(Circular) (Six-base) MAPSORT of: pFN21AE5552.seq Check: 7164 from: 1 to: 5956 pFN21AE5552 5956 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2013 3766 278 Size: 53 83 186 936 477 1753 2468 Fragments arranged by size: 2468 1753 936 477 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2175 2403 2796 3108 4019 5403 5671 1632 Size: 543 228 393 312 911 1384 268 1917 Fragments arranged by size: 1917 1384 911 543 393 312 268 228 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2179 2211 2179 Size: 32 5924 AlwNI CAG_nnn'CTG Cuts at: 2198 3699 5271 2198 Size: 1501 1572 2883 Fragments arranged by size: 2883 1572 1501 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2363 2476 2611 2805 3265 3491 3643 5752 Size: 870 113 135 194 460 226 152 2109 Cuts at: 5752 1493 Size: 1697 Fragments arranged by size: 2109 1697 870 460 226 194 152 135 113 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 3158 3286 3653 3158 Size: 128 367 5461 Fragments arranged by size: 5461 367 128 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3277 4732 618 Size: 325 217 627 1490 1455 1842 Fragments arranged by size: 1842 1490 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2684 3275 1391 Size: 1293 591 4072 Fragments arranged by size: 4072 1293 591 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BbvCI CC'TCA_GC Cuts at: 2724 2724 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2303 3848 5058 2303 Size: 1545 1210 3201 Fragments arranged by size: 3201 1545 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 4017 5173 1790 Size: 2227 1156 2573 Fragments arranged by size: 2573 2227 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2006 2130 2183 3198 4616 1239 Size: 767 124 53 1015 1418 2579 Fragments arranged by size: 2579 1418 1015 767 124 53 Bpu10I CC'TnA_GC Cuts at: 2199 2724 2199 Size: 525 5431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3045 4081 4946 5244 3045 Size: 1036 865 298 3757 Fragments arranged by size: 3757 1036 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 3763 4145 4780 Size: 53 83 186 936 477 1753 382 635 Cuts at: 4780 275 Size: 1451 Fragments arranged by size: 1753 1451 936 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2179 2209 1178 Size: 30 165 30 776 30 4925 Fragments arranged by size: 4925 776 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 2642 1186 Size: 1202 254 4500 Fragments arranged by size: 4500 1202 254 BseYI C'CCAG_C Cuts at: 5159 5655 5159 Size: 496 5460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2594 4017 4102 5173 2594 Size: 1423 85 1071 3377 Fragments arranged by size: 3377 1423 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2594 2684 3275 4017 4102 5173 1391 Size: 399 804 90 591 742 85 1071 2174 Fragments arranged by size: 2174 1071 804 742 591 399 90 85 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3311 844 Size: 2467 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 2891 96 Size: 2795 3161 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2332 3158 3286 3653 4038 4055 5507 5593 Size: 360 826 128 367 385 17 1452 86 Cuts at: 5593 5605 5951 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 826 385 367 360 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3201 513 Size: 552 939 1197 3268 Fragments arranged by size: 3268 1197 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 2140 2445 3260 2140 Size: 305 815 4836 Fragments arranged by size: 4836 815 305 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921 Size: 54 1425 50 275 18 1573 889 1629 Cuts at: 5921 8 Size: 43 Fragments arranged by size: 1629 1573 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2653 2892 2904 2913 2925 3009 3886 Size: 689 611 239 12 9 12 84 877 Cuts at: 3886 1353 Size: 3423 Fragments arranged by size: 3423 877 689 611 239 84 12 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2130 2175 2183 2403 2796 3108 Size: 393 374 124 45 8 220 393 312 Cuts at: 3108 3198 4019 4616 5403 5671 1239 Size: 90 821 597 787 268 1524 Fragments arranged by size: 1524 821 787 597 393 393 374 312 268 220 124 90 45 8 EcoO109I rG'GnC_Cy Cuts at: 2210 3107 2210 Size: 897 5059 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2033 5103 1678 Size: 355 3070 2531 Fragments arranged by size: 3070 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2943 2975 4597 4629 4671 Size: 32 135 32 1371 32 1622 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 1622 1371 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2328 3300 3546 669 Size: 988 281 390 972 246 3079 Fragments arranged by size: 3079 988 972 390 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 2328 3546 2328 Size: 1218 4738 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2703 3914 4273 4432 518 Size: 648 1537 1211 359 159 2042 Fragments arranged by size: 2042 1537 1211 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3372 4049 5197 5442 1606 Size: 1766 677 1148 245 2120 Fragments arranged by size: 2120 1766 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3201 513 Size: 552 939 1197 3268 Fragments arranged by size: 3268 1197 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PfoI T'CCnGG_A Cuts at: 2022 2076 2470 2022 Size: 54 394 5508 Fragments arranged by size: 5508 394 54 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2179 2211 4025 4057 5538 5570 2179 Size: 32 1814 32 1481 32 2565 Fragments arranged by size: 2565 1814 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2210 2210 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 3304 4439 5120 5311 826 Size: 219 2259 1135 681 191 1471 Fragments arranged by size: 2259 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3060 4060 4961 5223 820 Size: 197 926 1117 1000 901 262 1553 Fragments arranged by size: 1553 1117 1000 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2004 2336 3201 3395 513 Size: 552 939 332 865 194 3074 Fragments arranged by size: 3074 939 865 552 332 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2539 3983 4000 4153 4338 4783 325 Size: 1255 959 1444 17 153 185 445 1498 Fragments arranged by size: 1498 1444 1255 959 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2891 4202 5914 Size: 274 80 33 51 1443 914 1311 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1712 1443 1311 914 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 2235 4085 2235 Size: 1850 4106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2011 3764 276 Size: 53 83 186 936 477 1753 2468 Fragments arranged by size: 2468 1753 936 477 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI