412 4483 2266 Size: 1061 85 1071 3049 Fragments arranged by size: 3049 1071 1061 85 BsmI GAATG_Cn' Cuts at: 2777 2870 2777 Size: 93 5173 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2585 3327 3412 4483 1391 Size: 399 476 319 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 399 319 85 BspEI T'CCGG_A Cuts at: 4929 4929 Size: 5266 BspHI T'CATG_A Cuts at: 3155 4885 3155 Size: 1730 3536 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2621 844 Size: 1235 542 3489 Fragments arranged by size: 3489 1235 542 BsrBI CCG'CTC Cuts at: 1394 1823 2086 3153 1394 Size: 429 263 1067 3507 Fragments arranged by size: 3507 1067 429 263 BsrDI GCAATG_nn' Cuts at: 65 1248 3761 3935 65 Size: 1183 2513 174 1396 Fragments arranged by size: 2513 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3910 4097 1700 Size: 110 2100 187 2869 Fragments arranged by size: 2869 2100 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5266 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5266 BssSI C'ACGA_G Cuts at: 3326 4338 3326 Size: 1012 4254 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5266 BstBI TT'CG_AA Cuts at: 4070 4070 Size: 5266 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5266 BstXI CCAn_nnnn'nTGG Cuts at: 2284 2284 Size: 5266 BstYI r'GATC_y Cuts at: 1972 2596 2963 3348 3365 4817 4903 4915 5261 Size: 624 367 385 17 1452 86 12 346 Cuts at: 5261 1972 Size: 1977 Fragments arranged by size: 1977 1452 624 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4714 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4527 Fragments arranged by size: 4527 683 56 BtsI GCAGTG_nn' Cuts at: 2782 3576 3596 2782 Size: 794 20 4452 Fragments arranged by size: 4452 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5231 DraI TTT'AAA Cuts at: 2208 2570 2208 Size: 362 4904 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4273 809 Size: 507 2957 1802 Fragments arranged by size: 2957 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2713 3602 5231 Size: 54 1425 50 275 18 883 889 1629 Cuts at: 5231 8 Size: 43 Fragments arranged by size: 1629 1425 889 883 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2713 1537 Size: 275 901 4090 Fragments arranged by size: 4090 901 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3196 1353 Size: 1843 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3853 4227 4373 387 Size: 1064 138 2264 374 146 1280 Fragments arranged by size: 2264 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2264 2264 Size: 5266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 3329 3926 4713 4981 1239 Size: 393 483 1214 597 787 268 1524 Fragments arranged by size: 1524 1214 787 597 483 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2256 2256 Size: 5266 EcoO109I rG'GnC_Cy Cuts at: 2068 2068 Size: 5266 EcoRI G'AATT_C Cuts at: 2096 2575 2953 2096 Size: 479 378 4409 Fragments arranged by size: 4409 479 378 FspI TGC'GCA Cuts at: 3772 3772 Size: 5266 HaeII r_GCGC'y Cuts at: 1678 4413 1678 Size: 2735 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3907 3939 3981 4013 1373 Size: 32 135 32 2335 32 42 32 2626 Fragments arranged by size: 2626 2335 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2610 2856 669 Size: 988 281 672 246 3079 Fragments arranged by size: 3079 988 672 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5266 HpaI GTT'AAC Cuts at: 2856 2856 Size: 5266 KpnI G_GTAC'C Cuts at: 2591 2591 Size: 5266 MfeI C'AATT_G Cuts at: 2865 2865 Size: 5266 MluI A'CGCG_T Cuts at: 5142 5142 Size: 5266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4380 4564 1305 Size: 3075 184 2007 Fragments arranged by size: 3075 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3444 Fragments arranged by size: 3444 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3224 3583 3742 518 Size: 648 2058 359 159 2042 Fragments arranged by size: 2058 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2682 3359 4507 4752 1606 Size: 1076 677 1148 245 2120 Fragments arranged by size: 2120 1148 1076 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4714 NdeI CA'TA_TG Cuts at: 387 387 Size: 5266 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5266 NotI GC'GGCC_GC Cuts at: 2713 2713 Size: 5266 NspI r_CATG'y Cuts at: 1132 1229 2624 4169 1132 Size: 97 1395 1545 2229 Fragments arranged by size: 2229 1545 1395 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5266 PciI A'CATG_T Cuts at: 4165 4165 Size: 5266 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 1108 Size: 939 4327 PfoI T'CCnGG_A Cuts at: 2514 2514 Size: 5266 PmeI GTTT'AAAC Cuts at: 2570 2570 Size: 5266 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3335 3367 4848 4880 3335 Size: 32 1481 32 3721 Fragments arranged by size: 3721 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2068 Size: 5266 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5266 PsiI TTA'TAA Cuts at: 2370 2836 2370 Size: 466 4800 PstI C_TGCA'G Cuts at: 830 2618 830 Size: 1788 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5266 SacI G_AGCT'C Cuts at: 2266 2266 Size: 5266 SalI G'TCGA_C Cuts at: 1936 2608 1936 Size: 672 4594 SbfI CC_TGCA'GG Cuts at: 2618 2618 Size: 5266 ScaI AGT'ACT Cuts at: 3514 5226 3514 Size: 1712 3554 SexAI A'CCwGG_T Cuts at: 2174 2174 Size: 5266 SfcI C'TryA_G Cuts at: 826 1045 2614 3749 4430 4621 826 Size: 219 1569 1135 681 191 1471 Fragments arranged by size: 1569 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5266 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5266 SmaI CCC'GGG Cuts at: 1872 2593 1872 Size: 721 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3370 4271 4533 820 Size: 197 926 1427 901 262 1553 Fragments arranged by size: 1553 1427 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3057 493 Size: 2564 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5266 SphI G_CATG'C Cuts at: 1132 2624 1132 Size: 1492 3774 SspI AAT'ATT Cuts at: 5 52 3190 5 Size: 47 3138 2081 Fragments arranged by size: 3138 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5266 StyI C'CwwG_G Cuts at: 513 1065 2389 2705 513 Size: 552 1324 316 3074 Fragments arranged by size: 3074 1324 552 316 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3293 3310 3463 3648 4093 325 Size: 1255 1713 17 153 185 445 1498 Fragments arranged by size: 1713 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2535 3512 5224 Size: 274 80 33 51 1443 558 977 1712 Cuts at: 5224 96 Size: 138 Fragments arranged by size: 1712 1443 977 558 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2338 2370 5226 5258 2338 Size: 32 2856 32 2346 Fragments arranged by size: 2856 2346 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5266 XbaI T'CTAG_A Cuts at: 2602 2602 Size: 5266 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5266 XmaI C'CCGG_G Cuts at: 1870 2591 1870 Size: 721 4545 XmnI GAAnn'nnTTC Cuts at: 2096 3395 2096 Size: 1299 3967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3074 276 Size: 53 83 186 936 1540 2468 Fragments arranged by size: 2468 1540 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI