################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 416 TCCGGA T^CCGGA AfaI 493 GTAC GT^AC AspHI 46 GTGCTC G[AT]GC[AT]^C AspI 501 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 354 GGGCTC G[GA]GC[CT]^C BbsI 3 GAAGAC GAAGAC(2/6) BbvCI 96 CCTCAGC CCTCAGC(-5/-2) BbvI 54 GCTGC GCAGC(8/12) BpuAI 3 GAAGAC GAAGAC(2/6) BsaWI 416 TCCGGA [AT]^CCGG[AT] BseAI 416 TCCGGA T^CCGGA BsiHKAI 46 GTGCTC G[AT]GC[AT]^C BsmAI 247 GAGAC GTCTC(1/5) BspEI 416 TCCGGA T^CCGGA BsrDI 84 CATTGC GCAATG(2/0) BsrI 42 ACTGG ACTGG(1/-1) BstXI 9 CCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 97 CTCAG C^T[ACGT]AG DraI 448 TTTAAA TTT^AAA DrdI 267 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 339 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 54 GCTGC GC^[ACGT]GC HapII 417 CCGG C^CGG HpaII 417 CCGG C^CGG ItaI 54 GCTGC GC^[ACGT]GC MroI 416 TCCGGA T^CCGGA MseI 449 TTAA T^TAA MspA1I 142 CAGCGG C[AC]G^C[GT]G MspI 417 CCGG C^CGG PflFI 501 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 21 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 505 GAGTC GAGTC(4/5) RsaI 493 GTAC GT^AC SfcI 125 CTATAG C^T[GA][CT]AG Tru9I 449 TTAA T^TAA TseI 54 GCTGC G^C[AT]GC Tsp509I 456 AATT ^AATT Tth111I 501 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 21 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 188 CCAGGCCCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BalI 18 TGGCCA TGG^CCA 77 TGGCCA BsmFI 206 GGGAC GGGAC(10/14) 328 GGGAC DraII 92 AGGTCCT [GA]G^G[ACGT]CC[CT] 206 GGGACCC EaeI 18 TGGCCA [CT]^GGCC[GA] 77 TGGCCA EcoO109I 92 AGGTCCT [GA]G^G[ACGT]CC[CT] 206 GGGACCC MluNI 18 TGGCCA TGG^CCA 77 TGGCCA MscI 18 TGGCCA TGG^CCA 77 TGGCCA PpuMI 92 AGGTCCT [GA]G^G[AT]CC[CT] 206 GGGACCC TfiI 467 GAATC G^A[AT]TC 486 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI AvaII BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I EcoRII FokI HaeIII HinfI HphI MaeIII MboII MnlI MvaI MwoI NlaIV Sau96I ScrFI SfaNI Tsp45I TspRI