(Linear) (Six-base) MAPSORT of: KIEE6789.seq Check: 8068 from: 1 to: 1740 KIEE6789.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. Acc65I G'GTAC_C Cuts at: 0 725 1740 Size: 725 1015 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 679 1564 1740 Size: 679 885 176 Fragments arranged by size: 885 679 176 AleI CACnn'nnGTG Cuts at: 0 1351 1600 1740 Size: 1351 249 140 Fragments arranged by size: 1351 249 140 AlwNI CAG_nnn'CTG Cuts at: 0 177 1740 Size: 177 1563 ApaI G_GGCC'C Cuts at: 0 757 1041 1740 Size: 757 284 699 Fragments arranged by size: 757 699 284 ApaLI G'TGCA_C Cuts at: 0 204 599 1740 Size: 204 395 1141 Fragments arranged by size: 1141 395 204 AvaI C'yCGr_G Cuts at: 0 1318 1740 Size: 1318 422 AvrII C'CTAG_G Cuts at: 0 849 1740 Size: 849 891 BanI G'GyrC_C Cuts at: 0 725 1466 1486 1740 Size: 725 741 20 254 Fragments arranged by size: 741 725 254 20 BanII G_rGCy'C Cuts at: 0 325 757 1041 1091 1740 Size: 325 432 284 50 649 Fragments arranged by size: 649 432 325 284 50 BbeI G_GCGC'C Cuts at: 0 1490 1740 Size: 1490 250 BbsI GAAGACnn'nnnn_ Cuts at: 0 667 955 1740 Size: 667 288 785 Fragments arranged by size: 785 667 288 BbvCI CC'TCA_GC Cuts at: 0 112 269 1740 Size: 112 157 1471 Fragments arranged by size: 1471 157 112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 150 184 1740 Size: 150 34 1556 Fragments arranged by size: 1556 150 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1282 1740 Size: 1282 458 BfrBI ATG'CAT Cuts at: 0 1032 1740 Size: 1032 708 BglI GCCn_nnn'nGGC Cuts at: 0 562 1495 1740 Size: 562 933 245 Fragments arranged by size: 933 562 245 BlpI GC'TnA_GC Cuts at: 0 625 1259 1740 Size: 625 634 481 Fragments arranged by size: 634 625 481 Bme1580I G_kGCm'C Cuts at: 0 208 603 654 757 1041 1740 Size: 208 395 51 103 284 699 Fragments arranged by size: 699 395 284 208 103 51 BmgBI CAC'GTC Cuts at: 0 453 498 1740 Size: 453 45 1242 Fragments arranged by size: 1242 453 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 988 1147 1740 Size: 115 873 159 593 Fragments arranged by size: 873 593 159 115 Bpu10I CC'TnA_GC Cuts at: 0 112 269 1214 1740 Size: 112 157 945 526 Fragments arranged by size: 945 526 157 112 BsaAI yAC'GTr Cuts at: 0 1602 1740 Size: 1602 138 BsaHI Gr'CG_yC Cuts at: 0 1474 1487 1740 Size: 1474 13 253 Fragments arranged by size: 1474 253 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 307 337 1450 1480 1546 1576 1740 Size: 307 30 1113 30 66 30 164 Fragments arranged by size: 1113 307 164 66 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 115 286 871 1740 Size: 112 3 171 585 869 Fragments arranged by size: 869 585 171 112 3 BseYI C'CCAG_C Cuts at: 0 138 587 1040 1740 Size: 138 449 453 700 Fragments arranged by size: 700 453 449 138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 763 1740 Size: 763 977 BsiHKAI G_wGCw'C Cuts at: 0 208 603 1091 1740 Size: 208 395 488 649 Fragments arranged by size: 649 488 395 208 BsmBI CGTCTCn'nnnn_ Cuts at: 0 780 1740 Size: 780 960 Bsp1286I G_dGCh'C Cuts at: 0 208 325 603 654 757 1041 1091 1740 Size: 208 117 278 51 103 284 50 649 Fragments arranged by size: 649 284 278 208 117 103 51 50 BspHI T'CATG_A Cuts at: 0 504 809 1740 Size: 504 305 931 Fragments arranged by size: 931 504 305 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 521 1740 Size: 521 1219 BsrBI CCG'CTC Cuts at: 0 1218 1740 Size: 1218 522 BsrDI GCAATG_nn' Cuts at: 0 789 1014 1740 Size: 789 225 726 Fragments arranged by size: 789 726 225 BsrFI r'CCGG_y Cuts at: 0 435 671 1400 1740 Size: 435 236 729 340 Fragments arranged by size: 729 435 340 236 BssSI C'ACGA_G Cuts at: 0 559 1740 Size: 559 1181 BstEII G'GTnAC_C Cuts at: 0 1047 1740 Size: 1047 693 BstXI CCAn_nnnn'nTGG Cuts at: 0 1668 1740 Size: 1668 72 BstYI r'GATC_y Cuts at: 0 173 788 1520 1697 1740 Size: 173 615 732 177 43 Fragments arranged by size: 732 615 177 173 43 BtgI C'CryG_G Cuts at: 0 288 362 1068 1294 1411 1525 1740 Size: 288 74 706 226 117 114 215 Fragments arranged by size: 706 288 226 215 117 114 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 447 1740 Size: 447 1293 BtsI GCAGTG_nn' Cuts at: 0 166 1740 Size: 166 1574 DraIII CAC_nnn'GTG Cuts at: 0 201 1740 Size: 201 1539 EaeI y'GGCC_r Cuts at: 0 1139 1402 1740 Size: 1139 263 338 Fragments arranged by size: 1139 338 263 EarI CTCTTCn'nnn_ Cuts at: 0 529 577 1063 1096 1333 1740 Size: 529 48 486 33 237 407 Fragments arranged by size: 529 486 407 237 48 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1194 1740 Size: 1194 546 EcoICRI GAG'CTC Cuts at: 0 1089 1740 Size: 1089 651 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 679 988 1147 1564 1740 Size: 115 564 309 159 417 176 Fragments arranged by size: 564 417 309 176 159 115 EcoNI CCTnn'n_nnAGG Cuts at: 0 181 853 1740 Size: 181 672 887 Fragments arranged by size: 887 672 181 EcoO109I rG'GnC_Cy Cuts at: 0 753 1037 1290 1708 1740 Size: 753 284 253 418 32 Fragments arranged by size: 753 418 284 253 32 FseI GG_CCGG'CC Cuts at: 0 1404 1740 Size: 1404 336 HaeII r_GCGC'y Cuts at: 0 333 1490 1740 Size: 333 1157 250 Fragments arranged by size: 1157 333 250 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1348 1380 1740 Size: 1348 32 360 Fragments arranged by size: 1348 360 32 KasI G'GCGC_C Cuts at: 0 1486 1740 Size: 1486 254 KpnI G_GTAC'C Cuts at: 0 729 1740 Size: 729 1011 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 284 1740 Size: 284 1456 MscI TGG'CCA Cuts at: 0 1141 1740 Size: 1141 599 MslI CAynn'nnrTG Cuts at: 0 196 1351 1600 1740 Size: 196 1155 249 140 Fragments arranged by size: 1155 249 196 140 MspA1I CmG'CkG Cuts at: 0 49 657 1044 1682 1740 Size: 49 608 387 638 58 Fragments arranged by size: 638 608 387 58 49 NaeI GCC'GGC Cuts at: 0 673 1402 1740 Size: 673 729 338 Fragments arranged by size: 729 673 338 NarI GG'CG_CC Cuts at: 0 1487 1740 Size: 1487 253 NcoI C'CATG_G Cuts at: 0 288 1525 1740 Size: 288 1237 215 Fragments arranged by size: 1237 288 215 NdeI CA'TA_TG Cuts at: 0 1098 1740 Size: 1098 642 NgoMIV G'CCGG_C Cuts at: 0 671 1400 1740 Size: 671 729 340 Fragments arranged by size: 729 671 340 NsiI A_TGCA'T Cuts at: 0 1034 1740 Size: 1034 706 NspI r_CATG'y Cuts at: 0 67 85 1032 1740 Size: 67 18 947 708 Fragments arranged by size: 947 708 67 18 PasI CC'CwG_GG Cuts at: 0 666 750 1223 1740 Size: 666 84 473 517 Fragments arranged by size: 666 517 473 84 PpuMI rG'GwC_Cy Cuts at: 0 1290 1708 1740 Size: 1290 418 32 Fragments arranged by size: 1290 418 32 PspOMI G'GGCC_C Cuts at: 0 753 1037 1740 Size: 753 284 703 Fragments arranged by size: 753 703 284 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1522 1554 1740 Size: 1522 32 186 Fragments arranged by size: 1522 186 32 PstI C_TGCA'G Cuts at: 0 173 1580 1625 1740 Size: 173 1407 45 115 Fragments arranged by size: 1407 173 115 45 PvuII CAG'CTG Cuts at: 0 657 1044 1740 Size: 657 387 696 Fragments arranged by size: 696 657 387 SacI G_AGCT'C Cuts at: 0 1091 1740 Size: 1091 649 SanDI GG'GwC_CC Cuts at: 0 1708 1740 Size: 1708 32 SapI GCTCTTCn'nnn_ Cuts at: 0 577 1096 1333 1740 Size: 577 519 237 407 Fragments arranged by size: 577 519 407 237 ScaI AGT'ACT Cuts at: 0 385 1740 Size: 385 1355 SexAI A'CCwGG_T Cuts at: 0 721 1667 1740 Size: 721 946 73 Fragments arranged by size: 946 721 73 SfcI C'TryA_G Cuts at: 0 169 1576 1621 1740 Size: 169 1407 45 119 Fragments arranged by size: 1407 169 119 45 SfoI GGC'GCC Cuts at: 0 1488 1740 Size: 1488 252 SphI G_CATG'C Cuts at: 0 85 1032 1740 Size: 85 947 708 Fragments arranged by size: 947 708 85 StyI C'CwwG_G Cuts at: 0 288 849 1349 1525 1740 Size: 288 561 500 176 215 Fragments arranged by size: 561 500 288 215 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1258 1740 Size: 1258 482 TatI w'GTAC_w Cuts at: 0 383 860 1740 Size: 383 477 880 Fragments arranged by size: 880 477 383 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 158 190 1450 1482 1740 Size: 158 32 1260 32 258 Fragments arranged by size: 1260 258 158 32 32 Tth111I GACn'n_nGTC Cuts at: 0 960 1740 Size: 960 780 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1386 1740 Size: 1386 354 Enzymes that do cut: Acc65I AcuI AleI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PpuMI PspOMI PsrI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SphI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI BaeI BamHI BclI BglII BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI MfeI MluI NheI NotI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI ZraI