Restriction map for
pFN21AE4152
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsaJI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I EcoO109I
EcoO65I EcoRV EcoT14I FauI FseI
FspI HgaI Hin1I KasI KpnI
KspI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 1625 TCCGGA T^CCGGA
AfaI 210 GTAC GT^AC
AspI 1369 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1136 GAGCTC G[GA]GC[CT]^C
BcnI 1155 CCCGG CC^[GC]GG
BfaI 886 CTAG C^TAG
BsaAI 639 CACGTA [CT]AC^GT[GA]
BsaBI 523 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1625 TCCGGA [AT]^CCGG[AT]
BseAI 1625 TCCGGA T^CCGGA
BsgI 959 GTGCAG GTGCAG(16/14)
BsmAI 1367 GAGAC GTCTC(1/5)
BsmFI 1638 GGGAC GGGAC(10/14)
BsmI 126 GCATTC GAATGC(1/-1)
BspEI 1625 TCCGGA T^CCGGA
BspMI 848 ACCTGC ACCTGC(4/8)
BstAPI 97 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 442 TTCGAA TT^CGAA
BstXI 854 CCATCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 996 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 837 GAATTC G^AATTC
HindIII 598 AAGCTT A^AGCTT
MaeI 886 CTAG C^TAG
MaeII 640 ACGT A^CGT
MamI 523 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1625 TCCGGA T^CCGGA
NciI 1155 CCCGG CC^[GC]GG
NdeI 1703 CATATG CA^TATG
NspI 102 ACATGC [GA]CATG^[CT]
NspV 442 TTCGAA TT^CGAA
PflFI 1369 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 320 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 316 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 210 GTAC GT^AC
SacI 1136 GAGCTC GAGCT^C
SfuI 442 TTCGAA TT^CGAA
StuI 734 AGGCCT AGG^CCT
Tth111I 1369 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 320 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 213 CCATTCCGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 785 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
982 CAGTGCCTG
AocI 109 CCTAAGG CC^T[ACGT]AGG
1179 CCTCAGG
Aor51HI 139 AGCGCT AGC^GCT
307 AGCGCT
Bsu36I 109 CCTAAGG CC^T[ACGT]AGG
1179 CCTCAGG
Cfr13I 876 GGGCC G^G[ACGT]CC
1226 GGGCC
EarI 458 CTCTTC CTCTTC(1/4)
1107 CTCTTC
Eco47III 139 AGCGCT AGC^GCT
307 AGCGCT
Eco81I 109 CCTAAGG CC^T[ACGT]AGG
1179 CCTCAGG
EcoT22I 188 ATGCAT ATGCA^T
1632 ATGCAT
HaeII 139 AGCGCT [GA]GCGC^[CT]
307 AGCGCT
HapII 1156 CCGG C^CGG
1626 CCGG
HincII 1159 GTTAAC GT[CT]^[GA]AC
1252 GTTAAC
HindII 1159 GTTAAC GT[CT]^[GA]AC
1252 GTTAAC
HpaI 1159 GTTAAC GTT^AAC
1252 GTTAAC
HpaII 1156 CCGG C^CGG
1626 CCGG
Ksp632I 458 CTCTTC CTCTTC(1/4)
1107 CTCTTC
MspA1I 467 CAGCTG C[AC]G^C[GT]G
866 CAGCTG
MspI 1156 CCGG C^CGG
1626 CCGG
NsiI 188 ATGCAT ATGCA^T
1632 ATGCAT
PstI 266 CTGCAG CTGCA^G
1245 CTGCAG
PvuII 467 CAGCTG CAG^CTG
866 CAGCTG
Sau96I 876 GGGCC G^G[ACGT]CC
1226 GGGCC
TaqI 443 TCGA T^CGA
1485 TCGA
TthHB8I 443 TCGA T^CGA
1485 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BbsI BbvI BclI BmyI
BpmI BpuAI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI CfoI DdeI DpnII
EcoRII FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII