Restriction map for
pFN21AE3160
(Circular) (Six-base) MAPSORT of: pFN21AE3160.seq Check: 8642 from: 1 to: 5845
pFN21AE3160 5845 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2736 3188 1937
Size: 799 452 4594
Fragments arranged by size:
4594 799 452
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2109 3908 5292 5560 1632
Size: 391 86 1799 1384 268 1917
Fragments arranged by size:
1917 1799 1384 391 268 86
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 4129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2900 1184
Size: 1716 4129
AlwNI CAG_nnn'CTG
Cuts at: 2016 2683 2764 2917 3588 5160 2016
Size: 667 81 153 671 1572 2701
Fragments arranged by size:
2701 1572 671 667 153 81
ApaLI G'TGCA_C
Cuts at: 2437 3902 5058 2437
Size: 1465 1156 3224
Fragments arranged by size:
3224 1465 1156
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 2297 3006 3170 1870
Size: 73 160 194 709 164 4545
Fragments arranged by size:
4545 709 194 164 160 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2972 3005 2972
Size: 33 5812
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 3166 4621 618
Size: 325 217 627 602 777 1455 1842
Fragments arranged by size:
1842 1455 777 627 602 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 2655 2873 3164 1391
Size: 913 351 218 291 4072
Fragments arranged by size:
4072 913 351 291 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 928
Size: 971 198 4676
Fragments arranged by size:
4676 971 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3000 3737 4947 3000
Size: 737 1210 3898
Fragments arranged by size:
3898 1210 737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BfrBI ATG'CAT
Cuts at: 2031 2031
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3063 5840 3063
Size: 2777 3068
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2394 2441 2823 3906 5062 1790
Size: 604 47 382 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 604 382 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 4534 442
Size: 416 53 83 399 24 2159 958 1753
Fragments arranged by size:
2159 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2705 4505 1239
Size: 1193 273 1800 2579
Fragments arranged by size:
2579 1800 1193 273
Bpu10I CC'TnA_GC
Cuts at: 2867 2867
Size: 5845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2883 4508 882
Size: 617 1384 1625 2219
Fragments arranged by size:
2219 1625 1384 617
BsaAI yAC'GTr
Cuts at: 493 1204 2806 3636 493
Size: 711 1602 830 2702
Fragments arranged by size:
2702 1602 830 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3652 4034 4669 275
Size: 53 83 186 936 2119 382 635 1451
Fragments arranged by size:
2119 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2503 4278 4676 4950 5097 5508 2503
Size: 1775 398 274 147 411 2840
Fragments arranged by size:
2840 1775 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 4909
Fragments arranged by size:
4909 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2384 2429 1186
Size: 1051 147 45 4602
Fragments arranged by size:
4602 1051 147 45
BseYI C'CCAG_C
Cuts at: 2059 2910 5048 5544 2059
Size: 851 2138 496 2360
Fragments arranged by size:
2360 2138 851 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2441 2655 3906 3991 5062 2441
Size: 214 1251 85 1071 3224
Fragments arranged by size:
3224 1251 1071 214 85
BsmI GAATG_Cn'
Cuts at: 2033 3356 3449 2033
Size: 1323 93 4429
Fragments arranged by size:
4429 1323 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2394 2441 2655 2823 2873 3164
Size: 399 514 90 47 214 168 50 291
Cuts at: 3164 3906 3991 5062 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 399 291 214 168
90 85 50 47
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 2397 3734 5464 2397
Size: 1337 1730 2778
Fragments arranged by size:
2778 1730 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2297 2691 3200 844
Size: 1453 394 509 3489
Fragments arranged by size:
3489 1453 509 394
BsrBI CCG'CTC
Cuts at: 1394 1823 3106 3732 1394
Size: 429 1283 626 3507
Fragments arranged by size:
3507 1283 626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2245 2667 4340 4514 65
Size: 1183 997 422 1673 174 1396
Fragments arranged by size:
1673 1396 1183 997 422 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2433 4489 4676 1700
Size: 110 623 2056 187 2869
Fragments arranged by size:
2869 2056 623 187 110
BsrGI T'GTAC_A
Cuts at: 96 2130 96
Size: 2034 3811
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2016 2028 794
Size: 1222 12 4611
Fragments arranged by size:
4611 1222 12
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2410
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 3063 3175 3542 3927 3944 5396 5482 5494
Size: 1091 112 367 385 17 1452 86 12
Cuts at: 5494 5840 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1091 385 367 346 112 86
17 12
BstZ17I GTA'TAC
Cuts at: 2737 2737
Size: 5845
Bsu36I CC'TnA_GG
Cuts at: 2428 2428
Size: 5845
BtgI C'CryG_G
Cuts at: 513 1065 2004 2364 513
Size: 552 939 360 3994
Fragments arranged by size:
3994 939 552 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 3361 4155 4175 3361
Size: 794 20 5031
Fragments arranged by size:
5031 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2710 4852 809
Size: 507 1394 2142 1802
Fragments arranged by size:
2142 1802 1394 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2492 2899 3292
Size: 54 1425 50 275 18 662 407 393
Cuts at: 3292 4181 5810 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 662 407 393 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2255 3006 3775 1353
Size: 902 751 769 3423
Fragments arranged by size:
3423 902 769 751
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2603 4432 4806 4952 387
Size: 1064 138 1014 1829 374 146 1280
Fragments arranged by size:
1829 1280 1064 1014 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 2653
Size: 5845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2109 2432 2705 3908 4505 5292
Size: 393 391 86 323 273 1203 597 787
Cuts at: 5292 5560 1239
Size: 268 1524
Fragments arranged by size:
1524 1203 787 597 393 391 323 273
268 86
EcoNI CCTnn'n_nnAGG
Cuts at: 2786 2786
Size: 5845
EcoO109I rG'GnC_Cy
Cuts at: 2063 2083 2572 2761 2063
Size: 20 489 189 5147
Fragments arranged by size:
5147 489 189 20
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FspI TGC'GCA
Cuts at: 2666 4351 2666
Size: 1685 4160
FspAI rTGC'GCAy
Cuts at: 2666 2666
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2055 2062 2084 2087 2094
Size: 32 135 32 483 7 22 3 7
Cuts at: 2094 2116 2188 2220 2762 2794 2818 2850 4486
Size: 22 72 32 542 32 24 32 1636
Cuts at: 4486 4518 4560 4592 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1636 542 483 135 72 42 32
32 32 32 32 32 32 24 22
22 7 7 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 4431
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2484 4959 5143 1305
Size: 1179 2475 184 2007
Fragments arranged by size:
2475 2007 1179 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2882 2928 3054 3803 4162 4321
Size: 648 1014 702 46 126 749 359 159
Cuts at: 4321 518
Size: 2042
Fragments arranged by size:
2042 1014 749 702 648 359 159 126
46
MspA1I CmG'CkG
Cuts at: 1606 2013 2491 2917 3261 3938 5086 5331 1606
Size: 407 478 426 344 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 478 426 407 344 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2364 513
Size: 552 939 360 3994
Fragments arranged by size:
3994 939 552 360
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NsiI A_TGCA'T
Cuts at: 2033 2033
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2796 2927 3203 4748 1132
Size: 97 1567 131 276 1545 2229
Fragments arranged by size:
2229 1567 1545 276 131 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 3097 1108
Size: 1656 333 3856
Fragments arranged by size:
3856 1656 333
PfoI T'CCnGG_A
Cuts at: 2757 2838 2757
Size: 81 5764
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2063 2083 2572 2761 2063
Size: 20 489 189 5147
Fragments arranged by size:
5147 489 189 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspXI vC'TCGA_Gb
Cuts at: 2297 2297
Size: 5845
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 5813
PstI C_TGCA'G
Cuts at: 830 2157 2864 2922 3197 830
Size: 1327 707 58 275 3478
Fragments arranged by size:
3478 1327 707 275 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2917 2917
Size: 5845
SacI G_AGCT'C
Cuts at: 2655 2655
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SanDI GG'GwC_CC
Cuts at: 2063 2063
Size: 5845
SapI GCTCTTCn'nnn_
Cuts at: 3006 3006
Size: 5845
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 2726 4093 5805 2726
Size: 1367 1712 2766
Fragments arranged by size:
2766 1712 1367
SexAI A'CCwGG_T
Cuts at: 2658 2763 2658
Size: 105 5740
SfcI C'TryA_G
Cuts at: 826 1045 2153 2860 2918 2981 3193 4328 5009
Size: 219 1108 707 58 63 212 1135 681
Cuts at: 5009 5200 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1108 707 681 219 212 191
63 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 3949 4850 5112 820
Size: 197 926 354 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 354 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 2751 3284 513
Size: 552 939 360 387 533 3074
Fragments arranged by size:
3074 939 552 533 387 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3872 3889 4042 4227 4672 325
Size: 1255 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2229 2688
Size: 274 80 33 51 1443 153 99 459
Cuts at: 2688 2724 4091 5803 96
Size: 36 1367 1712 138
Fragments arranged by size:
1712 1443 1367 459 274 153 138 99
80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2574 2606 5805 5837 2574
Size: 32 3199 32 2582
Fragments arranged by size:
3199 2582 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2120 1316
Size: 804 5041
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 2297 1943
Size: 354 5491
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI DraIII EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII
SacII SfiI SfoI SrfI SwaI XcmI