(Circular) (Six-base) MAPSORT of: pFN21AE3348.seq Check: 2844 from: 1 to: 7801 pFN21AE3348 7801 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2268 3504 3565 3732 2268 Size: 1236 61 167 6337 Fragments arranged by size: 6337 1236 167 61 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4899 5611 278 Size: 53 83 186 936 3363 712 2468 Fragments arranged by size: 3363 2468 936 712 186 83 53 AccI GT'mk_AC Cuts at: 1937 5144 1937 Size: 3207 4594 Acc65I G'GTAC_C Cuts at: 2084 5122 2084 Size: 3038 4763 AclI AA'CG_TT Cuts at: 1563 5928 6301 1563 Size: 4365 373 3063 Fragments arranged by size: 4365 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2827 3122 3220 3385 3414 3691 3802 Size: 542 653 295 98 165 29 277 111 Cuts at: 3802 4091 5864 7248 7516 1632 Size: 289 1773 1384 268 1917 Fragments arranged by size: 1917 1773 1384 653 542 295 289 277 268 165 111 98 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7604 AflIII A'CryG_T Cuts at: 1203 6700 7677 1203 Size: 5497 977 1327 Fragments arranged by size: 5497 1327 977 AgeI A'CCGG_T Cuts at: 3566 6632 3566 Size: 3066 4735 AhdI GACnn_n'nnGTC Cuts at: 3192 6530 3192 Size: 3338 4463 AleI CACnn'nnGTG Cuts at: 1166 2642 4518 1166 Size: 1476 1876 4449 Fragments arranged by size: 4449 1876 1476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2042 1184 Size: 858 6943 AlwNI CAG_nnn'CTG Cuts at: 2566 3190 3505 3556 3976 4420 4876 5019 5544 Size: 624 315 51 420 444 456 143 525 Cuts at: 5544 7116 2566 Size: 1572 3251 Fragments arranged by size: 3251 1572 624 525 456 444 420 315 143 51 ApaI G_GGCC'C Cuts at: 3811 4245 5026 3811 Size: 434 781 6586 Fragments arranged by size: 6586 781 434 ApaLI G'TGCA_C Cuts at: 5858 7014 5858 Size: 1156 6645 ApoI r'AATT_y Cuts at: 1493 5110 5336 5488 7597 1493 Size: 3617 226 152 2109 1697 Fragments arranged by size: 3617 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6355 160 Size: 872 5323 1606 Fragments arranged by size: 5323 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 AvaI C'yCGr_G Cuts at: 1870 1943 2578 5126 1870 Size: 73 635 2548 4545 Fragments arranged by size: 4545 2548 635 73 BamHI G'GATC_C Cuts at: 5131 5498 5131 Size: 367 7434 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2582 2644 2710 4103 Size: 325 217 627 297 498 62 66 1393 Cuts at: 4103 5122 6577 618 Size: 1019 1455 1842 Fragments arranged by size: 1842 1455 1393 1019 627 498 325 297 217 66 62 BanII G_rGCy'C Cuts at: 1391 2115 2352 3811 4194 4245 4922 5026 5120 Size: 724 237 1459 383 51 677 104 94 Cuts at: 5120 1391 Size: 4072 Fragments arranged by size: 4072 1459 724 677 383 237 104 94 51 BbeI G_GCGC'C Cuts at: 2714 2714 Size: 7801 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3402 4621 928 Size: 971 1503 1219 4108 Fragments arranged by size: 4108 1503 1219 971 BbvCI CC'TCA_GC Cuts at: 2312 2453 3659 3743 4400 2312 Size: 141 1206 84 657 5713 Fragments arranged by size: 5713 1206 657 141 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 4240 4274 4879 4913 5992 Size: 34 2192 34 77 34 605 34 1079 Cuts at: 5992 6026 1903 Size: 34 3678 Fragments arranged by size: 3678 2192 1079 605 77 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5693 6903 5693 Size: 1210 6591 BclI T'GATC_A Cuts at: 1548 1548 Size: 7801 BfrBI ATG'CAT Cuts at: 2973 2973 Size: 7801 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2496 4304 4926 Size: 107 122 71 631 605 824 1808 622 Cuts at: 4926 6412 136 Size: 1486 1525 Fragments arranged by size: 1808 1525 1486 824 631 622 605 122 107 71 BglII A'GATC_T Cuts at: 7796 7796 Size: 7801 BlpI GC'TnA_GC Cuts at: 5218 5218 Size: 7801 Bme1580I G_kGCm'C Cuts at: 1790 2166 2373 2587 2649 2810 3811 4245 4258 Size: 376 207 214 62 161 1001 434 13 Cuts at: 4258 4350 4503 4714 5026 5862 7018 1790 Size: 92 153 211 312 836 1156 2573 Fragments arranged by size: 2573 1156 1001 836 434 376 312 214 211 207 161 153 92 62 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7801 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3368 3544 3842 Size: 416 53 83 399 24 1951 176 298 Cuts at: 3842 4669 4995 5532 6490 442 Size: 827 326 537 958 1753 Fragments arranged by size: 1951 1753 958 827 537 416 399 326 298 176 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7801 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4462 4462 Size: 7801 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2588 2690 3167 3665 4349 4376 4709 Size: 860 489 102 477 498 684 27 333 Cuts at: 4709 4748 4901 6461 1239 Size: 39 153 1560 2579 Fragments arranged by size: 2579 1560 860 684 498 489 477 333 153 102 39 27 Bpu10I CC'TnA_GC Cuts at: 2312 2393 2453 2852 3512 3659 3743 3884 4199 Size: 81 60 399 660 147 84 141 315 Cuts at: 4199 4400 4916 2312 Size: 201 516 5197 Fragments arranged by size: 5197 660 516 399 315 201 147 141 84 81 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2270 2606 4199 5926 6791 7089 2270 Size: 336 1593 1727 865 298 2982 Fragments arranged by size: 2982 1727 1593 865 336 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3140 3849 4844 6464 882 Size: 617 1641 709 995 1620 2219 Fragments arranged by size: 2219 1641 1620 995 709 617 BsaAI yAC'GTr Cuts at: 493 1204 3262 4945 5592 493 Size: 711 2058 1683 647 2702 Fragments arranged by size: 2702 2058 1683 711 647 BsaBI GATnn'nnATC Cuts at: 7795 7795 Size: 7801 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2711 4896 5608 5990 Size: 53 83 186 936 1178 2185 712 382 Cuts at: 5990 6625 275 Size: 635 1451 Fragments arranged by size: 2185 1451 1178 936 712 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3566 4113 5070 6234 6632 6906 7053 7464 3566 Size: 547 957 1164 398 274 147 411 3903 Fragments arranged by size: 3903 1164 957 547 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7576 Fragments arranged by size: 7576 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4706 4973 1186 Size: 3520 267 4014 Fragments arranged by size: 4014 3520 267 BseYI C'CCAG_C Cuts at: 2264 2351 2823 2923 3222 3386 3483 3780 4358 Size: 87 472 100 299 164 97 297 578 Cuts at: 4358 4524 4707 4866 5025 5029 7004 7500 2264 Size: 166 183 159 159 4 1975 496 2565 Fragments arranged by size: 2565 1975 578 496 472 299 297 183 166 164 159 159 100 97 87 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2012 2069 3485 3851 3908 4274 4355 Size: 573 276 57 1416 366 57 366 81 Cuts at: 4355 1163 Size: 4609 Fragments arranged by size: 4609 1416 573 366 366 276 81 57 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4930 5097 5251 6012 7040 1540 Size: 275 186 2929 167 154 761 1028 2301 Fragments arranged by size: 2929 2301 1028 761 275 186 167 154 BsiHKAI G_wGCw'C Cuts at: 2115 2751 3621 4194 5862 5947 7018 2115 Size: 636 870 573 1668 85 1071 2898 Fragments arranged by size: 2898 1668 1071 870 636 573 85 BsmI GAATG_Cn' Cuts at: 2908 5312 5405 2908 Size: 2404 93 5304 Fragments arranged by size: 5304 2404 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3986 3986 Size: 7801 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2166 2352 2373 2587 2649 2751 Size: 399 325 51 186 21 214 62 102 Cuts at: 2751 2810 3621 3811 4194 4245 4258 4350 4503 Size: 59 811 190 383 51 13 92 153 Cuts at: 4503 4714 4922 5026 5120 5862 5947 7018 1391 Size: 211 208 104 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 811 742 399 383 325 214 211 208 190 186 153 104 102 94 92 85 62 59 51 51 21 13 BspEI T'CCGG_A Cuts at: 4113 7464 4113 Size: 3351 4450 BspHI T'CATG_A Cuts at: 2409 5690 7420 2409 Size: 3281 1730 2790 Fragments arranged by size: 3281 2790 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 2664 3504 3565 3732 4428 5156 844 Size: 1424 396 840 61 167 696 728 3489 Fragments arranged by size: 3489 1424 840 728 696 396 167 61 BsrBI CCG'CTC Cuts at: 1394 1823 3135 3888 4935 5688 1394 Size: 429 1312 753 1047 753 3507 Fragments arranged by size: 3507 1312 1047 753 753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2200 3832 6296 6470 65 Size: 1183 952 1632 2464 174 1396 Fragments arranged by size: 2464 1632 1396 1183 952 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2302 2495 3566 6445 6632 1700 Size: 110 492 193 1071 2879 187 2869 Fragments arranged by size: 2879 2869 1071 492 193 187 110 BsrGI T'GTAC_A Cuts at: 96 4665 96 Size: 4569 3232 BssHII G'CGCG_C Cuts at: 1925 4846 1925 Size: 2921 4880 BssSI C'ACGA_G Cuts at: 3614 5861 6873 3614 Size: 2247 1012 4542 Fragments arranged by size: 4542 2247 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2509 2805 3505 4876 794 Size: 1715 296 700 1371 3719 Fragments arranged by size: 3719 1715 1371 700 296 BstBI TT'CG_AA Cuts at: 6605 6605 Size: 7801 BstEII G'GTnAC_C Cuts at: 1184 3457 1184 Size: 2273 5528 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2074 3680 5003 2062 Size: 12 1606 1323 4860 Fragments arranged by size: 4860 1606 1323 12 BstYI r'GATC_y Cuts at: 1972 3436 5131 5498 5883 5900 7352 7438 7450 Size: 1464 1695 367 385 17 1452 86 12 Cuts at: 7450 7796 1972 Size: 346 1977 Fragments arranged by size: 1977 1695 1464 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2715 2715 Size: 7801 BtgI C'CryG_G Cuts at: 513 1065 2004 2122 2175 2188 2917 3312 4699 Size: 552 939 118 53 13 729 395 1387 Cuts at: 4699 5091 513 Size: 392 3223 Fragments arranged by size: 3223 1387 939 729 552 395 392 118 53 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7062 Fragments arranged by size: 7062 683 56 BtsI GCAGTG_nn' Cuts at: 3506 4291 4656 5317 6111 6131 3506 Size: 785 365 661 794 20 5176 Fragments arranged by size: 5176 794 785 661 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7766 DraI TTT'AAA Cuts at: 5105 5105 Size: 7801 DraIII CAC_nnn'GTG Cuts at: 2189 2189 Size: 7801 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6808 809 Size: 507 5492 1802 Fragments arranged by size: 5492 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2724 3158 Size: 54 1425 50 275 18 234 660 434 Cuts at: 3158 4927 5094 5248 6137 7766 8 Size: 1769 167 154 889 1629 43 Fragments arranged by size: 1769 1629 1425 889 660 434 275 234 167 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4927 5094 5248 1537 Size: 275 3115 167 154 4090 Fragments arranged by size: 4090 3115 275 167 154 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 5731 1353 Size: 1023 3355 3423 Fragments arranged by size: 3423 3355 1023 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2775 4706 6388 6762 6908 387 Size: 1064 138 1186 1931 1682 374 146 1280 Fragments arranged by size: 1931 1682 1280 1186 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2113 4192 2113 Size: 2079 5722 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2174 2588 2690 2827 3122 3167 Size: 393 467 75 414 102 137 295 45 Cuts at: 3167 3220 3385 3414 3665 3691 3802 4091 4349 Size: 53 165 29 251 26 111 289 258 Cuts at: 4349 4376 4709 4748 4901 5864 6461 7248 7516 Size: 27 333 39 153 963 597 787 268 Cuts at: 7516 1239 Size: 1524 Fragments arranged by size: 1524 963 787 597 467 414 393 333 295 289 268 258 251 165 153 137 111 102 75 53 45 39 29 27 26 EcoNI CCTnn'n_nnAGG Cuts at: 2048 4328 4436 4880 2048 Size: 2280 108 444 4969 Fragments arranged by size: 4969 2280 444 108 EcoO109I rG'GnC_Cy Cuts at: 2719 2998 3240 3358 3649 3808 4075 4242 4396 Size: 279 242 118 291 159 267 167 154 Cuts at: 4396 4417 4441 4528 5087 2719 Size: 21 24 87 559 5433 Fragments arranged by size: 5433 559 291 279 267 242 167 159 154 118 87 24 21 EcoRI G'AATT_C Cuts at: 5110 5488 5110 Size: 378 7423 FspI TGC'GCA Cuts at: 3014 3728 3950 6307 3014 Size: 714 222 2357 4508 Fragments arranged by size: 4508 2357 714 222 HaeII r_GCGC'y Cuts at: 1678 2025 2714 4206 4281 4708 4876 4963 6948 Size: 347 689 1492 75 427 168 87 1985 Cuts at: 6948 1678 Size: 2531 Fragments arranged by size: 2531 1985 1492 689 427 347 168 87 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3178 3210 6442 6474 6516 Size: 32 135 32 1606 32 3232 32 42 Cuts at: 6516 6548 1373 Size: 32 2626 Fragments arranged by size: 3232 2626 1606 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5145 5391 669 Size: 988 281 3207 246 3079 Fragments arranged by size: 3207 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3017 748 Size: 2269 5532 HpaI GTT'AAC Cuts at: 5391 5391 Size: 7801 KasI G'GCGC_C Cuts at: 2710 2710 Size: 7801 KpnI G_GTAC'C Cuts at: 2088 5126 2088 Size: 3038 4763 MfeI C'AATT_G Cuts at: 5400 5400 Size: 7801 MluI A'CGCG_T Cuts at: 7677 7677 Size: 7801 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 3252 6915 7099 1305 Size: 939 1008 3663 184 2007 Fragments arranged by size: 3663 2007 1008 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 2726 10 Size: 54 1425 343 234 660 5085 Fragments arranged by size: 5085 1425 660 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2414 2471 2642 4518 5759 6118 Size: 648 1072 176 57 171 1876 1241 359 Cuts at: 6118 6277 518 Size: 159 2042 Fragments arranged by size: 2042 1876 1241 1072 648 359 176 171 159 57 MspA1I CmG'CkG Cuts at: 1606 2035 2775 3010 3288 3562 3822 4069 4131 Size: 429 740 235 278 274 260 247 62 Cuts at: 4131 4687 5029 5217 5894 7042 7287 1606 Size: 556 342 188 677 1148 245 2120 Fragments arranged by size: 2120 1148 740 677 556 429 342 278 274 260 247 245 235 188 62 NaeI GCC'GGC Cuts at: 2497 2497 Size: 7801 NarI GG'CG_CC Cuts at: 2711 2711 Size: 7801 NcoI C'CATG_G Cuts at: 513 1065 2004 2175 3312 513 Size: 552 939 171 1137 5002 Fragments arranged by size: 5002 1137 939 552 171 NdeI CA'TA_TG Cuts at: 387 3840 387 Size: 3453 4348 NgoMIV G'CCGG_C Cuts at: 2495 2495 Size: 7801 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7801 NotI GC'GGCC_GC Cuts at: 5248 5248 Size: 7801 NsiI A_TGCA'T Cuts at: 2975 2975 Size: 7801 NspI r_CATG'y Cuts at: 1132 1229 2507 3118 3688 5159 6704 1132 Size: 97 1278 611 570 1471 1545 2229 Fragments arranged by size: 2229 1545 1471 1278 611 570 97 PasI CC'CwG_GG Cuts at: 1348 2080 1348 Size: 732 7069 PciI A'CATG_T Cuts at: 6700 6700 Size: 7801 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 1108 Size: 1020 6781 PmeI GTTT'AAAC Cuts at: 5105 5105 Size: 7801 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5870 5902 7383 7415 5870 Size: 32 1481 32 6256 Fragments arranged by size: 6256 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2719 3358 4417 4528 5087 2719 Size: 639 1059 111 559 5433 Fragments arranged by size: 5433 1059 639 559 111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7801 PsiI TTA'TAA Cuts at: 5371 5371 Size: 7801 PspOMI G'GGCC_C Cuts at: 3807 4241 5022 3807 Size: 434 781 6586 Fragments arranged by size: 6586 781 434 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2948 2980 2948 Size: 32 7769 PstI C_TGCA'G Cuts at: 830 2558 3201 3351 3416 3501 3576 3794 5153 Size: 1728 643 150 65 85 75 218 1359 Cuts at: 5153 830 Size: 3478 Fragments arranged by size: 3478 1728 1359 643 218 150 85 75 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7801 PvuII CAG'CTG Cuts at: 3010 4069 4131 4687 5029 3010 Size: 1059 62 556 342 5782 Fragments arranged by size: 5782 1059 556 342 62 RsrII CG'GwC_CG Cuts at: 4825 4825 Size: 7801 SacI G_AGCT'C Cuts at: 2115 4194 2115 Size: 2079 5722 SalI G'TCGA_C Cuts at: 1936 5143 1936 Size: 3207 4594 SanDI GG'GwC_CC Cuts at: 5087 5087 Size: 7801 SapI GCTCTTCn'nnn_ Cuts at: 2376 2376 Size: 7801 SbfI CC_TGCA'GG Cuts at: 2558 5153 2558 Size: 2595 5206 ScaI AGT'ACT Cuts at: 6049 7761 6049 Size: 1712 6089 SexAI A'CCwGG_T Cuts at: 3453 3453 Size: 7801 SfcI C'TryA_G Cuts at: 826 1045 2554 3197 3347 3412 3497 3572 3790 Size: 219 1509 643 150 65 85 75 218 Cuts at: 3790 3968 5149 6284 6965 7156 826 Size: 178 1181 1135 681 191 1471 Fragments arranged by size: 1509 1471 1181 1135 681 643 219 218 191 178 150 85 75 65 SfoI GGC'GCC Cuts at: 2712 2712 Size: 7801 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7801 SmaI CCC'GGG Cuts at: 1872 2580 5128 1872 Size: 708 2548 4545 Fragments arranged by size: 4545 2548 708 SmlI C'TyrA_G Cuts at: 820 1017 1943 2285 2621 4214 5905 6806 7068 Size: 197 926 342 336 1593 1691 901 262 Cuts at: 7068 820 Size: 1553 Fragments arranged by size: 1691 1593 1553 926 901 342 336 262 197 SnaBI TAC'GTA Cuts at: 493 5592 493 Size: 5099 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7801 SphI G_CATG'C Cuts at: 1132 2507 3688 5159 1132 Size: 1375 1181 1471 3774 Fragments arranged by size: 3774 1471 1375 1181 SspI AAT'ATT Cuts at: 5 52 5725 5 Size: 47 5673 2081 Fragments arranged by size: 5673 2081 47 StuI AGG'CCT Cuts at: 1517 2868 2990 3882 1517 Size: 1351 122 892 5436 Fragments arranged by size: 5436 1351 892 122 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 2590 2985 3312 3432 3877 Size: 552 939 171 415 395 327 120 445 Cuts at: 3877 4170 4176 5240 513 Size: 293 6 1064 3074 Fragments arranged by size: 3074 1064 939 552 445 415 395 327 293 171 120 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2624 4484 4651 4771 5828 5845 5998 Size: 1255 1044 1860 167 120 1057 17 153 Cuts at: 5998 6183 6628 325 Size: 185 445 1498 Fragments arranged by size: 1860 1498 1255 1057 1044 445 185 167 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 4665 6047 Size: 274 80 33 51 1443 648 2040 1382 Cuts at: 6047 7759 96 Size: 1712 138 Fragments arranged by size: 2040 1712 1443 1382 648 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7761 7793 7761 Size: 32 7769 Tth111I GACn'n_nGTC Cuts at: 1316 4829 1316 Size: 3513 4288 XbaI T'CTAG_A Cuts at: 5137 5137 Size: 7801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2797 4235 4858 5000 2359 Size: 438 1438 623 142 5160 Fragments arranged by size: 5160 1438 623 438 142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7801 XmaI C'CCGG_G Cuts at: 1870 2578 5126 1870 Size: 708 2548 4545 Fragments arranged by size: 4545 2548 708 XmnI GAAnn'nnTTC Cuts at: 5930 5930 Size: 7801 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4897 5609 276 Size: 53 83 186 936 3363 712 2468 Fragments arranged by size: 3363 2468 936 712 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspXI SacII SfiI SrfI SwaI