Restriction map for
pFN21AE6532
(Circular) (Six-base) MAPSORT of: pFN21AE6532.seq Check: 9731 from: 1 to: 5257
pFN21AE6532 5257 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3067 278
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2600 1937
Size: 663 4594
Acc65I G'GTAC_C
Cuts at: 2578 2578
Size: 5257
AclI AA'CG_TT
Cuts at: 1563 3384 3757 1563
Size: 1821 373 3063
Fragments arranged by size:
3063 1821 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3320 4704 4972 1632
Size: 1688 1384 268 1917
Fragments arranged by size:
1917 1688 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5060
AflIII A'CryG_T
Cuts at: 1203 4156 5133 1203
Size: 2953 977 1327
Fragments arranged by size:
2953 1327 977
AgeI A'CCGG_T
Cuts at: 4088 4088
Size: 5257
AhdI GACnn_n'nnGTC
Cuts at: 3986 3986
Size: 5257
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2165 1184
Size: 981 4276
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 2264
Size: 32 5225
AlwNI CAG_nnn'CTG
Cuts at: 3000 4572 3000
Size: 1572 3685
ApaI G_GGCC'C
Cuts at: 2086 2086
Size: 5257
ApaLI G'TGCA_C
Cuts at: 3314 4470 3314
Size: 1156 4101
ApoI r'AATT_y
Cuts at: 1493 2566 2792 2944 5053 1493
Size: 1073 226 152 2109 1697
Fragments arranged by size:
2109 1697 1073 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3811 160
Size: 872 2779 1606
Fragments arranged by size:
2779 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
AvaI C'yCGr_G
Cuts at: 1870 1943 2582 1870
Size: 73 639 4545
Fragments arranged by size:
4545 639 73
BamHI G'GATC_C
Cuts at: 2279 2587 2954 2279
Size: 308 367 4582
Fragments arranged by size:
4582 367 308
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2578 4033 618
Size: 325 217 627 791 1455 1842
Fragments arranged by size:
1842 1455 791 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2086 2334 2576 1391
Size: 695 248 242 4072
Fragments arranged by size:
4072 695 248 242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 928
Size: 971 408 3878
Fragments arranged by size:
3878 971 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3448 3482 1903
Size: 34 1511 34 3678
Fragments arranged by size:
3678 1511 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 4359 3149
Size: 1210 4047
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 3868 136
Size: 107 122 71 631 605 499 1697 1525
Fragments arranged by size:
1697 1525 631 605 499 122 107 71
BglII A'GATC_T
Cuts at: 5252 5252
Size: 5257
BlpI GC'TnA_GC
Cuts at: 2674 2674
Size: 5257
Bme1580I G_kGCm'C
Cuts at: 1790 2086 3318 4474 1790
Size: 296 1232 1156 2573
Fragments arranged by size:
2573 1232 1156 296
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2988 3946 442
Size: 416 53 83 399 24 1571 958 1753
Fragments arranged by size:
1753 1571 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2519
Size: 5257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2382 2514 3917 1239
Size: 1109 34 132 1403 2579
Fragments arranged by size:
2579 1403 1109 132 34
Bpu10I CC'TnA_GC
Cuts at: 2169 2169
Size: 5257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2538 3382 4247 4545 2538
Size: 844 865 298 3250
Fragments arranged by size:
3250 865 844 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2358 3920 882
Size: 617 859 1562 2219
Fragments arranged by size:
2219 1562 859 617
BsaAI yAC'GTr
Cuts at: 493 1204 2027 3048 493
Size: 711 823 1021 2702
Fragments arranged by size:
2702 1021 823 711
BsaBI GATnn'nnATC
Cuts at: 2342 5251 2342
Size: 2909 2348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3064 3446 4081 275
Size: 53 83 186 936 1531 382 635 1451
Fragments arranged by size:
1531 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2367 3690 4088 4362 4509 4920 2367
Size: 1323 398 274 147 411 2704
Fragments arranged by size:
2704 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5032
Fragments arranged by size:
5032 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5257
BseYI C'CCAG_C
Cuts at: 4460 4956 4460
Size: 496 4761
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2453 1163
Size: 573 549 168 3967
Fragments arranged by size:
3967 573 549 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 3468 4496 1540
Size: 275 186 706 761 1028 2301
Fragments arranged by size:
2301 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 3318 3403 4474 2232
Size: 1086 85 1071 3015
Fragments arranged by size:
3015 1086 1071 85
BsmI GAATG_Cn'
Cuts at: 2768 2861 2768
Size: 93 5164
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2232 2334 2576 3318 3403 4474
Size: 399 296 146 102 242 742 85 1071
Cuts at: 4474 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 296 242 146 102
85
BspEI T'CCGG_A
Cuts at: 4920 4920
Size: 5257
BspHI T'CATG_A
Cuts at: 2208 2476 3146 4876 2208
Size: 268 670 1730 2589
Fragments arranged by size:
2589 1730 670 268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2612 844
Size: 1768 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 2168 3144 1394
Size: 429 192 153 976 3507
Fragments arranged by size:
3507 976 429 192 153
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 2418 3752 3926 65
Size: 1183 933 237 1334 174 1396
Fragments arranged by size:
1396 1334 1183 933 237 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 3901 4088 1700
Size: 110 300 1791 187 2869
Fragments arranged by size:
2869 1791 300 187 110
BsrGI T'GTAC_A
Cuts at: 96 2178 96
Size: 2082 3175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5257
BssSI C'ACGA_G
Cuts at: 3317 4329 3317
Size: 1012 4245
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5257
BstBI TT'CG_AA
Cuts at: 4061 4061
Size: 5257
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5257
BstYI r'GATC_y
Cuts at: 1972 2279 2587 2954 3339 3356 4808 4894 4906
Size: 307 308 367 385 17 1452 86 12
Cuts at: 4906 5252 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 308 307 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2087 2200 513
Size: 552 939 83 113 3570
Fragments arranged by size:
3570 939 552 113 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4518
Fragments arranged by size:
4518 683 56
BtsI GCAGTG_nn'
Cuts at: 2273 2773 3567 3587 2273
Size: 500 794 20 3943
Fragments arranged by size:
3943 794 500 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5222
DraI TTT'AAA
Cuts at: 2561 2561
Size: 5257
DraIII CAC_nnn'GTG
Cuts at: 2260 2260
Size: 5257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4264 809
Size: 507 2948 1802
Fragments arranged by size:
2948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2704 3593
Size: 54 1425 50 275 18 177 697 889
Cuts at: 3593 5222 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 697 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2704 1537
Size: 275 892 4090
Fragments arranged by size:
4090 892 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 3187 1353
Size: 968 866 3423
Fragments arranged by size:
3423 968 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2052 2129 3844 4218 4364 387
Size: 1064 138 463 77 1715 374 146 1280
Fragments arranged by size:
1715 1280 1064 463 374 146 138 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2382 2514 3320 3917 4704 4972
Size: 393 716 34 132 806 597 787 268
Cuts at: 4972 1239
Size: 1524
Fragments arranged by size:
1524 806 787 716 597 393 268 132
34
EcoNI CCTnn'n_nnAGG
Cuts at: 2514 2514
Size: 5257
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 5257
EcoRI G'AATT_C
Cuts at: 2566 2944 2566
Size: 378 4879
FspI TGC'GCA
Cuts at: 3763 3763
Size: 5257
HaeII r_GCGC'y
Cuts at: 1678 4404 1678
Size: 2726 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3898 3930 3972 4004 1373
Size: 32 135 32 2326 32 42 32 2626
Fragments arranged by size:
2626 2326 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2601 2847 669
Size: 988 281 663 246 3079
Fragments arranged by size:
3079 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5257
HpaI GTT'AAC
Cuts at: 2847 2847
Size: 5257
KpnI G_GTAC'C
Cuts at: 2582 2582
Size: 5257
MfeI C'AATT_G
Cuts at: 2856 2856
Size: 5257
MluI A'CGCG_T
Cuts at: 5133 5133
Size: 5257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4371 4555 1305
Size: 3066 184 2007
Fragments arranged by size:
3066 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3258
Fragments arranged by size:
3258 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3215 3574 3733 518
Size: 648 1257 792 359 159 2042
Fragments arranged by size:
2042 1257 792 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2134 2202 2413 2673 3350 4498 4743
Size: 483 45 68 211 260 677 1148 245
Cuts at: 4743 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 483 260 245 211 68
45
NaeI GCC'GGC
Cuts at: 2112 2112
Size: 5257
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3766
Fragments arranged by size:
3766 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5257
NgoMIV G'CCGG_C
Cuts at: 2110 2110
Size: 5257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5257
NotI GC'GGCC_GC
Cuts at: 2704 2704
Size: 5257
NspI r_CATG'y
Cuts at: 1132 1229 2615 4160 1132
Size: 97 1386 1545 2229
Fragments arranged by size:
2229 1545 1386 97
PasI CC'CwG_GG
Cuts at: 1348 2079 1348
Size: 731 4526
PciI A'CATG_T
Cuts at: 4156 4156
Size: 5257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5257
PmeI GTTT'AAAC
Cuts at: 2561 2561
Size: 5257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 4839 4871 3326
Size: 32 1481 32 3712
Fragments arranged by size:
3712 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5257
PsiI TTA'TAA
Cuts at: 2827 2827
Size: 5257
PspOMI G'GGCC_C
Cuts at: 2082 2082
Size: 5257
PstI C_TGCA'G
Cuts at: 830 2385 2609 830
Size: 1555 224 3478
Fragments arranged by size:
3478 1555 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5257
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 5257
SacII CC_GC'GG
Cuts at: 2090 2203 2090
Size: 113 5144
SalI G'TCGA_C
Cuts at: 1936 2599 1936
Size: 663 4594
SbfI CC_TGCA'GG
Cuts at: 2609 2609
Size: 5257
ScaI AGT'ACT
Cuts at: 3505 5217 3505
Size: 1712 3545
SfcI C'TryA_G
Cuts at: 826 1045 2381 2605 3740 4421 4612 826
Size: 219 1336 224 1135 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 224 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
SgrAI Cr'CCGG_yG
Cuts at: 1700 2110 1700
Size: 410 4847
SmaI CCC'GGG
Cuts at: 1872 2584 1872
Size: 712 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2553 3361 4262 4524 820
Size: 197 926 610 808 901 262 1553
Fragments arranged by size:
1553 926 901 808 610 262 197
SnaBI TAC'GTA
Cuts at: 493 2027 3048 493
Size: 1534 1021 2702
Fragments arranged by size:
2702 1534 1021
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5257
SphI G_CATG'C
Cuts at: 1132 2615 1132
Size: 1483 3774
SspI AAT'ATT
Cuts at: 5 52 3181 5
Size: 47 3129 2081
Fragments arranged by size:
3129 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5257
StyI C'CwwG_G
Cuts at: 513 1065 2004 2696 513
Size: 552 939 692 3074
Fragments arranged by size:
3074 939 692 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2387 3284 3301 3454 3639 4084 325
Size: 1255 807 897 17 153 185 445 1498
Fragments arranged by size:
1498 1255 897 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3503 5215
Size: 274 80 33 51 1443 201 1325 1712
Cuts at: 5215 96
Size: 138
Fragments arranged by size:
1712 1443 1325 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 5217 5249 2211
Size: 32 2974 32 2219
Fragments arranged by size:
2974 2219 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5257
XbaI T'CTAG_A
Cuts at: 2593 2593
Size: 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5257
XmaI C'CCGG_G
Cuts at: 1870 2582 1870
Size: 712 4545
XmnI GAAnn'nnTTC
Cuts at: 3386 3386
Size: 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3065 276
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI