Restriction map for
pFN21AE6532
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI MaeI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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ApaI 77 GGGCCC GGGCC^C
AspHI 223 GTGCTC G[AT]GC[AT]^C
AvaII 365 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BamHI 274 GGATCC G^GATCC
BbsI 295 GAAGAC GAAGAC(2/6)
BcnI 329 CCCGG CC^[GC]GG
BglI 160 GCCGCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 295 GAAGAC GAAGAC(2/6)
BsaAI 20 TACGTA [CT]AC^GT[GA]
BsaBI 333 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 359 GAGACC GGTCTC(1/5)
BsaWI 362 ACCGGA [AT]^CCGG[AT]
BsiHKAI 223 GTGCTC G[AT]GC[AT]^C
BsmAI 359 GAGAC GTCTC(1/5)
Bsp1407I 173 TGTACA T^GTACA
BsrFI 105 GCCGGC [GA]^CCGG[CT]
BsrGI 173 TGTACA T^GTACA
BstYI 274 GGATCC [GA]^GATC[CT]
Cfr10I 105 GCCGGC [GA]^CCGG[CT]
CpoI 364 CGGACCG CG^G[AT]CCG
DdeI 164 CTCAG C^T[ACGT]AG
DraII 77 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 250 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 310 CTCTTC CTCTTC(1/4)
EcoNI 505 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 77 GGGCCCC [GA]G^G[ACGT]CC[CT]
HgaI 132 GACGC GACGC(5/10)
HphI 290 TCACC GGTGA(8/7)
Ksp632I 310 CTCTTC CTCTTC(1/4)
MaeIII 484 GTAAC ^GT[ACGT]AC
MamI 333 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 274 GGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 414 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 105 GCCGGC GCC^GGC
NciI 329 CCCGG CC^[GC]GG
NgoMIV 105 GCCGGC G^CCGGC
PstI 376 CTGCAG CTGCA^G
RsrII 364 CGGACCG CG^G[AT]CCG
SfcI 376 CTGCAG C^T[GA][CT]AG
SgrAI 104 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 20 TACGTA TAC^GTA
SspBI 173 TGTACA T^GTACA
TaqI 179 TCGA T^CGA
TspRI 262 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 179 TCGA T^CGA
XhoII 274 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 30 GTAC GT^AC
174 GTAC
AlwI 275 GATCC GGATC(4/5)
274 GGATC
BanII 77 GGGCCC G[GA]GC[CT]^C
325 GAGCCC
BsgI 259 GTGCAG GTGCAG(16/14)
427 GTGCAG
BspHI 203 TCATGA T^CATGA
471 TCATGA
BsrBI 8 CCGCTC CCGCTC(-3/-3)
161 CCGCTC
BsrDI 414 CATTGC GCAATG(2/0)
169 GCAATG
BsrI 355 ACTGG ACTGG(1/-1)
487 ACTGG
BstF5I 501 CATCC GGATG(2/0)
111 GGATG
Cac8I 105 GCCGGC GC[ACGT]^[ACGT]GC
221 GCGTGC
DpnII 275 GATC ^GATC
339 GATC
DsaI 82 CCGCGG C^C[GA][CT]GG
195 CCGCGG
FokI 501 CATCC GGATG(9/13)
111 GGATG
KspI 82 CCGCGG CCGC^GG
195 CCGCGG
MaeII 21 ACGT A^CGT
269 ACGT
MboI 275 GATC ^GATC
339 GATC
MboII 295 GAAGA GAAGA(8/7)
311 TCTTC
NdeII 275 GATC ^GATC
339 GATC
RcaI 203 TCATGA T^CATGA
471 TCATGA
RsaI 30 GTAC GT^AC
174 GTAC
SacII 82 CCGCGG CCGC^GG
195 CCGCGG
Sau3AI 275 GATC ^GATC
339 GATC
SfaNI 93 GCATC GCATC(5/9)
137 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstUI CfoI
Cfr13I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI