################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeIII MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AcsI 103 GAATTT [GA]^AATT[CT] AfaI 512 GTAC GT^AC AlwI 44 GGATC GGATC(4/5) AlwNI 237 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 424 CCTCAGG CC^T[ACGT]AGG ApoI 103 GAATTT [GA]^AATT[CT] AvaI 119 CTCGGG C^[CT]CG[GA]G AvaII 275 GGTCC G^G[AT]CC AviII 329 TGCGCA TGC^GCA BalI 50 TGGCCA TGG^CCA BbsI 186 GTCTTC GAAGAC(2/6) BfaI 529 CTAG C^TAG BpmI 488 CTCCAG CTGGAG(16/14) BpuAI 186 GTCTTC GAAGAC(2/6) BsaAI 509 CACGTA [CT]AC^GT[GA] BsaBI 39 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 163 GAGGAG GAGGAG(10/8) BsgI 506 CTGCAC GTGCAG(16/14) BsoBI 119 CTCGGG C^[CT]CG[GA]G BsrI 72 ACTGG ACTGG(1/-1) BssSI 472 CTCGTG CACGAG(-5/-1) BstAPI 321 GCACGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 462 GGATG GGATG(2/0) Bsu36I 424 CCTCAGG CC^T[ACGT]AGG CfoI 330 GCGC GCG^C DdeI 425 CTCAG C^T[ACGT]AG DpnII 45 GATC ^GATC DraII 274 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 206 CCATGG C^C[GA][CT]GG EaeI 50 TGGCCA [CT]^GGCC[GA] Eco81I 424 CCTCAGG CC^T[ACGT]AGG EcoO109I 274 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 206 CCATGG C^C[AT][AT]GG FauI 20 CCCGC CCCGC(4/6) FokI 462 GGATG GGATG(9/13) FspI 329 TGCGCA TGC^GCA HhaI 330 GCGC GCG^C HinP1I 330 GCGC G^CGC HinfI 486 GACTC G^A[ACGT]TC MaeI 529 CTAG C^TAG MaeII 510 ACGT A^CGT MamI 39 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 45 GATC ^GATC MluNI 50 TGGCCA TGG^CCA MscI 50 TGGCCA TGG^CCA MslI 519 CACTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 414 CCGCTG C[AC]G^C[GT]G NcoI 206 CCATGG C^CATGG NdeII 45 GATC ^GATC NspI 266 GCATGC [GA]CATG^[CT] PleI 486 GACTC GAGTC(4/5) PpuMI 274 GGGTCCT [GA]G^G[AT]CC[CT] PstI 6 CTGCAG CTGCA^G RsaI 512 GTAC GT^AC Sau3AI 45 GATC ^GATC SfaNI 536 GCATC GCATC(5/9) SfcI 6 CTGCAG C^T[GA][CT]AG SphI 266 GCATGC GCATG^C StyI 206 CCATGG C^C[AT][AT]GG TspRI 33 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AspHI 319 GAGCAC G[AT]GC[AT]^C 466 GAGCAC BciVI 124 GGATAC GTATCC(6/5) 212 GGATAC BcnI 86 CCGGG CC^[GC]GG 439 CCCGG BsiHKAI 319 GAGCAC G[AT]GC[AT]^C 466 GAGCAC BspMI 241 ACCTGC ACCTGC(4/8) 262 ACCTGC DraIII 322 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG 469 CACCTCGTG HapII 86 CCGG C^CGG 440 CCGG HpaII 86 CCGG C^CGG 440 CCGG MboII 12 GAAGA GAAGA(8/7) 187 TCTTC MspI 86 CCGG C^CGG 440 CCGG NciI 86 CCGGG CC^[GC]GG 439 CCCGG Tsp509I 104 AATT ^AATT 174 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI