(Linear) (Six-base) MAPSORT of: KIEE1653.seq  Check: 2521  from: 1  to: 510



KIEE1653.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

BanII G_rGCy'C

Cuts at:      0     358     510 
   Size:        358     152
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     510 
   Size:         10     500
 
BbvCI CC'TCA_GC

Cuts at:      0      97     510 
   Size:         97     413
 
Bme1580I G_kGCm'C

Cuts at:      0     259     510 
   Size:        259     251
 
Bpu10I CC'TnA_GC

Cuts at:      0      97     510 
   Size:         97     413
 
BsaWI w'CCGG_w

Cuts at:      0     416     510 
   Size:        416      94
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      32      62     238     268     510 
   Size:         32      30     176      30     242

  Fragments arranged by size: 

                 242     176      32      30      30
 
BseYI C'CCAG_C

Cuts at:      0      57     402     510 
   Size:         57     345     108

  Fragments arranged by size: 

                 345     108      57
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     510 
   Size:         50     460
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     259     358     510 
   Size:         50     209      99     152

  Fragments arranged by size: 

                 209     152      99      50
 
BspEI T'CCGG_A

Cuts at:      0     416     510 
   Size:        416      94
 
BsrDI GCAATG_nn'

Cuts at:      0      83     510 
   Size:         83     427
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      16     510 
   Size:         16     494
 
DraI TTT'AAA

Cuts at:      0     450     510 
   Size:        450      60
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     273     510 
   Size:        273     237
 
EaeI y'GGCC_r

Cuts at:      0      18      77     510 
   Size:         18      59     433

  Fragments arranged by size: 

                 433      59      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     343     510 
   Size:        343     167
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     207     510 
   Size:         93     114     303

  Fragments arranged by size: 

                 303     114      93
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     133     510 
   Size:        133     377
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     510 
   Size:          7      32     471

  Fragments arranged by size: 

                 471      32       7
 
MscI TGG'CCA

Cuts at:      0      20      79     510 
   Size:         20      59     431

  Fragments arranged by size: 

                 431      59      20
 
MspA1I CmG'CkG

Cuts at:      0     144     510 
   Size:        144     366
 
PasI CC'CwG_GG

Cuts at:      0      27     510 
   Size:         27     483
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      27     510 
   Size:         27     483
 
PfoI T'CCnGG_A

Cuts at:      0     173     510 
   Size:        173     337
 
PpuMI rG'GwC_Cy

Cuts at:      0      93     207     510 
   Size:         93     114     303

  Fragments arranged by size: 

                 303     114      93
 
SanDI GG'GwC_CC

Cuts at:      0     207     510 
   Size:        207     303
 
SfcI C'TryA_G

Cuts at:      0     125     510 
   Size:        125     385
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     394     510 
   Size:        394     116
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64     238     270     510 
   Size:         32      32     174      32     240

  Fragments arranged by size: 

                 240     174      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     504     510 
   Size:        504       6
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195     510 
   Size:        195     315
 

 Enzymes that do cut:

    BanII     BbsI    BbvCI Bme1580I   Bpu10I    BsaWI    BsaXI    BseYI
  BsiHKAI Bsp1286I    BspEI    BsrDI    BstXI     DraI     DrdI     EaeI
    EcoNI EcoO109I     FalI    Hin4I     MscI   MspA1I     PasI    PflMI
     PfoI    PpuMI    SanDI     SfcI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     XbaI
     XhoI     XmaI     XmnI     ZraI