Restriction map for
pFN21AE1930
(Circular) (Six-base) MAPSORT of: pFN21AE1930.seq Check: 9791 from: 1 to: 6112
pFN21AE1930 6112 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 2868 3455 1937
Size: 784 147 587 4594
Fragments arranged by size:
4594 784 587 147
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2774 2933 4175 5559 5827 1632
Size: 1142 159 1242 1384 268 1917
Fragments arranged by size:
1917 1384 1242 1142 268 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2897 3855 5427 2897
Size: 958 1572 3582
Fragments arranged by size:
3582 1572 958
ApaLI G'TGCA_C
Cuts at: 2023 4169 5325 2023
Size: 2146 1156 2810
Fragments arranged by size:
2810 2146 1156
ApoI r'AATT_y
Cuts at: 1493 2234 3278 3421 3647 3799 5908 1493
Size: 741 1044 143 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 741 226 152 143
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
AvrII C'CTAG_G
Cuts at: 3184 3184
Size: 6112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2343 2376 2343
Size: 33 6079
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3152 3433 4888 618
Size: 325 217 627 1365 281 1455 1842
Fragments arranged by size:
1842 1455 1365 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2269 2891 3264 3431 1391
Size: 878 622 373 167 4072
Fragments arranged by size:
4072 878 622 373 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 3368 928
Size: 971 602 867 3672
Fragments arranged by size:
3672 971 867 602
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 6112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4303 4337 1903
Size: 34 50 34 2282 34 3678
Fragments arranged by size:
3678 2282 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2323 2965 4004 5214 2323
Size: 642 1039 1210 3221
Fragments arranged by size:
3221 1210 1039 642
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3205 4723 136
Size: 107 122 71 631 605 1533 1518 1525
Fragments arranged by size:
1533 1525 1518 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 2027 4173 5329 1790
Size: 237 2146 1156 2573
Fragments arranged by size:
2573 2146 1156 237
BmgBI CAC'GTC
Cuts at: 1318 2062 1318
Size: 744 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3116 3843 4801
Size: 416 53 83 399 24 1699 727 958
Cuts at: 4801 442
Size: 1753
Fragments arranged by size:
1753 1699 958 727 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 2683 2977 4772 1239
Size: 1268 176 294 1795 2579
Fragments arranged by size:
2579 1795 1268 294 176
Bpu10I CC'TnA_GC
Cuts at: 2480 2678 2480
Size: 198 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2600 4237 5102 5400 2600
Size: 1637 865 298 3312
Fragments arranged by size:
3312 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2441 4775 882
Size: 617 942 2334 2219
Fragments arranged by size:
2334 2219 942 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 2946 6106 2946
Size: 3160 2952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3171 4545 4943 5217 5364 5775 3171
Size: 1374 398 274 147 411 3508
Fragments arranged by size:
3508 1374 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5887
Fragments arranged by size:
5887 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2228 2480 2561 1186
Size: 1039 3 252 81 4737
Fragments arranged by size:
4737 1039 252 81 3
BseYI C'CCAG_C
Cuts at: 2268 2890 2980 3000 3096 5315 5811 2268
Size: 622 90 20 96 2219 496 2569
Fragments arranged by size:
2569 2219 622 496 96 90 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3144 1163
Size: 573 1408 4131
Fragments arranged by size:
4131 1408 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3562 4323 5351 1540
Size: 275 186 871 690 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 690 275 186
BsiHKAI G_wGCw'C
Cuts at: 2020 2027 2402 4173 4258 5329 2020
Size: 7 375 1771 85 1071 2803
Fragments arranged by size:
2803 1771 1071 375 85 7
BsmI GAATG_Cn'
Cuts at: 3181 3623 3716 3181
Size: 442 93 5577
Fragments arranged by size:
5577 442 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2027 2269 2402 2891 3264 3431
Size: 399 230 7 242 133 489 373 167
Cuts at: 3431 4173 4258 5329 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 373 242 230
167 133 85 7
BspEI T'CCGG_A
Cuts at: 3171 5775 3171
Size: 2604 3508
BspHI T'CATG_A
Cuts at: 2796 3303 4001 5731 2796
Size: 507 698 1730 3177
Fragments arranged by size:
3177 1730 698 507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 2850 3467 844
Size: 1303 703 617 3489
Fragments arranged by size:
3489 1303 703 617
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2353 3314 4607 4781 65
Size: 1183 1105 961 1293 174 1396
Fragments arranged by size:
1396 1293 1183 1105 961 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2475 2922 4756 4943 1700
Size: 110 665 447 1834 187 2869
Fragments arranged by size:
2869 1834 665 447 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2031 794
Size: 1237 4875
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2538 2656 2538
Size: 118 5994
BtgI C'CryG_G
Cuts at: 513 1065 2499 513
Size: 552 1434 4126
Fragments arranged by size:
4126 1434 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2105 3628 4422 4442 2074
Size: 31 1523 794 20 3744
Fragments arranged by size:
3744 1523 794 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DraIII CAC_nnn'GTG
Cuts at: 3110 3110
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2306 5119 809
Size: 507 990 2813 1802
Fragments arranged by size:
2813 1802 990 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2080 2853
Size: 54 1425 50 275 18 234 16 773
Cuts at: 2853 2924 3112 3268 3559 4448 6077 8
Size: 71 188 156 291 889 1629 43
Fragments arranged by size:
1629 1425 889 773 291 275 234 188
156 71 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 3383 4042 1353
Size: 842 1188 659 3423
Fragments arranged by size:
3423 1188 842 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2909 3299 4699 5073 5219 387
Size: 1064 138 1320 390 1400 374 146 1280
Fragments arranged by size:
1400 1320 1280 1064 390 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 2683 2774 2933 2977 4175 4772
Size: 393 875 176 91 159 44 1198 597
Cuts at: 4772 5559 5827 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1198 875 787 597 393 268 176
159 91 44
EcoO109I rG'GnC_Cy
Cuts at: 3080 3080
Size: 6112
EcoRI G'AATT_C
Cuts at: 2234 3421 3799 2234
Size: 1187 378 4547
Fragments arranged by size:
4547 1187 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2536 5259 1678
Size: 858 2723 2531
Fragments arranged by size:
2723 2531 858
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373
Size: 32 135 32 3181 32 42 32 2626
Fragments arranged by size:
3181 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 3456 3702 669
Size: 988 281 931 587 246 3079
Fragments arranged by size:
3079 988 931 587 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2114 5226 5410 1305
Size: 756 53 3112 184 2007
Fragments arranged by size:
3112 2007 756 184 53
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2855 3114 10
Size: 54 1425 343 1023 259 3008
Fragments arranged by size:
3008 1425 1023 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3302 4070 4429 4588 518
Size: 648 1401 735 768 359 159 2042
Fragments arranged by size:
2042 1401 768 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2582 2593 3528 4205 5353 5598 1606
Size: 976 11 935 677 1148 245 2120
Fragments arranged by size:
2120 1148 976 935 677 245 11
NaeI GCC'GGC
Cuts at: 2477 2924 2477
Size: 447 5665
NcoI C'CATG_G
Cuts at: 513 1065 2499 513
Size: 552 1434 4126
Fragments arranged by size:
4126 1434 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NgoMIV G'CCGG_C
Cuts at: 2475 2922 2475
Size: 447 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 3470 5015 1132
Size: 97 2241 1545 2229
Fragments arranged by size:
2241 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PstI C_TGCA'G
Cuts at: 830 2370 2829 3464 830
Size: 1540 459 635 3478
Fragments arranged by size:
3478 1540 635 459
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2593 2593
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 2867 3454 1936
Size: 931 587 4594
Fragments arranged by size:
4594 931 587
SapI GCTCTTCn'nnn_
Cuts at: 2195 2195
Size: 6112
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 2426 4360 6072 2426
Size: 1934 1712 2466
Fragments arranged by size:
2466 1934 1712
SfcI C'TryA_G
Cuts at: 826 1045 2366 2825 3460 4595 5276 5467 826
Size: 219 1321 459 635 1135 681 191 1471
Fragments arranged by size:
1471 1321 1135 681 635 459 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2615 4216 5117 5379 820
Size: 197 926 672 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 672 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 2654 1517
Size: 858 279 4975
Fragments arranged by size:
4975 858 279
StyI C'CwwG_G
Cuts at: 513 1065 2376 2499 3184 3336 3551 513
Size: 552 1311 123 685 152 215 3074
Fragments arranged by size:
3074 1311 685 552 215 152 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2555 3098 4139 4156 4309 4494 4939
Size: 1255 975 543 1041 17 153 185 445
Cuts at: 4939 325
Size: 1498
Fragments arranged by size:
1498 1255 1041 975 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2601 4358
Size: 274 80 33 51 1443 447 177 1757
Cuts at: 4358 6070 96
Size: 1712 138
Fragments arranged by size:
1757 1712 1443 447 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 6080
Tth111I GACn'n_nGTC
Cuts at: 1316 2258 3299 1316
Size: 942 1041 4129
Fragments arranged by size:
4129 1041 942
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI