################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BssHII BssSI BstBI BstEII BstPI BstUI CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp509I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 730 GTATAC GT^[AC][GT]AC Alw44I 432 GTGCAC G^TGCAC AlwI 939 GATCC GGATC(4/5) AocI 422 CCTCAGG CC^T[ACGT]AGG ApaLI 432 GTGCAC G^TGCAC AspI 112 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 659 TGCGCA TGC^GCA BanI 384 GGTGCC G^G[CT][GA]CC BbsI 85 GAAGAC GAAGAC(2/6) BciVI 984 GTATCC GTATCC(6/5) BglII 1058 AGATCT A^GATCT BpuAI 85 GAAGAC GAAGAC(2/6) BsaAI 799 TACGTG [CT]AC^GT[GA] BsaI 884 GAGACC GGTCTC(1/5) BsaWI 498 TCCGGT [AT]^CCGG[AT] BsmI 22 GAATGC GAATGC(1/-1) Bsp1407I 125 TGTACA T^GTACA BspHI 392 TCATGA T^CATGA BsrBI 1099 GAGCGG CCGCTC(-3/-3) BsrFI 428 GCCGGT [GA]^CCGG[CT] BsrGI 125 TGTACA T^GTACA Bst1107I 730 GTATAC GTA^TAC BstXI 398 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1058 AGATCT [GA]^GATC[CT] BstZ17I 730 GTATAC GTA^TAC Bsu36I 422 CCTCAGG CC^T[ACGT]AGG Cfr10I 428 GCCGGT [GA]^CCGG[CT] DrdI 699 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 359 CCATGG C^C[GA][CT]GG Eco81I 422 CCTCAGG CC^T[ACGT]AGG EcoNI 777 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 24 ATGCAT ATGCA^T FauI 993 GCGGG CCCGC(4/6) FspI 659 TGCGCA TGC^GCA HindIII 157 AAGCTT A^AGCTT MaeII 800 ACGT A^CGT MflI 1058 AGATCT [GA]^GATC[CT] MseI 305 TTAA T^TAA NcoI 359 CCATGG C^CATGG NsiI 24 ATGCAT ATGCA^T PaeR7I 292 CTCGAG C^TCGAG PflFI 112 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 910 CAGCTG CAG^CTG RcaI 392 TCATGA T^CATGA SacI 646 GAGCTC GAGCT^C SapI 1006 GAAGAGC GCTCTTC(1/4) ScaI 719 AGTACT AGT^ACT SmlI 292 CTCGAG C^T[CT][GA]AG SspBI 125 TGTACA T^GTACA Tru9I 305 TTAA T^TAA Tth111I 112 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 292 CTCGAG C^TCGAG XhoII 1058 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AspHI 432 GTGCAC G[AT]GC[AT]^C 646 GAGCTC BcnI 834 CCGGG CC^[GC]GG 1025 CCCGG BfaI 334 CTAG C^TAG 340 CTAG BpmI 715 CTCCAG CTGGAG(16/14) 406 CTGGAG BsiHKAI 432 GTGCAC G[AT]GC[AT]^C 646 GAGCTC BsmAI 884 GAGAC GTCTC(1/5) 830 GTCTC BspMI 283 ACCTGC ACCTGC(4/8) 677 ACCTGC BsrDI 663 CATTGC GCAATG(2/0) 233 GCAATG BstAPI 5 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 17 GCATCGAATGC CfoI 287 GCGC GCG^C 660 GCGC DdeI 423 CTCAG C^T[ACGT]AG 862 CTGAG EaeI 487 CGGCCA [CT]^GGCC[GA] 894 TGGCCG EarI 244 CTCTTC CTCTTC(1/4) 1006 GAAGAG EcoT14I 359 CCATGG C^C[AT][AT]GG 746 CCAAGG HhaI 287 GCGC GCG^C 660 GCGC HinP1I 287 GCGC G^CGC 660 GCGC HinfI 451 GAGTC G^A[ACGT]TC 999 GACTC Ksp632I 244 CTCTTC CTCTTC(1/4) 1006 GAAGAG MaeI 334 CTAG C^TAG 340 CTAG NciI 834 CCGGG CC^[GC]GG 1025 CCCGG NspI 787 GCATGT [GA]CATG^[CT] 918 GCATGT PflMI 753 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1086 CCATGAGCTGG PleI 999 GACTC GAGTC(4/5) 451 GAGTC SexAI 653 ACCTGGT A^CC[AT]GGT 758 ACCTGGT StyI 359 CCATGG C^C[AT][AT]GG 746 CCAAGG Van91I 753 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1086 CCATGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I