Restriction map for
pFN21AE6789
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AviII BamHI BbrPI
BclI BfrI BglII BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FbaI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 725 GGTACC G^GTACC
AccII 1387 CGCG CG^CG
AlwNI 172 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 725 GGTACC G^GTACC
AspI 957 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1318 CCCGAG C^[CT]CG[GA]G
AvrII 849 CCTAGG C^CTAGG
BalI 1139 TGGCCA TGG^CCA
BbeI 1486 GGCGCC GGCGC^C
BcgI 161 CGAAGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1271 GTATCC GTATCC(6/5)
BfaI 850 CTAG C^TAG
BlnI 849 CCTAGG C^CTAGG
BsaAI 1600 TACGTG [CT]AC^GT[GA]
BsgI 742 GTGCAG GTGCAG(16/14)
BsmBI 774 CGTCTC CGTCTC(1/5)
BsoBI 1318 CCCGAG C^[CT]CG[GA]G
BspMI 512 ACCTGC ACCTGC(4/8)
BsrBI 1216 GAGCGG CCGCTC(-3/-3)
BssSI 559 CACGAG CACGAG(-5/-1)
BstEII 1047 GGTCACC G^GT[ACGT]ACC
BstPI 1047 GGTCACC G^GT[ACGT]ACC
BstUI 1387 CGCG CG^CG
BstXI 1661 CCACCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 196 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1047 GGTCACC G^GT[ACGT]ACC
EcoT22I 1030 ATGCAT ATGCA^T
FseI 1399 GGCCGGCC GGCCGG^CC
KasI 1486 GGCGCC G^GCGCC
KpnI 725 GGTACC GGTAC^C
MaeI 850 CTAG C^TAG
MluNI 1139 TGGCCA TGG^CCA
MscI 1139 TGGCCA TGG^CCA
MseI 1535 TTAA T^TAA
MvnI 1387 CGCG CG^CG
NarI 1486 GGCGCC GG^CGCC
NdeI 1097 CATATG CA^TATG
NsiI 1030 ATGCAT ATGCA^T
PflFI 957 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1087 GAGCTC GAGCT^C
ScaI 383 AGTACT AGT^ACT
SfoI 1486 GGCGCC GGC^GCC
TfiI 1334 GAATC G^A[AT]TC
Tru9I 1535 TTAA T^TAA
Tth111I 957 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1379 CCATACGGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 1473 GGCGTC G[GA]^CG[CT]C
1486 GGCGCC
Alw44I 204 GTGCAC G^TGCAC
599 GTGCAC
ApaI 753 GGGCCC GGGCC^C
1037 GGGCCC
ApaLI 204 GTGCAC G^TGCAC
599 GTGCAC
BbsI 660 GAAGAC GAAGAC(2/6)
948 GAAGAC
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
268 GCTGAGG
BglI 556 GCCCACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1489 GCCTCCGAGGC
BlpI 624 GCTCAGC GC^T[ACGT]AGC
1258 GCTGAGC
Bpu1102I 624 GCTCAGC GC^T[ACGT]AGC
1258 GCTGAGC
BpuAI 660 GAAGAC GAAGAC(2/6)
948 GAAGAC
BsaHI 1473 GGCGTC G[GA]^CG[CT]C
1486 GGCGCC
BsmAI 775 GTCTC GTCTC(1/5)
1510 GTCTC
BspHI 504 TCATGA T^CATGA
809 TCATGA
BsrDI 782 GCAATG GCAATG(2/0)
1007 GCAATG
CelII 624 GCTCAGC GC^T[ACGT]AGC
1258 GCTGAGC
EaeI 1139 TGGCCA [CT]^GGCC[GA]
1402 CGGCCA
EcoNI 177 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
849 CCTAGGCGAGG
HaeII 329 GGCGCT [GA]GCGC^[CT]
1486 GGCGCC
HgaI 1134 GACGC GACGC(5/10)
1474 GCGTC
Hin1I 1473 GGCGTC G[GA]^CG[CT]C
1486 GGCGCC
NaeI 671 GCCGGC GCC^GGC
1400 GCCGGC
NcoI 288 CCATGG C^CATGG
1525 CCATGG
NgoMIV 671 GCCGGC G^CCGGC
1400 GCCGGC
PleI 893 GACTC GAGTC(4/5)
842 GAGTC
PpuMI 1289 AGGTCCC [GA]G^G[AT]CC[CT]
1707 GGGACCC
PvuII 655 CAGCTG CAG^CTG
1042 CAGCTG
RcaI 504 TCATGA T^CATGA
809 TCATGA
SexAI 721 ACCTGGT A^CC[AT]GGT
1667 ACCTGGT
SphI 81 GCATGC GCATG^C
1028 GCATGC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII