Restriction map for
pFN21AE5552
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# NO CUT ENZYMES ( 162 enzymes) #
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Acc65I AccI AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbsI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HindIII KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 4 GACGTC GACGT^C
AcyI 4 GACGTC G[GA]^CG[CT]C
AlwNI 188 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 226 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 585 GAGCAC G[AT]GC[AT]^C
BamHI 1153 GGATCC G^GATCC
BanII 675 GAGCCC G[GA]GC[CT]^C
BbvCI 718 GCTGAGG CCTCAGC(-5/-2)
BciVI 287 GTATCC GTATCC(6/5)
BcnI 875 CCCGG CC^[GC]GG
BsaHI 4 GACGTC G[GA]^CG[CT]C
BsiHKAI 585 GAGCAC G[AT]GC[AT]^C
Bsp1407I 886 TGTACA T^GTACA
BsrGI 886 TGTACA T^GTACA
CfoI 25 GCGC GCG^C
DsaI 1196 CCATGG C^C[GA][CT]GG
HaeII 24 GGCGCT [GA]GCGC^[CT]
HapII 876 CCGG C^CGG
HhaI 25 GCGC GCG^C
Hin1I 4 GACGTC G[GA]^CG[CT]C
HinP1I 25 GCGC G^CGC
HincII 321 GTTAAC GT[CT]^[GA]AC
HindII 321 GTTAAC GT[CT]^[GA]AC
HpaI 321 GTTAAC GTT^AAC
HpaII 876 CCGG C^CGG
MaeII 5 ACGT A^CGT
MslI 694 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 876 CCGG C^CGG
NciI 875 CCCGG CC^[GC]GG
NcoI 1196 CCATGG C^CATGG
PpuMI 204 AGGTCCT [GA]G^G[AT]CC[CT]
SmlI 1055 CTCAAG C^T[CT][GA]AG
SspBI 886 TGTACA T^GTACA
XmnI 226 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 872 GTAC GT^AC
887 GTAC
AvaII 125 GGTCC G^G[AT]CC
205 GGTCC
BbvI 612 GCAGC GCAGC(8/12)
879 GCAGC
BmyI 585 GAGCAC G[AGT]GC[ACT]^C
675 GAGCCC
BseRI 368 GAGGAG GAGGAG(10/8)
622 GAGGAG
BsmAI 1012 GAGAC GTCTC(1/5)
1053 GTCTC
BsmFI 514 GGGAC GGGAC(10/14)
126 GTCCC
Bsp1286I 585 GAGCAC G[AGT]GC[ACT]^C
675 GAGCCC
BstYI 327 AGATCC [GA]^GATC[CT]
1153 GGATCC
DraI 133 TTTAAA TTT^AAA
438 TTTAAA
DraII 204 AGGTCCT [GA]G^G[ACGT]CC[CT]
1101 AGGCCCT
EcoO109I 204 AGGTCCT [GA]G^G[ACGT]CC[CT]
1101 AGGCCCT
EcoT14I 331 CCAAGG C^C[AT][AT]GG
1196 CCATGG
Fnu4HI 612 GCAGC GC^[ACGT]GC
879 GCAGC
ItaI 612 GCAGC GC^[ACGT]GC
879 GCAGC
MflI 327 AGATCC [GA]^GATC[CT]
1153 GGATCC
MwoI 587 GCACAGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
709 GCTGTGGAAGC
RsaI 872 GTAC GT^AC
887 GTAC
StyI 331 CCAAGG C^C[AT][AT]GG
1196 CCATGG
TseI 612 GCAGC G^C[AT]GC
879 GCAGC
XhoII 327 AGATCC [GA]^GATC[CT]
1153 GGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI AlwI ApoI BpmI
BsaJI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EarI EcoRII
FokI HaeIII HinfI HphI Ksp632I
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I