Restriction map for
pFN21AE4152
(Linear) (Six-base) MAPSORT of: KIEE4152.seq Check: 6272 from: 1 to: 1761
KIEE4152.orf
With 182 enzymes: SgfI *
March 22, 2012 17:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 857 1761
Size: 857 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1094 1761
Size: 296 798 667
Fragments arranged by size:
798 667 296
AfeI AGC'GCT
Cuts at: 0 141 309 1761
Size: 141 168 1452
Fragments arranged by size:
1452 168 141
AleI CACnn'nnGTG
Cuts at: 0 347 1761
Size: 347 1414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 1761
Size: 281 1480
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1466 1498 1761
Size: 983 32 451 32 263
Fragments arranged by size:
983 451 263 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 790 987 1761
Size: 790 197 774
Fragments arranged by size:
790 774 197
ApoI r'AATT_y
Cuts at: 0 287 438 837 1742 1761
Size: 287 151 399 905 19
Fragments arranged by size:
905 399 287 151 19
BanII G_rGCy'C
Cuts at: 0 1140 1761
Size: 1140 621
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 41 1656 1761
Size: 29 12 1615 105
Fragments arranged by size:
1615 105 29 12
BclI T'GATC_A
Cuts at: 0 506 1202 1506 1761
Size: 506 696 304 255
Fragments arranged by size:
696 506 304 255
BfrBI ATG'CAT
Cuts at: 0 190 1634 1761
Size: 190 1444 127
Fragments arranged by size:
1444 190 127
Bme1580I G_kGCm'C
Cuts at: 0 621 1761
Size: 621 1140
BmrI ACTGGGnnnn_n'
Cuts at: 0 314 586 1761
Size: 314 272 1175
Fragments arranged by size:
1175 314 272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 572 1761
Size: 572 1189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1153 1154 1761
Size: 826 327 1 607
Fragments arranged by size:
826 607 327 1
Bpu10I CC'TnA_GC
Cuts at: 0 304 1216 1309 1761
Size: 304 912 93 452
Fragments arranged by size:
912 452 304 93
BsaAI yAC'GTr
Cuts at: 0 641 1761
Size: 641 1120
BsaBI GATnn'nnATC
Cuts at: 0 527 1761
Size: 527 1234
BsaWI w'CCGG_w
Cuts at: 0 1625 1761
Size: 1625 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 509 539 1358 1388 1579 1609 1761
Size: 509 30 819 30 191 30 152
Fragments arranged by size:
819 509 191 152 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 446 644 985 1761
Size: 446 198 341 776
Fragments arranged by size:
776 446 341 198
BseYI C'CCAG_C
Cuts at: 0 47 1761
Size: 47 1714
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1761
Size: 980 781
BsiHKAI G_wGCw'C
Cuts at: 0 640 975 1140 1544 1761
Size: 640 335 165 404 217
Fragments arranged by size:
640 404 335 217 165
BsmI GAATG_Cn'
Cuts at: 0 126 1761
Size: 126 1635
Bsp1286I G_dGCh'C
Cuts at: 0 621 640 975 1140 1544 1761
Size: 621 19 335 165 404 217
Fragments arranged by size:
621 404 335 217 165 19
BspEI T'CCGG_A
Cuts at: 0 1625 1761
Size: 1625 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 857 1761
Size: 857 904
BstAPI GCAn_nnn'nTGC
Cuts at: 0 103 1761
Size: 103 1658
BstBI TT'CG_AA
Cuts at: 0 443 1761
Size: 443 1318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 861 1761
Size: 861 900
BstYI r'GATC_y
Cuts at: 0 915 1517 1556 1622 1761
Size: 915 602 39 66 139
Fragments arranged by size:
915 602 139 66 39
Bsu36I CC'TnA_GG
Cuts at: 0 110 1180 1761
Size: 110 1070 581
Fragments arranged by size:
1070 581 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 32 1359 1761
Size: 32 1327 402
Fragments arranged by size:
1327 402 32
BtsI GCAGTG_nn'
Cuts at: 0 275 1276 1761
Size: 275 1001 485
Fragments arranged by size:
1001 485 275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 347 382 1761
Size: 347 35 1379
Fragments arranged by size:
1379 347 35
EarI CTCTTCn'nnn_
Cuts at: 0 464 1113 1761
Size: 464 649 648
Fragments arranged by size:
649 648 464
EcoICRI GAG'CTC
Cuts at: 0 1138 1761
Size: 1138 623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 826 1094 1153 1154 1761
Size: 296 530 268 59 1 607
Fragments arranged by size:
607 530 296 268 59 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1000 1761
Size: 1000 761
EcoRI G'AATT_C
Cuts at: 0 837 1761
Size: 837 924
HaeII r_GCGC'y
Cuts at: 0 143 311 1761
Size: 143 168 1450
Fragments arranged by size:
1450 168 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 509 541 1358 1390 1761
Size: 178 32 299 32 817 32 371
Fragments arranged by size:
817 371 299 178 32 32 32
HincII GTy'rAC
Cuts at: 0 1161 1254 1761
Size: 1161 93 507
Fragments arranged by size:
1161 507 93
HindIII A'AGCT_T
Cuts at: 0 598 1761
Size: 598 1163
HpaI GTT'AAC
Cuts at: 0 1161 1254 1761
Size: 1161 93 507
Fragments arranged by size:
1161 507 93
MslI CAynn'nnrTG
Cuts at: 0 347 697 859 1055 1761
Size: 347 350 162 196 706
Fragments arranged by size:
706 350 347 196 162
MspA1I CmG'CkG
Cuts at: 0 469 868 1761
Size: 469 399 893
Fragments arranged by size:
893 469 399
NdeI CA'TA_TG
Cuts at: 0 1704 1761
Size: 1704 57
NsiI A_TGCA'T
Cuts at: 0 192 1636 1761
Size: 192 1444 125
Fragments arranged by size:
1444 192 125
NspI r_CATG'y
Cuts at: 0 106 1761
Size: 106 1655
PflMI CCAn_nnn'nTGG
Cuts at: 0 326 1761
Size: 326 1435
PshAI GACnn'nnGTC
Cuts at: 0 320 1761
Size: 320 1441
PsiI TTA'TAA
Cuts at: 0 83 1761
Size: 83 1678
PstI C_TGCA'G
Cuts at: 0 270 1249 1761
Size: 270 979 512
Fragments arranged by size:
979 512 270
PvuII CAG'CTG
Cuts at: 0 469 868 1761
Size: 469 399 893
Fragments arranged by size:
893 469 399
SacI G_AGCT'C
Cuts at: 0 1140 1761
Size: 1140 621
SfcI C'TryA_G
Cuts at: 0 266 1245 1409 1761
Size: 266 979 164 352
Fragments arranged by size:
979 352 266 164
StuI AGG'CCT
Cuts at: 0 736 1761
Size: 736 1025
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 1761
Size: 61 1700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1761
Size: 1001 32 728
Fragments arranged by size:
1001 728 32
Tth111I GACn'n_nGTC
Cuts at: 0 1372 1761
Size: 1372 389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 220 1761
Size: 220 1541
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AloI AlwNI ApoI
BanII BbsI BclI BfrBI Bme1580I BmrI BplI BpmI
Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspMI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI CspCI EarI EcoICRI Eco57MI
EcoNI EcoRI HaeII Hin4I HincII HindIII HpaI MslI
MspA1I NdeI NsiI NspI PflMI PshAI PsiI PstI
PvuII SacI SfcI StuI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BglI BglII
BlpI BmgBI BmtI BpuEI BsaI BsaHI BsiEI BsiWI
BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstEII BstZ17I BtgI ClaI DraI DraIII DrdI EaeI
EagI EciI EcoO109I EcoRV FalI FseI FspI FspAI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI XbaI
XhoI XmaI XmnI ZraI