################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccI AclI AcsI AflII AflIII Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AvrII BamHI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BspDI BspLU11I Bst1107I BstBI BstZ17I CelII ClaI DraI DrdI Eco47III EcoRI EcoRV FbaI FseI HincII HindII HpaI KspI MamI MfeI MluI MunI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 2890 GACGTC GACGT^C Acc65I 79 GGTACC G^GTACC AccIII 2108 TCCGGA T^CCGGA AgeI 1561 ACCGGT A^CCGGT AhdI 1182 GACTCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 709 CCTGAGG CC^T[ACGT]AGG Asp718I 79 GGTACC G^GTACC AspEI 1182 GACTCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2821 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 573 CCCGGG C^[CT]CG[GA]G BbeI 705 GGCGCC GGCGC^C BseAI 2108 TCCGGA T^CCGGA BsmBI 1975 CGTCTC CGTCTC(1/5) BsmI 897 GAATGC GAATGC(1/-1) BsoBI 573 CCCGGG C^[CT]CG[GA]G Bsp1407I 2660 TGTACA T^GTACA BspEI 2108 TCCGGA T^CCGGA BspHI 404 TCATGA T^CATGA BsrGI 2660 TGTACA T^GTACA BssHII 2841 GCGCGC G^CGCGC BssSI 1609 CACGAG CACGAG(-5/-1) BstEII 1452 GGTGACC G^GT[ACGT]ACC BstPI 1452 GGTGACC G^GT[ACGT]ACC BstYI 1431 GGATCT [GA]^GATC[CT] Bsu36I 709 CCTGAGG CC^T[ACGT]AGG CpoI 2819 CGGACCG CG^G[AT]CCG DraIII 179 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1182 GACTCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 376 GAAGAG CTCTTC(1/4) Eco81I 709 CCTGAGG CC^T[ACGT]AGG EcoO65I 1452 GGTGACC G^GT[ACGT]ACC EcoT22I 966 ATGCAT ATGCA^T HindIII 1012 AAGCTT A^AGCTT KasI 705 GGCGCC G^GCGCC KpnI 79 GGTACC GGTAC^C Ksp632I 376 GAAGAG CTCTTC(1/4) MflI 1431 GGATCT [GA]^GATC[CT] MroI 2108 TCCGGA T^CCGGA NaeI 490 GCCGGC GCC^GGC NarI 705 GGCGCC GG^CGCC NdeI 1834 CATATG CA^TATG NgoMIV 490 GCCGGC G^CCGGC NsiI 966 ATGCAT ATGCA^T PflFI 2821 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 117 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1561 ACCGGT A^CCGGT RcaI 404 TCATGA T^CATGA RsrII 2819 CGGACCG CG^G[AT]CCG SapI 376 GAAGAGC GCTCTTC(1/4) SbfI 548 CCTGCAGG CCTGCA^GG SexAI 1448 ACCTGGT A^CC[AT]GGT SfoI 705 GGCGCC GGC^GCC SmaI 573 CCCGGG CCC^GGG Sse8387I 548 CCTGCAGG CCTGCA^GG SspBI 2660 TGTACA T^GTACA TfiI 238 GATTC G^A[AT]TC Tth111I 2821 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 117 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1431 GGATCT [GA]^GATC[CT] XmaCI 573 CCCGGG C^CCGGG XmaI 573 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 2731 CGCG CG^CG 2842 CGCG AcyI 705 GGCGCC G[GA]^CG[CT]C 2890 GACGTC AlwI 2907 GATCC GGATC(4/5) 1431 GGATC BalI 59 TGGCCA TGG^CCA 719 TGGCCA BbsI 1390 GAAGAC GAAGAC(2/6) 2623 GTCTTC BpuAI 1390 GAAGAC GAAGAC(2/6) 2623 GTCTTC BsaAI 1255 TACGTG [CT]AC^GT[GA] 2938 TACGTG BsaHI 705 GGCGCC G[GA]^CG[CT]C 2890 GACGTC BseRI 2977 CTCCTC GAGGAG(10/8) 2686 GAGGAG BsiEI 2922 CGGCCG CG[GA][CT]^CG 3089 CGGCCG BsrDI 188 GCAATG GCAATG(2/0) 1820 GCAATG BstUI 2731 CGCG CG^CG 2842 CGCG EagI 2922 CGGCCG C^GGCCG 3089 CGGCCG EclXI 2922 CGGCCG C^GGCCG 3089 CGGCCG Eco52I 2922 CGGCCG C^GGCCG 3089 CGGCCG Hin1I 705 GGCGCC G[GA]^CG[CT]C 2890 GACGTC MluNI 59 TGGCCA TGG^CCA 719 TGGCCA MscI 59 TGGCCA TGG^CCA 719 TGGCCA MseI 1016 TTAA T^TAA 2003 TTAA MvnI 2731 CGCG CG^CG 2842 CGCG NcoI 170 CCATGG C^CATGG 1307 CCATGG PleI 475 GACTC GAGTC(4/5) 1182 GACTC SacI 106 GAGCTC GAGCT^C 2185 GAGCTC SphI 498 GCATGC GCATG^C 1679 GCATGC Tru9I 1016 TTAA T^TAA 2003 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII AviII BanI BanII BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI