################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI AluI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TseI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 48 GCGG CCGC(-3/-1) AcsI 362 GAATTC [GA]^AATT[CT] AfaI 535 GTAC GT^AC AflII 630 CTTAAG C^TTAAG AocI 578 CCTCAGG CC^T[ACGT]AGG ApaI 490 GGGCCC GGGCC^C ApoI 362 GAATTC [GA]^AATT[CT] AspHI 162 GTGCTC G[AT]GC[AT]^C BamHI 338 GGATCC G^GATCC BanII 490 GGGCCC G[GA]GC[CT]^C BclI 416 TGATCA T^GATCA BfrI 630 CTTAAG C^TTAAG BpmI 222 CTGGAG CTGGAG(16/14) BsaI 81 GAGACC GGTCTC(1/5) BsiHKAI 162 GTGCTC G[AT]GC[AT]^C BsmAI 81 GAGAC GTCTC(1/5) BsmFI 8 GGGAC GGGAC(10/14) BstAPI 635 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 125 GGATG GGATG(2/0) BstYI 338 GGATCC [GA]^GATC[CT] Bsu36I 578 CCTCAGG CC^T[ACGT]AGG Cac8I 604 GCATGC GC[ACGT]^[ACGT]GC DsaI 61 CCATGG C^C[GA][CT]GG Eco81I 578 CCTCAGG CC^T[ACGT]AGG EcoNI 146 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 362 GAATTC G^AATTC EcoT14I 61 CCATGG C^C[AT][AT]GG FauI 48 GCGGG CCCGC(4/6) FbaI 416 TGATCA T^GATCA FokI 125 GGATG GGATG(9/13) MaeII 107 ACGT A^CGT MflI 338 GGATCC [GA]^GATC[CT] NcoI 61 CCATGG C^CATGG PflMI 195 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 305 AGGACCC [GA]G^G[AT]CC[CT] PstI 472 CTGCAG CTGCA^G RsaI 535 GTAC GT^AC SexAI 350 ACCTGGT A^CC[AT]GGT SfaNI 126 GATGC GCATC(5/9) SphI 604 GCATGC GCATG^C StyI 61 CCATGG C^C[AT][AT]GG Tsp45I 452 GTGAC ^GT[GC]AC Tsp509I 363 AATT ^AATT Van91I 195 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 338 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwI 339 GATCC GGATC(4/5) 338 GGATC DpnII 339 GATC ^GATC 417 GATC DraII 92 AGGGCCT [GA]G^G[ACGT]CC[CT] 305 AGGACCC EcoO109I 92 AGGGCCT [GA]G^G[ACGT]CC[CT] 305 AGGACCC MboI 339 GATC ^GATC 417 GATC MslI 12 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 445 CACAATAGTG NdeII 339 GATC ^GATC 417 GATC NspI 466 GCATGT [GA]CATG^[CT] 604 GCATGC Sau3AI 339 GATC ^GATC 417 GATC SmlI 333 CTCAAG C^T[CT][GA]AG 630 CTTAAG TspRI 139 CAGTG CA[GC]TG[ACGT][ACGT]^ 160 CAGTG XcmI 61 CCATGGCACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 542 CCATTTACAAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AvaII BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI EcoRII HaeIII HphI MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfcI Tru9I