(Circular) (Six-base) MAPSORT of: pFN21AE4152.seq Check: 8887 from: 1 to: 6466 pFN21AE4152 6466 bp With 182 enzymes: SgfI * March 28, 2012 13:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2862 2862 Size: 6466 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4276 278 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3809 1937 Size: 1872 4594 Acc65I G'GTAC_C Cuts at: 3787 3787 Size: 6466 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 3099 4529 5913 6181 1632 Size: 669 798 1430 1384 268 1917 Fragments arranged by size: 1917 1430 1384 798 669 268 AfeI AGC'GCT Cuts at: 2146 2314 2146 Size: 168 6298 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6269 AflIII A'CryG_T Cuts at: 1203 5365 6342 1203 Size: 4162 977 1327 Fragments arranged by size: 4162 1327 977 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 5195 5195 Size: 6466 AleI CACnn'nnGTG Cuts at: 1166 2352 1166 Size: 1186 5280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2286 1184 Size: 1102 5364 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2988 3020 3471 3503 2988 Size: 32 451 32 5951 Fragments arranged by size: 5951 451 32 32 AlwNI CAG_nnn'CTG Cuts at: 2795 2992 4209 5781 2795 Size: 197 1217 1572 3480 Fragments arranged by size: 3480 1572 1217 197 ApaLI G'TGCA_C Cuts at: 4523 5679 4523 Size: 1156 5310 ApoI r'AATT_y Cuts at: 1493 2292 2443 2842 3747 3775 4001 4153 6262 Size: 799 151 399 905 28 226 152 2109 Cuts at: 6262 1493 Size: 1697 Fragments arranged by size: 2109 1697 905 799 399 226 152 151 28 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 3791 1870 Size: 73 1848 4545 Fragments arranged by size: 4545 1848 73 BamHI G'GATC_C Cuts at: 3796 4163 3796 Size: 367 6099 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3787 5242 618 Size: 325 217 627 2000 1455 1842 Fragments arranged by size: 2000 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3145 3785 1391 Size: 1754 640 4072 Fragments arranged by size: 4072 1754 640 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2034 2046 3661 928 Size: 971 135 12 1615 3733 Fragments arranged by size: 3733 1615 971 135 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4657 4691 1903 Size: 34 2720 34 3678 Fragments arranged by size: 3678 2720 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4358 5568 4358 Size: 1210 5256 BclI T'GATC_A Cuts at: 1548 2511 3207 3511 1548 Size: 963 696 304 4503 Fragments arranged by size: 4503 963 696 304 BfrBI ATG'CAT Cuts at: 2195 3639 2195 Size: 1444 5022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5077 136 Size: 107 122 71 631 605 3405 1525 Fragments arranged by size: 3405 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6461 6461 Size: 6466 BlpI GC'TnA_GC Cuts at: 3883 3883 Size: 6466 Bme1580I G_kGCm'C Cuts at: 1790 2626 4527 5683 1790 Size: 836 1901 1156 2573 Fragments arranged by size: 2573 1901 1156 836 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2319 2591 4197 Size: 416 53 83 399 24 902 272 1606 Cuts at: 4197 5155 442 Size: 958 1753 Fragments arranged by size: 1753 1606 958 902 416 399 272 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6466 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2577 2577 Size: 6466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2831 3158 3159 5126 1239 Size: 1592 327 1 1967 2579 Fragments arranged by size: 2579 1967 1592 327 1 Bpu10I CC'TnA_GC Cuts at: 2309 3221 3314 2309 Size: 912 93 5461 Fragments arranged by size: 5461 912 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4591 5456 5754 4591 Size: 865 298 5303 Fragments arranged by size: 5303 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5129 882 Size: 617 3630 2219 Fragments arranged by size: 3630 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2646 4257 493 Size: 711 1442 1611 2702 Fragments arranged by size: 2702 1611 1442 711 BsaBI GATnn'nnATC Cuts at: 2532 6460 2532 Size: 3928 2538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4273 4655 5290 275 Size: 53 83 186 936 2740 382 635 1451 Fragments arranged by size: 2740 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3630 4899 5297 5571 5718 6129 3630 Size: 1269 398 274 147 411 3967 Fragments arranged by size: 3967 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2514 2544 3363 3393 3584 Size: 30 165 30 1111 30 819 30 191 Cuts at: 3584 3614 1178 Size: 30 4030 Fragments arranged by size: 4030 1111 819 191 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2451 2649 2990 1186 Size: 1265 198 341 4662 Fragments arranged by size: 4662 1265 341 198 BseYI C'CCAG_C Cuts at: 2052 5669 6165 2052 Size: 3617 496 2353 Fragments arranged by size: 3617 2353 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2985 1163 Size: 573 1249 4644 Fragments arranged by size: 4644 1249 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3916 4677 5705 1540 Size: 275 186 1915 761 1028 2301 Fragments arranged by size: 2301 1915 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2645 2980 3145 3549 4527 4612 5683 2645 Size: 335 165 404 978 85 1071 3428 Fragments arranged by size: 3428 1071 978 404 335 165 85 BsmI GAATG_Cn' Cuts at: 2131 3977 4070 2131 Size: 1846 93 4527 Fragments arranged by size: 4527 1846 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2626 2645 2980 3145 3549 3785 4527 Size: 399 836 19 335 165 404 236 742 Cuts at: 4527 4612 5683 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 836 742 404 399 335 236 165 85 19 BspEI T'CCGG_A Cuts at: 3630 6129 3630 Size: 2499 3967 BspHI T'CATG_A Cuts at: 4355 6085 4355 Size: 1730 4736 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2862 3821 844 Size: 2018 959 3489 Fragments arranged by size: 3489 2018 959 BsrBI CCG'CTC Cuts at: 1394 1823 4353 1394 Size: 429 2530 3507 Fragments arranged by size: 3507 2530 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4961 5135 65 Size: 1183 3713 174 1396 Fragments arranged by size: 3713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5110 5297 1700 Size: 110 3300 187 2869 Fragments arranged by size: 3300 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6466 BssSI C'ACGA_G Cuts at: 4526 5538 4526 Size: 1012 5454 BstAPI GCAn_nnn'nTGC Cuts at: 794 2108 794 Size: 1314 5152 BstBI TT'CG_AA Cuts at: 2448 5270 2448 Size: 2822 3644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6466 BstXI CCAn_nnnn'nTGG Cuts at: 2866 2866 Size: 6466 BstYI r'GATC_y Cuts at: 1972 2920 3522 3561 3627 3796 4163 4548 4565 Size: 948 602 39 66 169 367 385 17 Cuts at: 4565 6017 6103 6115 6461 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 948 602 385 367 346 169 86 66 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2115 3185 2115 Size: 1070 5396 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4975 Fragments arranged by size: 4975 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2037 3364 487 Size: 683 56 811 1327 3589 Fragments arranged by size: 3589 1327 811 683 56 BtsI GCAGTG_nn' Cuts at: 2280 3281 3982 4776 4796 2280 Size: 1001 701 794 20 3950 Fragments arranged by size: 3950 1001 794 701 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2352 2387 529 Size: 35 1788 35 4608 Fragments arranged by size: 4608 1788 35 35 DraI TTT'AAA Cuts at: 3770 3770 Size: 6466 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5473 809 Size: 507 4157 1802 Fragments arranged by size: 4157 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3913 4802 6431 Size: 54 1425 50 275 18 2083 889 1629 Cuts at: 6431 8 Size: 43 Fragments arranged by size: 2083 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3913 1537 Size: 275 2101 4090 Fragments arranged by size: 4090 2101 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 3118 4396 1353 Size: 1116 649 1278 3423 Fragments arranged by size: 3423 1278 1116 649 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5053 5427 5573 387 Size: 1064 138 3464 374 146 1280 Fragments arranged by size: 3464 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3143 3143 Size: 6466 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2831 3099 3158 3159 4529 5126 Size: 393 669 530 268 59 1 1370 597 Cuts at: 5126 5913 6181 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1370 787 669 597 530 393 268 268 59 1 EcoNI CCTnn'n_nnAGG Cuts at: 3005 3005 Size: 6466 EcoRI G'AATT_C Cuts at: 2842 3775 4153 2842 Size: 933 378 5155 Fragments arranged by size: 5155 933 378 FspI TGC'GCA Cuts at: 4972 4972 Size: 6466 HaeII r_GCGC'y Cuts at: 1678 2148 2316 5613 1678 Size: 470 168 3297 2531 Fragments arranged by size: 3297 2531 470 168 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2514 2546 3363 Size: 32 135 32 611 32 299 32 817 Cuts at: 3363 3395 5107 5139 5181 5213 1373 Size: 32 1712 32 42 32 2626 Fragments arranged by size: 2626 1712 817 611 299 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3166 3259 3810 4056 669 Size: 988 281 1228 93 551 246 3079 Fragments arranged by size: 3079 1228 988 551 281 246 93 HindIII A'AGCT_T Cuts at: 748 2603 748 Size: 1855 4611 HpaI GTT'AAC Cuts at: 3166 3259 4056 3166 Size: 93 797 5576 Fragments arranged by size: 5576 797 93 KpnI G_GTAC'C Cuts at: 3791 3791 Size: 6466 MfeI C'AATT_G Cuts at: 4065 4065 Size: 6466 MluI A'CGCG_T Cuts at: 6342 6342 Size: 6466 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5580 5764 1305 Size: 4275 184 2007 Fragments arranged by size: 4275 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4644 Fragments arranged by size: 4644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2352 2702 2864 3060 4424 4783 4942 Size: 648 1186 350 162 196 1364 359 159 Cuts at: 4942 518 Size: 2042 Fragments arranged by size: 2042 1364 1186 648 359 350 196 162 159 MspA1I CmG'CkG Cuts at: 1606 2474 2873 3882 4559 5707 5952 1606 Size: 868 399 1009 677 1148 245 2120 Fragments arranged by size: 2120 1148 1009 868 677 399 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4975 Fragments arranged by size: 4975 939 552 NdeI CA'TA_TG Cuts at: 387 3709 387 Size: 3322 3144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6466 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NsiI A_TGCA'T Cuts at: 2197 3641 2197 Size: 1444 5022 NspI r_CATG'y Cuts at: 1132 1229 2111 3824 5369 1132 Size: 97 882 1713 1545 2229 Fragments arranged by size: 2229 1713 1545 882 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6466 PciI A'CATG_T Cuts at: 5365 5365 Size: 6466 PflMI CCAn_nnn'nTGG Cuts at: 1108 2331 1108 Size: 1223 5243 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4535 4567 6048 6080 4535 Size: 32 1481 32 4921 Fragments arranged by size: 4921 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2325 1613 Size: 712 5754 PsiI TTA'TAA Cuts at: 2088 4036 2088 Size: 1948 4518 PstI C_TGCA'G Cuts at: 830 2275 3254 3818 830 Size: 1445 979 564 3478 Fragments arranged by size: 3478 1445 979 564 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 PvuII CAG'CTG Cuts at: 2474 2873 2474 Size: 399 6067 SacI G_AGCT'C Cuts at: 3145 3145 Size: 6466 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SbfI CC_TGCA'GG Cuts at: 3818 3818 Size: 6466 ScaI AGT'ACT Cuts at: 4714 6426 4714 Size: 1712 4754 SfcI C'TryA_G Cuts at: 826 1045 2271 3250 3414 3814 4949 5630 5821 Size: 219 1226 979 164 400 1135 681 191 Cuts at: 5821 826 Size: 1471 Fragments arranged by size: 1471 1226 1135 979 681 400 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 3793 1872 Size: 1921 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4570 5471 5733 820 Size: 197 926 2627 901 262 1553 Fragments arranged by size: 2627 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SspI AAT'ATT Cuts at: 5 52 4390 5 Size: 47 4338 2081 Fragments arranged by size: 4338 2081 47 StuI AGG'CCT Cuts at: 1517 2741 1517 Size: 1224 5242 StyI C'CwwG_G Cuts at: 513 1065 2004 3905 513 Size: 552 939 1901 3074 Fragments arranged by size: 3074 1901 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 4493 4510 4663 4848 5293 325 Size: 1255 486 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4712 6424 96 Size: 274 80 33 51 1443 2735 1712 138 Fragments arranged by size: 2735 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3006 3038 6426 6458 3006 Size: 32 3388 32 3014 Fragments arranged by size: 3388 3014 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3377 1316 Size: 2061 4405 XbaI T'CTAG_A Cuts at: 3802 3802 Size: 6466 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2225 2225 Size: 6466 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6466 XmaI C'CCGG_G Cuts at: 1870 3791 1870 Size: 1921 4545 XmnI GAAnn'nnTTC Cuts at: 4595 4595 Size: 6466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4274 276 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI