(Linear) (Six-base) MAPSORT of: KIEE3348.seq Check: 7563 from: 1 to: 3096 KIEE3348.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 263 1499 1560 1727 3096 Size: 263 1236 61 167 1369 Fragments arranged by size: 1369 1236 263 167 61 AatII G_ACGT'C Cuts at: 0 2894 3096 Size: 2894 202 Acc65I G'GTAC_C Cuts at: 0 79 3096 Size: 79 3017 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 822 1117 1215 1380 1409 1686 1797 Size: 169 653 295 98 165 29 277 111 Cuts at: 1797 2086 3096 Size: 289 1010 Fragments arranged by size: 1010 653 295 289 277 169 165 111 98 29 AgeI A'CCGG_T Cuts at: 0 1561 3096 Size: 1561 1535 AhdI GACnn_n'nnGTC Cuts at: 0 1187 3096 Size: 1187 1909 AleI CACnn'nnGTG Cuts at: 0 637 2513 3096 Size: 637 1876 583 Fragments arranged by size: 1876 637 583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 37 3096 Size: 37 3059 AlwNI CAG_nnn'CTG Cuts at: 0 561 1185 1500 1551 1971 2415 2871 3014 Size: 561 624 315 51 420 444 456 143 Cuts at: 3014 3096 Size: 82 Fragments arranged by size: 624 561 456 444 420 315 143 82 51 ApaI G_GGCC'C Cuts at: 0 1806 2240 3021 3096 Size: 1806 434 781 75 Fragments arranged by size: 1806 781 434 75 AvaI C'yCGr_G Cuts at: 0 573 3096 Size: 573 2523 BanI G'GyrC_C Cuts at: 0 79 577 639 705 2098 3096 Size: 79 498 62 66 1393 998 Fragments arranged by size: 1393 998 498 79 66 62 BanII G_rGCy'C Cuts at: 0 110 347 1806 2189 2240 2917 3021 3096 Size: 110 237 1459 383 51 677 104 75 Fragments arranged by size: 1459 677 383 237 110 104 75 51 BbeI G_GCGC'C Cuts at: 0 709 3096 Size: 709 2387 BbsI GAAGACnn'nnnn_ Cuts at: 0 1397 2616 3096 Size: 1397 1219 480 Fragments arranged by size: 1397 1219 480 BbvCI CC'TCA_GC Cuts at: 0 307 448 1654 1738 2395 3096 Size: 307 141 1206 84 657 701 Fragments arranged by size: 1206 701 657 307 141 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2124 2158 2235 2269 2874 2908 3096 Size: 2124 34 77 34 605 34 188 Fragments arranged by size: 2124 605 188 77 34 34 34 BfrBI ATG'CAT Cuts at: 0 968 3096 Size: 968 2128 BglI GCCn_nnn'nGGC Cuts at: 0 491 2299 2921 3096 Size: 491 1808 622 175 Fragments arranged by size: 1808 622 491 175 Bme1580I G_kGCm'C Cuts at: 0 161 368 582 644 805 1806 2240 2253 Size: 161 207 214 62 161 1001 434 13 Cuts at: 2253 2345 2498 2709 3021 3096 Size: 92 153 211 312 75 Fragments arranged by size: 1001 434 312 214 211 207 161 161 153 92 75 62 13 BmrI ACTGGGnnnn_n' Cuts at: 0 1363 1539 1837 2664 2990 3096 Size: 1363 176 298 827 326 106 Fragments arranged by size: 1363 827 326 298 176 106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2457 3096 Size: 2457 639 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 583 685 1162 1660 2344 2371 2704 Size: 94 489 102 477 498 684 27 333 Cuts at: 2704 2743 2896 3096 Size: 39 153 200 Fragments arranged by size: 684 498 489 477 333 200 153 102 94 39 27 Bpu10I CC'TnA_GC Cuts at: 0 307 388 448 847 1507 1654 1738 1879 Size: 307 81 60 399 660 147 84 141 Cuts at: 1879 2194 2395 2911 3096 Size: 315 201 516 185 Fragments arranged by size: 660 516 399 315 307 201 185 147 141 84 81 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 601 2194 3096 Size: 265 336 1593 902 Fragments arranged by size: 1593 902 336 265 BsaI GGTCTCn'nnnn_ Cuts at: 0 1135 1844 2839 3096 Size: 1135 709 995 257 Fragments arranged by size: 1135 995 709 257 BsaAI yAC'GTr Cuts at: 0 1257 2940 3096 Size: 1257 1683 156 Fragments arranged by size: 1683 1257 156 BsaHI Gr'CG_yC Cuts at: 0 706 2891 3096 Size: 706 2185 205 Fragments arranged by size: 2185 706 205 BsaWI w'CCGG_w Cuts at: 0 1561 2108 3065 3096 Size: 1561 547 957 31 Fragments arranged by size: 1561 957 547 31 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2701 2968 3096 Size: 2701 267 128 Fragments arranged by size: 2701 267 128 BseYI C'CCAG_C Cuts at: 0 259 346 818 918 1217 1381 1478 1775 Size: 259 87 472 100 299 164 97 297 Cuts at: 1775 2353 2519 2702 2861 3020 3024 3096 Size: 578 166 183 159 159 4 72 Fragments arranged by size: 578 472 299 297 259 183 166 164 159 159 100 97 87 72 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 7 64 1480 1846 1903 2269 2350 3096 Size: 7 57 1416 366 57 366 81 746 Fragments arranged by size: 1416 746 366 366 81 57 57 7 BsiEI CG_ry'CG Cuts at: 0 2925 3092 3096 Size: 2925 167 4 Fragments arranged by size: 2925 167 4 BsiHKAI G_wGCw'C Cuts at: 0 110 746 1616 2189 3096 Size: 110 636 870 573 907 Fragments arranged by size: 907 870 636 573 110 BsmI GAATG_Cn' Cuts at: 0 903 3096 Size: 903 2193 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1981 3096 Size: 1981 1115 Bsp1286I G_dGCh'C Cuts at: 0 110 161 347 368 582 644 746 805 Size: 110 51 186 21 214 62 102 59 Cuts at: 805 1616 1806 2189 2240 2253 2345 2498 2709 Size: 811 190 383 51 13 92 153 211 Cuts at: 2709 2917 3021 3096 Size: 208 104 75 Fragments arranged by size: 811 383 214 211 208 190 186 153 110 104 102 92 75 62 59 51 51 21 13 BspEI T'CCGG_A Cuts at: 0 2108 3096 Size: 2108 988 BspHI T'CATG_A Cuts at: 0 404 3096 Size: 404 2692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 263 659 1499 1560 1727 2423 3096 Size: 263 396 840 61 167 696 673 Fragments arranged by size: 840 696 673 396 263 167 61 BsrBI CCG'CTC Cuts at: 0 1130 1883 2930 3096 Size: 1130 753 1047 166 Fragments arranged by size: 1130 1047 753 166 BsrDI GCAATG_nn' Cuts at: 0 195 1827 3096 Size: 195 1632 1269 Fragments arranged by size: 1632 1269 195 BsrFI r'CCGG_y Cuts at: 0 297 490 1561 3096 Size: 297 193 1071 1535 Fragments arranged by size: 1535 1071 297 193 BsrGI T'GTAC_A Cuts at: 0 2660 3096 Size: 2660 436 BssHII G'CGCG_C Cuts at: 0 2841 3096 Size: 2841 255 BssSI C'ACGA_G Cuts at: 0 1609 3096 Size: 1609 1487 BstAPI GCAn_nnn'nTGC Cuts at: 0 504 800 1500 2871 3096 Size: 504 296 700 1371 225 Fragments arranged by size: 1371 700 504 296 225 BstEII G'GTnAC_C Cuts at: 0 1452 3096 Size: 1452 1644 BstXI CCAn_nnnn'nTGG Cuts at: 0 57 69 1675 2998 3096 Size: 57 12 1606 1323 98 Fragments arranged by size: 1606 1323 98 57 12 BstYI r'GATC_y Cuts at: 0 1431 3096 Size: 1431 1665 Bsu36I CC'TnA_GG Cuts at: 0 710 3096 Size: 710 2386 BtgI C'CryG_G Cuts at: 0 117 170 183 912 1307 2694 3086 3096 Size: 117 53 13 729 395 1387 392 10 Fragments arranged by size: 1387 729 395 392 117 53 13 10 BtsI GCAGTG_nn' Cuts at: 0 1501 2286 2651 3096 Size: 1501 785 365 445 Fragments arranged by size: 1501 785 445 365 DraIII CAC_nnn'GTG Cuts at: 0 184 3096 Size: 184 2912 EaeI y'GGCC_r Cuts at: 0 59 719 1153 2922 3089 3096 Size: 59 660 434 1769 167 7 Fragments arranged by size: 1769 660 434 167 59 7 EagI C'GGCC_G Cuts at: 0 2922 3089 3096 Size: 2922 167 7 Fragments arranged by size: 2922 167 7 EarI CTCTTCn'nnn_ Cuts at: 0 371 3096 Size: 371 2725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 770 2701 3096 Size: 770 1931 395 Fragments arranged by size: 1931 770 395 EcoICRI GAG'CTC Cuts at: 0 108 2187 3096 Size: 108 2079 909 Fragments arranged by size: 2079 909 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 169 583 685 822 1117 1162 1215 Size: 94 75 414 102 137 295 45 53 Cuts at: 1215 1380 1409 1660 1686 1797 2086 2344 2371 Size: 165 29 251 26 111 289 258 27 Cuts at: 2371 2704 2743 2896 3096 Size: 333 39 153 200 Fragments arranged by size: 414 333 295 289 258 251 200 165 153 137 111 102 94 75 53 45 39 29 27 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 43 2323 2431 2875 3096 Size: 43 2280 108 444 221 Fragments arranged by size: 2280 444 221 108 43 EcoO109I rG'GnC_Cy Cuts at: 0 714 993 1235 1353 1644 1803 2070 2237 Size: 714 279 242 118 291 159 267 167 Cuts at: 2237 2391 2412 2436 2523 3082 3096 Size: 154 21 24 87 559 14 Fragments arranged by size: 714 559 291 279 267 242 167 159 154 118 87 24 21 14 FspI TGC'GCA Cuts at: 0 1009 1723 1945 3096 Size: 1009 714 222 1151 Fragments arranged by size: 1151 1009 714 222 HaeII r_GCGC'y Cuts at: 0 20 709 2201 2276 2703 2871 2958 3096 Size: 20 689 1492 75 427 168 87 138 Fragments arranged by size: 1492 689 427 168 138 87 75 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1173 1205 3096 Size: 1173 32 1891 Fragments arranged by size: 1891 1173 32 HindIII A'AGCT_T Cuts at: 0 1012 3096 Size: 1012 2084 KasI G'GCGC_C Cuts at: 0 705 3096 Size: 705 2391 KpnI G_GTAC'C Cuts at: 0 83 3096 Size: 83 3013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 239 1247 3096 Size: 239 1008 1849 Fragments arranged by size: 1849 1008 239 MscI TGG'CCA Cuts at: 0 61 721 3096 Size: 61 660 2375 Fragments arranged by size: 2375 660 61 MslI CAynn'nnrTG Cuts at: 0 233 409 466 637 2513 3096 Size: 233 176 57 171 1876 583 Fragments arranged by size: 1876 583 233 176 171 57 MspA1I CmG'CkG Cuts at: 0 30 770 1005 1283 1557 1817 2064 2126 Size: 30 740 235 278 274 260 247 62 Cuts at: 2126 2682 3024 3096 Size: 556 342 72 Fragments arranged by size: 740 556 342 278 274 260 247 235 72 62 30 NaeI GCC'GGC Cuts at: 0 492 3096 Size: 492 2604 NarI GG'CG_CC Cuts at: 0 706 3096 Size: 706 2390 NcoI C'CATG_G Cuts at: 0 170 1307 3096 Size: 170 1137 1789 Fragments arranged by size: 1789 1137 170 NdeI CA'TA_TG Cuts at: 0 1835 3096 Size: 1835 1261 NgoMIV G'CCGG_C Cuts at: 0 490 3096 Size: 490 2606 NsiI A_TGCA'T Cuts at: 0 970 3096 Size: 970 2126 NspI r_CATG'y Cuts at: 0 502 1113 1683 3096 Size: 502 611 570 1413 Fragments arranged by size: 1413 611 570 502 PasI CC'CwG_GG Cuts at: 0 75 3096 Size: 75 3021 PflMI CCAn_nnn'nTGG Cuts at: 0 123 3096 Size: 123 2973 PpuMI rG'GwC_Cy Cuts at: 0 714 1353 2412 2523 3082 3096 Size: 714 639 1059 111 559 14 Fragments arranged by size: 1059 714 639 559 111 14 PspOMI G'GGCC_C Cuts at: 0 1802 2236 3017 3096 Size: 1802 434 781 79 Fragments arranged by size: 1802 781 434 79 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 943 975 3096 Size: 943 32 2121 Fragments arranged by size: 2121 943 32 PstI C_TGCA'G Cuts at: 0 553 1196 1346 1411 1496 1571 1789 3096 Size: 553 643 150 65 85 75 218 1307 Fragments arranged by size: 1307 643 553 218 150 85 75 65 PvuII CAG'CTG Cuts at: 0 1005 2064 2126 2682 3024 3096 Size: 1005 1059 62 556 342 72 Fragments arranged by size: 1059 1005 556 342 72 62 RsrII CG'GwC_CG Cuts at: 0 2820 3096 Size: 2820 276 SacI G_AGCT'C Cuts at: 0 110 2189 3096 Size: 110 2079 907 Fragments arranged by size: 2079 907 110 SanDI GG'GwC_CC Cuts at: 0 3082 3096 Size: 3082 14 SapI GCTCTTCn'nnn_ Cuts at: 0 371 3096 Size: 371 2725 SbfI CC_TGCA'GG Cuts at: 0 553 3096 Size: 553 2543 SexAI A'CCwGG_T Cuts at: 0 1448 3096 Size: 1448 1648 SfcI C'TryA_G Cuts at: 0 549 1192 1342 1407 1492 1567 1785 1963 Size: 549 643 150 65 85 75 218 178 Cuts at: 1963 3096 Size: 1133 Fragments arranged by size: 1133 643 549 218 178 150 85 75 65 SfoI GGC'GCC Cuts at: 0 707 3096 Size: 707 2389 SmaI CCC'GGG Cuts at: 0 575 3096 Size: 575 2521 SmlI C'TyrA_G Cuts at: 0 280 616 2209 3096 Size: 280 336 1593 887 Fragments arranged by size: 1593 887 336 280 SphI G_CATG'C Cuts at: 0 502 1683 3096 Size: 502 1181 1413 Fragments arranged by size: 1413 1181 502 StuI AGG'CCT Cuts at: 0 863 985 1877 3096 Size: 863 122 892 1219 Fragments arranged by size: 1219 892 863 122 StyI C'CwwG_G Cuts at: 0 170 585 980 1307 1427 1872 2165 2171 Size: 170 415 395 327 120 445 293 6 Cuts at: 2171 3096 Size: 925 Fragments arranged by size: 925 445 415 395 327 293 170 120 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 619 2479 2646 2766 3096 Size: 619 1860 167 120 330 Fragments arranged by size: 1860 619 330 167 120 TatI w'GTAC_w Cuts at: 0 620 2660 3096 Size: 620 2040 436 Fragments arranged by size: 2040 620 436 Tth111I GACn'n_nGTC Cuts at: 0 2824 3096 Size: 2824 272 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 354 792 2230 2853 2995 3096 Size: 354 438 1438 623 142 101 Fragments arranged by size: 1438 623 438 354 142 101 XmaI C'CCGG_G Cuts at: 0 573 3096 Size: 573 2523 ZraI GAC'GTC Cuts at: 0 2892 3096 Size: 2892 204 Enzymes that do cut: AarI AatII Acc65I AcuI AgeI AhdI AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PflMI PpuMI PspOMI PsrI PstI PvuII RsrII SacI SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AflIII AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BglII BlpI BmgBI BmtI BsaBI BsaXI BsiWI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI HincII HpaI MfeI MluI NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI SacII SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI XbaI XhoI XmnI