(Circular) (Six-base) MAPSORT of: pFN21AE3139.seq Check: 2847 from: 1 to: 5755 pFN21AE3139 5755 bp With 182 enzymes: SgfI * March 28, 2012 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2780 2811 3565 278 Size: 53 83 186 936 1244 31 754 2468 Fragments arranged by size: 2468 1244 936 754 186 83 53 31 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3818 5202 5470 1632 Size: 2186 1384 268 1917 Fragments arranged by size: 2186 1917 1384 268 AfeI AGC'GCT Cuts at: 2606 2606 Size: 5755 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2220 4654 5631 1203 Size: 1017 2434 977 1327 Fragments arranged by size: 2434 1327 1017 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2240 1184 Size: 1056 4699 AlwNI CAG_nnn'CTG Cuts at: 2380 2751 2847 3498 5070 2380 Size: 371 96 651 1572 3065 Fragments arranged by size: 3065 1572 651 371 96 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2990 3064 3290 3442 5551 1493 Size: 1497 74 226 152 2109 1697 Fragments arranged by size: 2109 1697 1497 226 152 74 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2192 2485 2915 3080 1870 Size: 73 249 293 430 165 4545 Fragments arranged by size: 4545 430 293 249 165 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2380 2413 2380 Size: 33 5722 BamHI G'GATC_C Cuts at: 2625 3085 3452 2625 Size: 460 367 4928 Fragments arranged by size: 4928 460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 3076 4531 618 Size: 325 217 627 324 965 1455 1842 Fragments arranged by size: 1842 1455 965 627 325 324 217 BanII G_rGCy'C Cuts at: 1391 2922 3074 1391 Size: 1531 152 4072 Fragments arranged by size: 4072 1531 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2803 2989 928 Size: 971 904 186 3694 Fragments arranged by size: 3694 971 904 186 BbvCI CC'TCA_GC Cuts at: 2025 2840 2025 Size: 815 4940 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2408 3647 4857 2408 Size: 1239 1210 3306 Fragments arranged by size: 3306 1239 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4366 136 Size: 107 122 71 631 605 337 2357 1525 Fragments arranged by size: 2357 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2946 5750 2946 Size: 2804 2951 BlpI GC'TnA_GC Cuts at: 2672 3172 2672 Size: 500 5255 Bme1580I G_kGCm'C Cuts at: 1790 2345 2499 3816 4972 1790 Size: 555 154 1317 1156 2573 Fragments arranged by size: 2573 1317 1156 555 154 BmgBI CAC'GTC Cuts at: 1318 2512 2938 2964 1318 Size: 1194 426 26 4109 Fragments arranged by size: 4109 1194 426 26 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2437 2053 Size: 384 5371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2023 2489 4415 1239 Size: 784 466 1926 2579 Fragments arranged by size: 2579 1926 784 466 Bpu10I CC'TnA_GC Cuts at: 2025 2840 2025 Size: 815 4940 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2494 3546 493 Size: 711 1290 1052 2702 Fragments arranged by size: 2702 1290 1052 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2301 2777 2808 2886 Size: 53 83 186 936 768 476 31 78 Cuts at: 2886 3562 3944 4579 275 Size: 676 382 635 1451 Fragments arranged by size: 1451 936 768 676 635 476 382 186 83 78 53 31 BsaWI w'CCGG_w Cuts at: 2393 2580 4188 4586 4860 5007 5418 2393 Size: 187 1608 398 274 147 411 2730 Fragments arranged by size: 2730 1608 411 398 274 187 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 1178 Size: 30 165 30 1041 30 4459 Fragments arranged by size: 4459 1041 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2351 1186 Size: 856 309 4590 Fragments arranged by size: 4590 856 309 BseYI C'CCAG_C Cuts at: 2012 4958 5454 2012 Size: 2946 496 2313 Fragments arranged by size: 2946 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2271 2717 2927 1163 Size: 573 535 446 210 3991 Fragments arranged by size: 3991 573 535 446 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 2790 2922 3816 3901 4972 2135 Size: 655 132 894 85 1071 2918 Fragments arranged by size: 2918 1071 894 655 132 85 BsiWI C'GTAC_G Cuts at: 2245 2245 Size: 5755 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 BsmBI CGTCTCn'nnnn_ Cuts at: 2543 2543 Size: 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2345 2499 2790 2922 3074 3816 Size: 399 345 210 154 291 132 152 742 Cuts at: 3816 3901 4972 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 345 291 210 154 152 132 85 BspEI T'CCGG_A Cuts at: 2580 5418 2580 Size: 2838 2917 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2657 3110 844 Size: 1813 453 3489 Fragments arranged by size: 3489 1813 453 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2114 2888 4399 4586 1700 Size: 110 304 774 1511 187 2869 Fragments arranged by size: 2869 1511 774 304 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 2416 1925 Size: 491 5264 BssSI C'ACGA_G Cuts at: 2723 3815 4827 2723 Size: 1092 1012 3651 Fragments arranged by size: 3651 1092 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2512 794 Size: 1718 4037 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 2479 1184 Size: 1295 4460 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2047 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2569 2625 2946 3085 3452 3837 3854 5306 Size: 597 56 321 139 367 385 17 1452 Cuts at: 5306 5392 5404 5750 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 597 385 367 346 321 139 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2647 487 Size: 683 56 1421 3595 Fragments arranged by size: 3595 1421 683 56 BtsI GCAGTG_nn' Cuts at: 2629 3271 4065 4085 2629 Size: 642 794 20 4299 Fragments arranged by size: 4299 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3025 3060 529 Size: 35 2461 35 3224 Fragments arranged by size: 3224 2461 35 35 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 3202 4091 Size: 54 1425 50 275 18 408 964 889 Cuts at: 4091 5720 8 Size: 1629 43 Fragments arranged by size: 1629 1425 964 889 408 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 2178 2795 3056 3685 1353 Size: 770 55 617 261 629 3423 Fragments arranged by size: 3423 770 629 617 261 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2920 2920 Size: 5755 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2489 3818 4415 5202 5470 1239 Size: 393 391 466 1329 597 787 268 1524 Fragments arranged by size: 1524 1329 787 597 466 393 391 268 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 5755 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2476 2608 2672 2679 4902 1678 Size: 798 132 64 7 2223 2531 Fragments arranged by size: 2531 2223 798 132 64 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 4396 4428 4470 Size: 32 135 32 587 32 2205 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 2205 587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 2487 4869 5053 1305 Size: 1038 144 2382 184 2007 Fragments arranged by size: 2382 2007 1038 184 144 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 10 Size: 54 1425 343 408 3525 Fragments arranged by size: 3525 1425 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2903 3713 4072 4231 518 Size: 648 1737 810 359 159 2042 Fragments arranged by size: 2042 1737 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2377 3171 3848 4996 5241 1606 Size: 771 794 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 771 677 245 NaeI GCC'GGC Cuts at: 2890 2890 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2888 2888 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NruI TCG'CGA Cuts at: 2524 2524 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2722 2929 3113 4658 1132 Size: 97 1493 207 184 1545 2229 Fragments arranged by size: 2229 1545 1493 207 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5755 PfoI T'CCnGG_A Cuts at: 2123 2853 2123 Size: 730 5025 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2540 2572 3824 3856 5337 5369 2540 Size: 32 1252 32 1481 32 2926 Fragments arranged by size: 2926 1481 1252 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspXI vC'TCGA_Gb Cuts at: 2192 2915 2192 Size: 723 5032 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SacI G_AGCT'C Cuts at: 2922 2922 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SapI GCTCTTCn'nnn_ Cuts at: 2123 2795 2123 Size: 672 5083 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 3103 4238 4919 5110 826 Size: 219 2058 1135 681 191 1471 Fragments arranged by size: 2058 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 2487 3082 1872 Size: 615 595 4545 Fragments arranged by size: 4545 615 595 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 2915 3859 4760 5022 820 Size: 197 926 249 723 944 901 262 1553 Fragments arranged by size: 1553 944 926 901 723 262 249 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 2722 3113 1132 Size: 1590 391 3774 Fragments arranged by size: 3774 1590 391 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2405 1517 Size: 888 4867 StyI C'CwwG_G Cuts at: 513 1065 2004 2352 3194 513 Size: 552 939 348 842 3074 Fragments arranged by size: 3074 939 842 552 348 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2099 3782 3799 3952 4137 4582 325 Size: 1255 519 1683 17 153 185 445 1498 Fragments arranged by size: 1683 1498 1255 519 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2253 2285 5715 5747 2253 Size: 32 3430 32 2261 Fragments arranged by size: 3430 2261 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3005 1316 Size: 1689 4066 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 2192 2915 1943 Size: 249 723 4783 Fragments arranged by size: 4783 723 249 XmaI C'CCGG_G Cuts at: 1870 2485 3080 1870 Size: 615 595 4545 Fragments arranged by size: 4545 615 595 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2778 2809 3563 276 Size: 53 83 186 936 1244 31 754 2468 Fragments arranged by size: 2468 1244 936 754 186 83 53 31 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BfrBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI