Restriction map for
pFN21AE1699
(Circular) (Six-base) MAPSORT of: pFN21AE1699.seq Check: 1220 from: 1 to: 5401
pFN21AE1699 5401 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2393 3464 4848 5116 1632
Size: 506 255 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1071 506 268 255
AflII C'TTAA_G
Cuts at: 820 1017 2635 820
Size: 197 1618 3586
Fragments arranged by size:
3586 1618 197
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 2021 2454 1166
Size: 855 433 4113
Fragments arranged by size:
4113 855 433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5401
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 2303
Size: 32 5369
AlwNI CAG_nnn'CTG
Cuts at: 3144 4716 3144
Size: 1572 3829
ApaI G_GGCC'C
Cuts at: 2499 2499
Size: 5401
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2367 2710 2936 3088 5197 1493
Size: 874 343 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 1870
Size: 73 783 4545
Fragments arranged by size:
4545 783 73
BamHI G'GATC_C
Cuts at: 2343 2731 3098 2343
Size: 388 367 4646
Fragments arranged by size:
4646 388 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 4177 618
Size: 325 217 627 935 1455 1842
Fragments arranged by size:
1842 1455 935 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2499 2720 1391
Size: 1108 221 4072
Fragments arranged by size:
4072 1108 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3592 3626 1903
Size: 34 1655 34 3678
Fragments arranged by size:
3678 1655 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 2421 1548
Size: 873 4528
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2818 2818
Size: 5401
Bme1580I G_kGCm'C
Cuts at: 1790 2294 2499 3462 4618 1790
Size: 504 205 963 1156 2573
Fragments arranged by size:
2573 1156 963 504 205
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2197 3132 4090
Size: 416 53 83 399 24 780 935 958
Cuts at: 4090 442
Size: 1753
Fragments arranged by size:
1753 958 935 780 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2248 4061 1239
Size: 1009 1813 2579
Fragments arranged by size:
2579 1813 1009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 3526 4391 4689 2323
Size: 1203 865 298 3035
Fragments arranged by size:
3035 1203 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 4064 882
Size: 617 581 1984 2219
Fragments arranged by size:
2219 1984 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 3192 493
Size: 711 1988 2702
Fragments arranged by size:
2702 1988 711
BsaBI GATnn'nnATC
Cuts at: 5395 5395
Size: 5401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3208 3590 4225 275
Size: 53 83 186 936 1675 382 635 1451
Fragments arranged by size:
1675 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2580 2610 1178
Size: 30 165 30 1177 30 3969
Fragments arranged by size:
3969 1177 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2249 2423 2576 1186
Size: 975 88 174 153 4011
Fragments arranged by size:
4011 975 174 153 88
BseYI C'CCAG_C
Cuts at: 2009 2492 4604 5100 2009
Size: 483 2112 496 2310
Fragments arranged by size:
2310 2112 496 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4828
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3612 4640 1540
Size: 275 186 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2171 3462 3547 4618 2171
Size: 1291 85 1071 2954
Fragments arranged by size:
2954 1291 1071 85
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2294 2499 2720 3462 3547 4618
Size: 399 381 123 205 221 742 85 1071
Cuts at: 4618 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 381 221 205 123
85
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 844
Size: 1912 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4045 4232 1700
Size: 110 2235 187 2869
Fragments arranged by size:
2869 2235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2646 794
Size: 1852 3549
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2343 2731 3098 3483 3500 4952 5038 5050
Size: 371 388 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 388 385 371 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2584 2584
Size: 5401
BtgI C'CryG_G
Cuts at: 513 1065 2004 2066 513
Size: 552 939 62 3848
Fragments arranged by size:
3848 939 552 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 2917 3711 3731 2150
Size: 767 794 20 3820
Fragments arranged by size:
3820 794 767 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2848 3737 5366
Size: 54 1425 50 275 18 1018 889 1629
Cuts at: 5366 8
Size: 43
Fragments arranged by size:
1629 1425 1018 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3331 1353
Size: 1978 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2248 2393 3464 4061 4848 5116
Size: 393 506 110 145 1071 597 787 268
Cuts at: 5116 1239
Size: 1524
Fragments arranged by size:
1524 1071 787 597 506 393 268 145
110
EcoNI CCTnn'n_nnAGG
Cuts at: 2155 2155
Size: 5401
EcoO109I rG'GnC_Cy
Cuts at: 2098 2311 2098
Size: 213 5188
EcoRI G'AATT_C
Cuts at: 2367 2710 3088 2367
Size: 343 378 4680
Fragments arranged by size:
4680 378 343
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 4548 1678
Size: 2870 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2580 2612 4042
Size: 32 135 32 653 32 323 32 1430
Cuts at: 4042 4074 4116 4148 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1430 653 323 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4515 4699 1305
Size: 3210 184 2007
Fragments arranged by size:
3210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3579
Fragments arranged by size:
3579 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2454 3359 3718 3877 518
Size: 648 855 433 905 359 159 2042
Fragments arranged by size:
2042 905 855 648 433 359 159
MspA1I CmG'CkG
Cuts at: 1606 2817 3494 4642 4887 1606
Size: 1211 677 1148 245 2120
Fragments arranged by size:
2120 1211 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2066 513
Size: 552 939 62 3848
Fragments arranged by size:
3848 939 552 62
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2475 2613 2759 4304 1132
Size: 97 1246 138 146 1545 2229
Fragments arranged by size:
2229 1545 1246 146 138 97
PasI CC'CwG_GG
Cuts at: 1348 2577 1348
Size: 1229 4172
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 1108
Size: 1098 4303
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2311 2311
Size: 5401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PspOMI G'GGCC_C
Cuts at: 2495 2495
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2481 2753 830
Size: 1651 272 3478
Fragments arranged by size:
3478 1651 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SexAI A'CCwGG_T
Cuts at: 2355 2355
Size: 5401
SfcI C'TryA_G
Cuts at: 826 1045 2383 2477 2626 2697 2749 3884 4565
Size: 219 1338 94 149 71 52 1135 681
Cuts at: 4565 4756 826
Size: 191 1471
Fragments arranged by size:
1471 1338 1135 681 219 191 149 94
71 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 2635 3505 4406 4668 820
Size: 197 926 395 297 870 901 262 1553
Fragments arranged by size:
1553 926 901 870 395 297 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2613 2759 1132
Size: 1481 146 3774
Fragments arranged by size:
3774 1481 146
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2004 2066 2840 513
Size: 552 939 62 774 3074
Fragments arranged by size:
3074 939 774 552 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3428 3445 3598 3783 4228 325
Size: 1255 1848 17 153 185 445 1498
Fragments arranged by size:
1848 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2539 3647 5359
Size: 274 80 33 51 1443 562 1108 1712
Cuts at: 5359 96
Size: 138
Fragments arranged by size:
1712 1443 1108 562 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2073 2554 2073
Size: 481 4920
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI