(Linear) (Six-base) MAPSORT of: KIEE5245.seq  Check: 6969  from: 1  to: 561



KIEE5245.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AccI GT'mk_AC

Cuts at:      0     329     561 
   Size:        329     232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     561 
   Size:        110     451
 
AflIII A'CryG_T

Cuts at:      0     437     561 
   Size:        437     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     147     561 
   Size:        147     414
 
ApoI r'AATT_y

Cuts at:      0      91     535     561 
   Size:         91     444      26

  Fragments arranged by size: 

                 444      91      26
 
AseI AT'TA_AT

Cuts at:      0     215     561 
   Size:        215     346
 
AvrII C'CTAG_G

Cuts at:      0     384     561 
   Size:        384     177
 
BanII G_rGCy'C

Cuts at:      0     261     561 
   Size:        261     300
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     172     561 
   Size:        172     389
 
Bpu10I CC'TnA_GC

Cuts at:      0      82     561 
   Size:         82     479
 
BsiHKAI G_wGCw'C

Cuts at:      0     261     561 
   Size:        261     300
 
Bsp1286I G_dGCh'C

Cuts at:      0     261     561 
   Size:        261     300
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     561 
   Size:         74     487
 
BsrBI CCG'CTC

Cuts at:      0      81     561 
   Size:         81     480
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     279     561 
   Size:        279     282
 
DraI TTT'AAA

Cuts at:      0     203     561 
   Size:        203     358
 
EcoICRI GAG'CTC

Cuts at:      0     259     561 
   Size:        259     302
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     561 
   Size:        110     451
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     251     561 
   Size:        251     310
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     561 
   Size:         63     498
 
EcoRI G'AATT_C

Cuts at:      0      91     561 
   Size:         91     470
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42     561 
   Size:         42     519
 
PfoI T'CCnGG_A

Cuts at:      0     509     561 
   Size:        509      52
 
PpuMI rG'GwC_Cy

Cuts at:      0      63     561 
   Size:         63     498
 
PsiI TTA'TAA

Cuts at:      0     365     561 
   Size:        365     196
 
SacI G_AGCT'C

Cuts at:      0     261     561 
   Size:        261     300
 
SexAI A'CCwGG_T

Cuts at:      0     169     561 
   Size:        169     392
 
StyI C'CwwG_G

Cuts at:      0     384     561 
   Size:        384     177
 
TatI w'GTAC_w

Cuts at:      0     530     561 
   Size:        530      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     333     365     561 
   Size:        333      32     196

  Fragments arranged by size: 

                 333     196      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      91     561 
   Size:         91     470
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AseI    AvrII    BanII
     BbsI   Bpu10I  BsiHKAI Bsp1286I    BspMI    BsrBI    BstXI     DraI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    PflMI     PfoI    PpuMI
     PsiI     SacI    SexAI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI