################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BamHI BbeI BbrPI BcgI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaHI BsiWI BsmBI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MflI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 52 CGCG CG^CG AccIII 1166 TCCGGA T^CCGGA Alw44I 18 GTGCAC G^TGCAC AlwNI 887 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 18 GTGCAC G^TGCAC AvaII 131 GGACC G^G[AT]CC AvrII 1179 CCTAGG C^CTAGG BanI 1147 GGTGCC G^G[CT][GA]CC BbvCI 672 GCTGAGG CCTCAGC(-5/-2) BcnI 980 CCCGG CC^[GC]GG BglI 1194 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1179 CCTAGG C^CTAGG BsaBI 937 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 442 GAGACC GGTCTC(1/5) BsaWI 1166 TCCGGA [AT]^CCGG[AT] BseAI 1166 TCCGGA T^CCGGA BsgI 1118 GTGCAG GTGCAG(16/14) BsiEI 864 CGACCG CG[GA][CT]^CG BsmI 1170 GAATGC GAATGC(1/-1) BspEI 1166 TCCGGA T^CCGGA BstAPI 20 GCACTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 52 CGCG CG^CG CfoI 528 GCGC GCG^C DraII 1074 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1100 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 295 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 494 CCATGG C^C[GA][CT]GG EcoO109I 1074 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 229 GAATTC G^AATTC HaeII 527 AGCGCC [GA]GCGC^[CT] HhaI 528 GCGC GCG^C HinP1I 528 GCGC G^CGC HincII 862 GTCGAC GT[CT]^[GA]AC HindII 862 GTCGAC GT[CT]^[GA]AC MaeII 56 ACGT A^CGT MamI 937 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1166 TCCGGA T^CCGGA MvnI 52 CGCG CG^CG NciI 980 CCCGG CC^[GC]GG NcoI 494 CCATGG C^CATGG PvuII 586 CAGCTG CAG^CTG SalI 862 GTCGAC G^TCGAC SapI 183 GCTCTTC GCTCTTC(1/4) ScaI 419 AGTACT AGT^ACT SmlI 610 CTCAAG C^T[CT][GA]AG Tsp45I 1032 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 715 GTCTAC GT^[AC][GT]AC 862 GTCGAC AcsI 229 GAATTC [GA]^AATT[CT] 1273 AAATTC AocI 532 CCTGAGG CC^T[ACGT]AGG 650 CCTCAGG ApoI 229 GAATTC [GA]^AATT[CT] 1273 AAATTC AspI 250 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1291 GACACAGTC BalI 848 TGGCCA TGG^CCA 1107 TGGCCA BbsI 489 GAAGAC GAAGAC(2/6) 1356 GAAGAC BciVI 324 GGATAC GTATCC(6/5) 966 GGATAC BfaI 1024 CTAG C^TAG 1180 CTAG BpuAI 489 GAAGAC GAAGAC(2/6) 1356 GAAGAC BspHI 791 TCATGA T^CATGA 1298 TCATGA BspMI 133 ACCTGC ACCTGC(4/8) 836 ACCTGC BsrDI 1310 CATTGC GCAATG(2/0) 341 GCAATG BsrFI 470 GCCGGC [GA]^CCGG[CT] 917 GCCGGC Bsu36I 532 CCTGAGG CC^T[ACGT]AGG 650 CCTCAGG Cfr10I 470 GCCGGC [GA]^CCGG[CT] 917 GCCGGC EarI 184 CTCTTC CTCTTC(1/4) 1372 CTCTTC Eco81I 532 CCTGAGG CC^T[ACGT]AGG 650 CCTCAGG Ksp632I 184 CTCTTC CTCTTC(1/4) 1372 CTCTTC MaeI 1024 CTAG C^TAG 1180 CTAG MaeIII 1004 GTTAC ^GT[ACGT]AC 1032 GTCAC MluNI 848 TGGCCA TGG^CCA 1107 TGGCCA MscI 848 TGGCCA TGG^CCA 1107 TGGCCA MslI 558 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1293 CACAGTCATG MspA1I 575 CAGCGG C[AC]G^C[GT]G 586 CAGCTG NaeI 470 GCCGGC GCC^GGC 917 GCCGGC NgoMIV 470 GCCGGC G^CCGGC 917 GCCGGC NlaIV 1075 GGCCCC GG[ACGT]^[ACGT]CC 1147 GGTGCC PflFI 250 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1291 GACACAGTC PleI 1087 GAGTC GAGTC(4/5) 1213 GAGTC PstI 361 CTGCAG CTGCA^G 820 CTGCAG RcaI 791 TCATGA T^CATGA 1298 TCATGA SfcI 361 CTGCAG C^T[GA][CT]AG 820 CTGCAG StuI 368 AGGCCT AGG^CCT 647 AGGCCT Tth111I 250 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1291 GACACAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I