Restriction map for
pFN21AE4081
(Circular) (Six-base) MAPSORT of: pFN21AE4081.seq Check: 9350 from: 1 to: 5989
pFN21AE4081 5989 bp
With 182 enzymes: SgfI *
March 28, 2012 13:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 4052 5436 5704 1632
Size: 531 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 531 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 2566 4888 5865 1203
Size: 1363 2322 977 1327
Fragments arranged by size:
2322 1363 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 2028 3732 5304 2028
Size: 1704 1572 2713
Fragments arranged by size:
2713 1704 1572
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2235 2942 3298 3524 3676 5785 1493
Size: 742 707 356 226 152 2109 1697
Fragments arranged by size:
2109 1697 742 707 356 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2509 3314 1870
Size: 73 566 805 4545
Fragments arranged by size:
4545 805 566 73
AvrII C'CTAG_G
Cuts at: 2357 2357
Size: 5989
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3310 4765 618
Size: 325 217 627 1523 1455 1842
Fragments arranged by size:
1842 1523 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3308 1391
Size: 1917 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4600 136
Size: 107 122 71 631 605 2928 1525
Fragments arranged by size:
2928 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 4050 5206 1790
Size: 2260 1156 2573
Fragments arranged by size:
2573 2260 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3720 4678 442
Size: 416 53 83 399 24 2303 958 1753
Fragments arranged by size:
2303 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4649 1239
Size: 3410 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 3206 4114 4979 5277 2657
Size: 549 908 865 298 3369
Fragments arranged by size:
3369 908 865 549 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5764
Fragments arranged by size:
5764 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3129 1186
Size: 1943 4046
BseYI C'CCAG_C
Cuts at: 3150 5192 5688 3150
Size: 2042 496 3451
Fragments arranged by size:
3451 2042 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2472 2635 3109 4050 4135 5206 2472
Size: 163 474 941 85 1071 3255
Fragments arranged by size:
3255 1071 941 474 163 85
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2472 2635 3109 3308 4050 4135 5206
Size: 399 682 163 474 199 742 85 1071
Cuts at: 5206 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 682 474 399 199 163
85
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 3274 3878 5608 3274
Size: 604 1730 3655
Fragments arranged by size:
3655 1730 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3344 844
Size: 2500 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3009 4633 4820 1700
Size: 110 1199 1624 187 2869
Fragments arranged by size:
2869 1624 1199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstXI CCAn_nnnn'nTGG
Cuts at: 2740 2740
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 3319 3686 4071 4088 5540 5626 5638 5984
Size: 1347 367 385 17 1452 86 12 346
Cuts at: 5984 1972
Size: 1977
Fragments arranged by size:
1977 1452 1347 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 3230 3505 4299 4319 3230
Size: 275 794 20 4900
Fragments arranged by size:
4900 794 275 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2926 3011
Size: 54 1425 50 275 18 189 907 85
Cuts at: 3011 3436 4325 5954 8
Size: 425 889 1629 43
Fragments arranged by size:
1629 1425 907 889 425 275 189 85
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 2313 3919 1353
Size: 948 12 1606 3423
Fragments arranged by size:
3423 1606 948 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 4052 4649 5436 5704 1239
Size: 393 531 1889 597 787 268 1524
Fragments arranged by size:
1889 1524 787 597 531 393 268
EcoRI G'AATT_C
Cuts at: 2235 3298 3676 2235
Size: 1063 378 4548
Fragments arranged by size:
4548 1063 378
EcoRV GAT'ATC
Cuts at: 2364 2364
Size: 5989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2831 2831
Size: 5989
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 5136 1678
Size: 3458 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2999 3031 3269
Size: 32 135 32 749 32 646 32 238
Cuts at: 3269 3301 4630 4662 4704 4736 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 749 646 238 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3579 669
Size: 988 281 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2143 2730 3143 5103 5287 1305
Size: 838 587 413 1960 184 2007
Fragments arranged by size:
2007 1960 838 587 413 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 2928 10
Size: 54 1425 343 189 907 3071
Fragments arranged by size:
3071 1425 907 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3210 3947 4306 4465 518
Size: 648 2044 737 359 159 2042
Fragments arranged by size:
2044 2042 737 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3405 4082 5230 5475 1606
Size: 1799 677 1148 245 2120
Fragments arranged by size:
2120 1799 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5437
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2570 3347 4892 1132
Size: 97 1341 777 1545 2229
Fragments arranged by size:
2229 1545 1341 777 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 2566 4888 2566
Size: 2322 3667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 1108
Size: 1734 4255
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 2964 3559 2964
Size: 595 5394
PstI C_TGCA'G
Cuts at: 830 3341 830
Size: 2511 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 3220 3337 4472 5153 5344 826
Size: 219 2175 117 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 3185 4093 4994 5256 820
Size: 197 926 693 549 908 901 262 1553
Fragments arranged by size:
1553 926 908 901 693 549 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 2950 152
Size: 2798 3191
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 2283 3913 5
Size: 47 2231 1630 2081
Fragments arranged by size:
2231 2081 1630 47
StuI AGG'CCT
Cuts at: 1517 2550 1517
Size: 1033 4956
StyI C'CwwG_G
Cuts at: 513 1065 2128 2357 3428 513
Size: 552 1063 229 1071 3074
Fragments arranged by size:
3074 1071 1063 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4016 4033 4186 4371 4816 325
Size: 1255 2436 17 153 185 445 1498
Fragments arranged by size:
2436 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4235 5947 96
Size: 274 80 33 51 1443 2258 1712 138
Fragments arranged by size:
2258 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3160 3192 5949 5981 3160
Size: 32 2757 32 3168
Fragments arranged by size:
3168 2757 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2215
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 2771 4118 2771
Size: 1347 4642
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI