################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 359 ACATGT A^C[GA][CT]GT Alw44I 416 GTGCAC G^TGCAC AocI 558 CCTCAGG CC^T[ACGT]AGG ApaI 234 GGGCCC GGGCC^C ApaLI 416 GTGCAC G^TGCAC Asp700I 477 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 416 GTGCAC G[AT]GC[AT]^C AvaII 452 GGACC G^G[AT]CC BalI 625 TGGCCA TGG^CCA BanII 234 GGGCCC G[GA]GC[CT]^C BclI 263 TGATCA T^GATCA BfaI 385 CTAG C^TAG BglI 108 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiHKAI 416 GTGCAC G[AT]GC[AT]^C BspHI 266 TCATGA T^CATGA BspLU11I 359 ACATGT A^CATGT BstBI 68 TTCGAA TT^CGAA BstXI 224 CCAACGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 140 AGATCC [GA]^GATC[CT] Bsu36I 558 CCTCAGG CC^T[ACGT]AGG DdeI 559 CTCAG C^T[ACGT]AG DraI 202 TTTAAA TTT^AAA DraII 234 GGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 382 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 529 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 326 CCACGG C^C[GA][CT]GG EaeI 625 TGGCCA [CT]^GGCC[GA] EarI 481 CTCTTC CTCTTC(1/4) Eco81I 558 CCTCAGG CC^T[ACGT]AGG EcoO109I 234 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 134 CCAAGG C^C[AT][AT]GG FbaI 263 TGATCA T^GATCA HinfI 270 GAGTC G^A[ACGT]TC HphI 315 GGTGA GGTGA(8/7) Ksp632I 481 CTCTTC CTCTTC(1/4) MaeI 385 CTAG C^TAG MaeII 227 ACGT A^CGT MaeIII 49 GTCAC ^GT[ACGT]AC MflI 140 AGATCC [GA]^GATC[CT] MluNI 625 TGGCCA TGG^CCA MscI 625 TGGCCA TGG^CCA MslI 261 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 531 CAGCTG C[AC]G^C[GT]G NspV 68 TTCGAA TT^CGAA PleI 270 GAGTC GAGTC(4/5) PstI 210 CTGCAG CTGCA^G PvuII 531 CAGCTG CAG^CTG RcaI 266 TCATGA T^CATGA SfcI 210 CTGCAG C^T[GA][CT]AG SfuI 68 TTCGAA TT^CGAA SphI 653 GCATGC GCATG^C StuI 297 AGGCCT AGG^CCT StyI 134 CCAAGG C^C[AT][AT]GG TaqI 69 TCGA T^CGA Tsp45I 49 GTCAC ^GT[GC]AC TthHB8I 69 TCGA T^CGA XhoII 140 AGATCC [GA]^GATC[CT] XmnI 477 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 221 CCGC CCGC(-3/-1) 258 CCGC AcsI 391 AAATTC [GA]^AATT[CT] 404 AAATTC AfaI 156 GTAC GT^AC 363 GTAC AlwI 141 GATCC GGATC(4/5) 445 GATCC ApoI 391 AAATTC [GA]^AATT[CT] 404 AAATTC BbsI 253 GAAGAC GAAGAC(2/6) 538 GTCTTC BcnI 409 CCGGG CC^[GC]GG 525 CCGGG BmyI 234 GGGCCC G[AGT]GC[ACT]^C 416 GTGCAC BpmI 368 CTGGAG CTGGAG(16/14) 637 CTGGAG BpuAI 253 GAAGAC GAAGAC(2/6) 538 GTCTTC Bsp1286I 234 GGGCCC G[AGT]GC[ACT]^C 416 GTGCAC BsrI 420 ACTGG ACTGG(1/-1) 516 ACTGG HapII 409 CCGG C^CGG 525 CCGG HpaII 409 CCGG C^CGG 525 CCGG MspI 409 CCGG C^CGG 525 CCGG NciI 409 CCGGG CC^[GC]GG 525 CCGGG NspI 359 ACATGT [GA]CATG^[CT] 653 GCATGC RsaI 156 GTAC GT^AC 363 GTAC Tsp509I 392 AATT ^AATT 405 AATT TspRI 213 CAGTG CA[GC]TG[ACGT][ACGT]^ 419 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI BbvI BsaJI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tru9I TseI