Restriction map for
pFN21AE3983
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BbvI BcgI BclI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstF5I
BstPI BstYI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI Fnu4HI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI ItaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluNI MroI MscI MspA1I
MunI MwoI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TseI
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 66 GTATAC GT^[AC][GT]AC
AccII 92 CGCG CG^CG
AcsI 253 GAATTT [GA]^AATT[CT]
AflII 213 CTTAAG C^TTAAG
AflIII 91 ACGCGT A^C[GA][CT]GT
ApoI 253 GAATTT [GA]^AATT[CT]
AspHI 667 GAGCAC G[AT]GC[AT]^C
AspI 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 295 GAGCCC G[GA]GC[CT]^C
BbsI 699 GAAGAC GAAGAC(2/6)
BfrI 213 CTTAAG C^TTAAG
BpuAI 699 GAAGAC GAAGAC(2/6)
BsaI 149 GAGACC GGTCTC(1/5)
BsiHKAI 667 GAGCAC G[AT]GC[AT]^C
BsiYI 576 CCACCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 576 CCACCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 74 CGTCTC CGTCTC(1/5)
BsmFI 131 GGGAC GGGAC(10/14)
BsrBI 643 GAGCGG CCGCTC(-3/-3)
Bst1107I 66 GTATAC GTA^TAC
BstUI 92 CGCG CG^CG
BstXI 398 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 66 GTATAC GTA^TAC
DsaI 338 CCATGG C^C[GA][CT]GG
EcoT14I 338 CCATGG C^C[AT][AT]GG
FauI 645 GCGGG CCCGC(4/6)
HaeIII 648 GGCC GG^CC
HgaI 90 GACGC GACGC(5/10)
HindIII 382 AAGCTT A^AGCTT
MaeII 73 ACGT A^CGT
MluI 91 ACGCGT A^CGCGT
MvnI 92 CGCG CG^CG
NcoI 338 CCATGG C^CATGG
NheI 690 GCTAGC G^CTAGC
PaeR7I 146 CTCGAG C^TCGAG
PflFI 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 576 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 684 CTGTAG C^T[GA][CT]AG
SmaI 623 CCCGGG CCC^GGG
StyI 338 CCATGG C^C[AT][AT]GG
Tsp45I 40 GTCAC ^GT[GC]AC
Tth111I 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 576 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 146 CTCGAG C^TCGAG
XmaCI 623 CCCGGG C^CCGGG
XmaI 623 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 615 CCGC CCGC(-3/-1)
645 GCGG
AfaI 374 GTAC GT^AC
393 GTAC
AlwI 319 GATCC GGATC(4/5)
612 GATCC
AlwNI 404 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
510 CAGAGACTG
AvaI 146 CTCGAG C^[CT]CG[GA]G
623 CCCGGG
BciVI 342 GGATAC GTATCC(6/5)
333 GTATCC
BmyI 295 GAGCCC G[AGT]GC[ACT]^C
667 GAGCAC
BsaJI 338 CCATGG C^C[ACGT][ACGT]GG
623 CCCGGG
BsoBI 146 CTCGAG C^[CT]CG[GA]G
623 CCCGGG
Bsp1286I 295 GAGCCC G[AGT]GC[ACT]^C
667 GAGCAC
BstNI 519 CCAGG CC^[AT]GG
555 CCAGG
Cac8I 645 GCGGGC GC[ACGT]^[ACGT]GC
690 GCTAGC
EcoRII 519 CCAGG ^CC[AT]GG
555 CCAGG
HinfI 103 GATTC G^A[ACGT]TC
303 GATTC
MaeIII 40 GTCAC ^GT[ACGT]AC
95 GTTAC
MboII 352 GAAGA GAAGA(8/7)
699 GAAGA
MslI 621 CACCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
634 CACATGAGTG
MvaI 519 CCAGG CC^[AT]GG
555 CCAGG
NlaIV 538 GGACCC GG[ACGT]^[ACGT]CC
572 GGGTCC
RsaI 374 GTAC GT^AC
393 GTAC
ScaI 373 AGTACT AGT^ACT
392 AGTACT
SfaNI 464 GCATC GCATC(5/9)
630 GCATC
TaqI 147 TCGA T^CGA
177 TCGA
TfiI 103 GATTC G^A[AT]TC
303 GATTC
TthHB8I 147 TCGA T^CGA
177 TCGA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI AvaII BcnI BfaI BsmAI
BsrI BssKI Cfr13I DdeI DpnII
HapII HpaII HphI MaeI MboI
MnlI MseI MspI NciI NdeII
NlaIII Sau3AI Sau96I ScrFI SmlI
Tru9I Tsp509I TspRI