Restriction map for
pFN21AE2792
(Linear) (Six-base) MAPSORT of: KIEE2792.seq Check: 6647 from: 1 to: 630
KIEE2792.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 630
Size: 535 95
AlwNI CAG_nnn'CTG
Cuts at: 0 150 241 630
Size: 150 91 389
Fragments arranged by size:
389 150 91
ApaI G_GGCC'C
Cuts at: 0 310 630
Size: 310 320
AvaI C'yCGr_G
Cuts at: 0 384 630
Size: 384 246
BanI G'GyrC_C
Cuts at: 0 28 560 630
Size: 28 532 70
Fragments arranged by size:
532 70 28
BanII G_rGCy'C
Cuts at: 0 310 626 630
Size: 310 316 4
Fragments arranged by size:
316 310 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 630
Size: 457 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 137 630
Size: 103 34 493
Fragments arranged by size:
493 103 34
BclI T'GATC_A
Cuts at: 0 320 542 630
Size: 320 222 88
Fragments arranged by size:
320 222 88
Bme1580I G_kGCm'C
Cuts at: 0 31 221 310 563 630
Size: 31 190 89 253 67
Fragments arranged by size:
253 190 89 67 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 610 630
Size: 440 170 20
Fragments arranged by size:
440 170 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 470 630
Size: 130 340 160
Fragments arranged by size:
340 160 130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 601 630
Size: 120 481 29
Fragments arranged by size:
481 120 29
BseYI C'CCAG_C
Cuts at: 0 303 377 630
Size: 303 74 253
Fragments arranged by size:
303 253 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 145 396 630
Size: 61 84 251 234
Fragments arranged by size:
251 234 84 61
BsiHKAI G_wGCw'C
Cuts at: 0 512 630
Size: 512 118
Bsp1286I G_dGCh'C
Cuts at: 0 31 221 310 512 563 626 630
Size: 31 190 89 202 51 63 4
Fragments arranged by size:
202 190 89 63 51 31 4
BspHI T'CATG_A
Cuts at: 0 545 630
Size: 545 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 630
Size: 212 418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 241 630
Size: 241 389
BstXI CCAn_nnnn'nTGG
Cuts at: 0 590 630
Size: 590 40
BtgI C'CryG_G
Cuts at: 0 488 630
Size: 488 142
BtsI GCAGTG_nn'
Cuts at: 0 87 157 630
Size: 87 70 473
Fragments arranged by size:
473 87 70
DraIII CAC_nnn'GTG
Cuts at: 0 337 537 630
Size: 337 200 93
Fragments arranged by size:
337 200 93
EaeI y'GGCC_r
Cuts at: 0 194 630
Size: 194 436
EarI CTCTTCn'nnn_
Cuts at: 0 61 630
Size: 61 569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 535 610 630
Size: 440 95 75 20
Fragments arranged by size:
440 95 75 20
EcoO109I rG'GnC_Cy
Cuts at: 0 51 69 307 483 630
Size: 51 18 238 176 147
Fragments arranged by size:
238 176 147 51 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 444 476 630
Size: 444 32 154
Fragments arranged by size:
444 154 32
MscI TGG'CCA
Cuts at: 0 196 630
Size: 196 434
MslI CAynn'nnrTG
Cuts at: 0 37 630
Size: 37 593
MspA1I CmG'CkG
Cuts at: 0 522 630
Size: 522 108
NcoI C'CATG_G
Cuts at: 0 488 630
Size: 488 142
NspI r_CATG'y
Cuts at: 0 345 630
Size: 345 285
PpuMI rG'GwC_Cy
Cuts at: 0 483 630
Size: 483 147
PspOMI G'GGCC_C
Cuts at: 0 306 630
Size: 306 324
PstI C_TGCA'G
Cuts at: 0 620 630
Size: 620 10
PvuII CAG'CTG
Cuts at: 0 522 630
Size: 522 108
SbfI CC_TGCA'GG
Cuts at: 0 620 630
Size: 620 10
SfcI C'TryA_G
Cuts at: 0 616 630
Size: 616 14
SmaI CCC'GGG
Cuts at: 0 386 630
Size: 386 244
StyI C'CwwG_G
Cuts at: 0 224 296 488 630
Size: 224 72 192 142
Fragments arranged by size:
224 192 142 72
TatI w'GTAC_w
Cuts at: 0 102 344 630
Size: 102 242 286
Fragments arranged by size:
286 242 102
XmaI C'CCGG_G
Cuts at: 0 384 630
Size: 384 246
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbsI BcgI
BclI Bme1580I BpmI BsaI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BstAPI BstXI BtgI BtsI DraIII
EaeI EarI Eco57MI EcoO109I Hin4I MscI MslI MspA1I
NcoI NspI PpuMI PspOMI PstI PvuII SbfI SfcI
SmaI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI