################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 561 ACATGT A^C[GA][CT]GT AlwNI 18 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 762 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 504 CTCGGG C^[CT]CG[GA]G AvaII 747 GGACC G^G[AT]CC AvrII 352 CCTAGG C^CTAGG BcnI 1071 CCGGG CC^[GC]GG BlnI 352 CCTAGG C^CTAGG BseRI 1133 CTCCTC GAGGAG(10/8) BsmFI 81 GGGAC GGGAC(10/14) BsoBI 504 CTCGGG C^[CT]CG[GA]G BspHI 1269 TCATGA T^CATGA BspLU11I 561 ACATGT A^CATGT BsrFI 1004 ACCGGC [GA]^CCGG[CT] BstXI 728 CCAGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1004 ACCGGC [GA]^CCGG[CT] Cfr13I 747 GGACC G^G[ACGT]CC DpnII 1278 GATC ^GATC EcoRI 230 GAATTC G^AATTC EcoRV 357 GATATC GAT^ATC FauI 45 GCGGG CCCGC(4/6) MboI 1278 GATC ^GATC MslI 1201 CATCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 1071 CCGGG CC^[GC]GG NdeII 1278 GATC ^GATC NspI 561 ACATGT [GA]CATG^[CT] PflMI 831 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1269 TCATGA T^CATGA Sau3AI 1278 GATC ^GATC Sau96I 747 GGACC G^G[ACGT]CC SfcI 1215 CTATAG C^T[GA][CT]AG SpeI 945 ACTAGT A^CTAGT SspI 276 AATATT AAT^ATT StuI 543 AGGCCT AGG^CCT TaqI 719 TCGA T^CGA TthHB8I 719 TCGA T^CGA Van91I 831 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 203 CCATCTTTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 762 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 230 GAATTC [GA]^AATT[CT] 937 GAATTT ApoI 230 GAATTC [GA]^AATT[CT] 937 GAATTT BalI 14 TGGCCA TGG^CCA 921 TGGCCA BstF5I 349 CATCC GGATG(2/0) 600 CATCC BstNI 906 CCAGG CC^[AT]GG 1236 CCTGG Cac8I 624 GCGTGC GC[ACGT]^[ACGT]GC 708 GCAGGC EarI 301 GAAGAG CTCTTC(1/4) 313 GAAGAG EcoRII 906 CCAGG ^CC[AT]GG 1236 CCTGG EcoT14I 123 CCAAGG C^C[AT][AT]GG 352 CCTAGG FokI 349 CATCC GGATG(9/13) 600 CATCC HapII 1005 CCGG C^CGG 1071 CCGG HpaII 1005 CCGG C^CGG 1071 CCGG Ksp632I 301 GAAGAG CTCTTC(1/4) 313 GAAGAG MluNI 14 TGGCCA TGG^CCA 921 TGGCCA MscI 14 TGGCCA TGG^CCA 921 TGGCCA MspI 1005 CCGG C^CGG 1071 CCGG MvaI 906 CCAGG CC^[AT]GG 1236 CCTGG PleI 66 GACTC GAGTC(4/5) 1110 GAGTC SmlI 631 CTTGAG C^T[CT][GA]AG 1180 CTTGAG StyI 123 CCAAGG C^C[AT][AT]GG 352 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AspHI BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI DdeI EaeI Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboII MnlI MseI NlaIII NlaIV ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI