################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 67 CGCG CG^CG AcyI 76 GACGCC G[GA]^CG[CT]C AluI 199 AGCT AG^CT AlwNI 144 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG BclI 86 TGATCA T^GATCA BcnI 92 CCGGG CC^[GC]GG BsaHI 76 GACGCC G[GA]^CG[CT]C BsaJI 92 CCGGGG C^C[ACGT][ACGT]GG BsmAI 194 GAGAC GTCTC(1/5) BsmFI 74 GGGAC GGGAC(10/14) BsrI 189 ACTGG ACTGG(1/-1) BstNI 268 CCAGG CC^[AT]GG BstUI 67 CGCG CG^CG BstXI 114 CCAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 87 GATC ^GATC EcoRII 268 CCAGG ^CC[AT]GG EcoT22I 244 ATGCAT ATGCA^T FauI 65 CCCGC CCCGC(4/6) FbaI 86 TGATCA T^GATCA HaeIII 276 GGCC GG^CC HapII 92 CCGG C^CGG HgaI 76 GACGC GACGC(5/10) Hin1I 76 GACGCC G[GA]^CG[CT]C HpaII 92 CCGG C^CGG HphI 89 TCACC GGTGA(8/7) MaeIII 101 GTGAC ^GT[ACGT]AC MboI 87 GATC ^GATC MseI 296 TTAA T^TAA MslI 174 CACACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 92 CCGG C^CGG MvaI 268 CCAGG CC^[AT]GG MvnI 67 CGCG CG^CG NciI 92 CCGGG CC^[GC]GG NdeII 87 GATC ^GATC NlaIV 5 GGAGCC GG[ACGT]^[ACGT]CC NsiI 244 ATGCAT ATGCA^T NspI 128 GCATGT [GA]CATG^[CT] PflMI 226 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 198 CAGCTG CAG^CTG Sau3AI 87 GATC ^GATC Tru9I 296 TTAA T^TAA Tsp45I 101 GTGAC ^GT[GC]AC TspRI 209 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 226 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BseRI 31 CTCCTC GAGGAG(10/8) 3 GAGGAG BssKI 92 CCGGG ^CC[ACGT]GG 268 CCAGG BstF5I 205 CATCC GGATG(2/0) 242 GGATG CfoI 17 GCGC GCG^C 68 GCGC FokI 205 CATCC GGATG(9/13) 242 GGATG HhaI 17 GCGC GCG^C 68 GCGC HinP1I 17 GCGC G^CGC 68 GCGC HinfI 109 GACTC G^A[ACGT]TC 213 GACTC MspA1I 49 CCGCTG C[AC]G^C[GT]G 198 CAGCTG NlaIII 129 CATG CATG^ 260 CATG PleI 109 GACTC GAGTC(4/5) 213 GACTC ScrFI 92 CCGGG CC^[ACGT]GG 268 CCAGG SfaNI 243 GATGC GCATC(5/9) 246 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI BbvI BsiYI BslI Fnu4HI ItaI MnlI MwoI TseI