(Circular) (Six-base) MAPSORT of: pFN21AE4169.seq Check: 4310 from: 1 to: 6568 pFN21AE4169 6568 bp With 182 enzymes: SgfI * March 28, 2012 13:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3806 3806 Size: 6568 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3396 3911 1937 Size: 1459 515 4594 Fragments arranged by size: 4594 1459 515 Acc65I G'GTAC_C Cuts at: 3674 3889 3674 Size: 215 6353 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4631 6015 6283 1632 Size: 2999 1384 268 1917 Fragments arranged by size: 2999 1917 1384 268 AfeI AGC'GCT Cuts at: 3284 3284 Size: 6568 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 5467 6444 1203 Size: 4264 977 1327 Fragments arranged by size: 4264 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 3316 5297 3316 Size: 1981 4587 AleI CACnn'nnGTG Cuts at: 1166 2489 1166 Size: 1323 5245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2966 1184 Size: 1782 4786 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2570 2602 3714 3746 2570 Size: 32 1112 32 5392 Fragments arranged by size: 5392 1112 32 32 AlwNI CAG_nnn'CTG Cuts at: 2260 4311 5883 2260 Size: 2051 1572 2945 Fragments arranged by size: 2945 2051 1572 ApaLI G'TGCA_C Cuts at: 4625 5781 4625 Size: 1156 5412 ApoI r'AATT_y Cuts at: 1493 3877 4103 4255 6364 1493 Size: 2384 226 152 2109 1697 Fragments arranged by size: 2384 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 2747 3893 1870 Size: 73 804 1146 4545 Fragments arranged by size: 4545 1146 804 73 BamHI G'GATC_C Cuts at: 2916 3898 4265 2916 Size: 982 367 5219 Fragments arranged by size: 5219 982 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3484 3616 3674 3818 3889 Size: 325 217 627 1697 132 58 144 71 Cuts at: 3889 5344 618 Size: 1455 1842 Fragments arranged by size: 1842 1697 1455 627 325 217 144 132 71 58 BanII G_rGCy'C Cuts at: 1391 2073 2535 3744 3887 1391 Size: 682 462 1209 143 4072 Fragments arranged by size: 4072 1209 682 462 143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2404 2656 3079 3123 928 Size: 971 505 252 423 44 4373 Fragments arranged by size: 4373 971 505 423 252 44 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2083 2117 4759 4793 1903 Size: 34 146 34 2642 34 3678 Fragments arranged by size: 3678 2642 146 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2915 4460 5670 2915 Size: 1545 1210 3813 Fragments arranged by size: 3813 1545 1210 BclI T'GATC_A Cuts at: 1548 2970 1548 Size: 1422 5146 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2849 3219 5179 Size: 107 122 71 631 605 1177 370 1960 Cuts at: 5179 136 Size: 1525 Fragments arranged by size: 1960 1525 1177 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 6563 6563 Size: 6568 BlpI GC'TnA_GC Cuts at: 2637 3985 2637 Size: 1348 5220 Bme1580I G_kGCm'C Cuts at: 1790 2743 2864 3619 4629 5785 1790 Size: 953 121 755 1010 1156 2573 Fragments arranged by size: 2573 1156 1010 953 755 121 BmgBI CAC'GTC Cuts at: 1318 2245 3058 1318 Size: 927 813 4828 Fragments arranged by size: 4828 927 813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4299 5257 442 Size: 416 53 83 399 24 2882 958 1753 Fragments arranged by size: 2882 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3251 3664 3251 Size: 413 6155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2533 3117 3728 3741 3775 5228 1239 Size: 1007 287 584 611 13 34 1453 2579 Fragments arranged by size: 2579 1453 1007 611 584 287 34 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4693 5558 5856 4693 Size: 865 298 5405 Fragments arranged by size: 5405 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5231 882 Size: 617 3732 2219 Fragments arranged by size: 3732 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3250 3355 3400 4359 493 Size: 711 2046 105 45 959 2702 Fragments arranged by size: 2702 2046 959 711 105 45 BsaBI GATnn'nnATC Cuts at: 6562 6562 Size: 6568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2622 4375 4757 5392 Size: 53 83 186 936 1089 1753 382 635 Cuts at: 5392 275 Size: 1451 Fragments arranged by size: 1753 1451 1089 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5001 5399 5673 5820 6231 5001 Size: 398 274 147 411 5338 Fragments arranged by size: 5338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 2219 2249 2570 Size: 30 165 30 621 30 165 30 321 Cuts at: 2570 2600 3701 3714 3731 3744 1178 Size: 30 1101 13 17 13 4002 Fragments arranged by size: 4002 1101 621 321 165 165 30 30 30 30 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2243 2591 2897 2906 2993 3782 3785 Size: 859 198 348 306 9 87 789 3 Cuts at: 3785 1186 Size: 3969 Fragments arranged by size: 3969 859 789 348 306 198 87 9 3 BseYI C'CCAG_C Cuts at: 2011 2742 3181 3551 5771 6267 2011 Size: 731 439 370 2220 496 2312 Fragments arranged by size: 2312 2220 731 496 439 370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2246 2327 2786 1163 Size: 573 468 42 81 459 4945 Fragments arranged by size: 4945 573 468 459 81 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2526 4018 4779 5807 1540 Size: 275 186 525 1492 761 1028 2301 Fragments arranged by size: 2301 1492 1028 761 525 275 186 BsiHKAI G_wGCw'C Cuts at: 2535 3378 3744 4629 4714 5785 2535 Size: 843 366 885 85 1071 3318 Fragments arranged by size: 3318 1071 885 843 366 85 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 BsmBI CGTCTCn'nnnn_ Cuts at: 2423 2450 3704 2423 Size: 27 1254 5287 Fragments arranged by size: 5287 1254 27 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2535 2743 2864 3378 3619 3744 Size: 399 283 462 208 121 514 241 125 Cuts at: 3744 3887 4629 4714 5785 1391 Size: 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 462 399 283 241 208 143 125 121 85 BspEI T'CCGG_A Cuts at: 6231 6231 Size: 6568 BspHI T'CATG_A Cuts at: 2967 4457 6187 2967 Size: 1490 1730 3348 Fragments arranged by size: 3348 1730 1490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3806 3923 844 Size: 2962 117 3489 Fragments arranged by size: 3489 2962 117 BsrBI CCG'CTC Cuts at: 1394 1823 3504 4455 1394 Size: 429 1681 951 3507 Fragments arranged by size: 3507 1681 951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5063 5237 65 Size: 1183 3815 174 1396 Fragments arranged by size: 3815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5212 5399 1700 Size: 110 3402 187 2869 Fragments arranged by size: 3402 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2271 3591 96 Size: 2175 1320 3073 Fragments arranged by size: 3073 2175 1320 BssHII G'CGCG_C Cuts at: 1925 2183 2206 2439 2495 2647 2649 2695 1925 Size: 258 23 233 56 152 2 46 5798 Fragments arranged by size: 5798 258 233 152 56 46 23 2 BssSI C'ACGA_G Cuts at: 2732 2786 4628 5640 2732 Size: 54 1842 1012 3660 Fragments arranged by size: 3660 1842 1012 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 3818 794 Size: 3024 3544 BstBI TT'CG_AA Cuts at: 5372 5372 Size: 6568 BstEII G'GTnAC_C Cuts at: 1184 3067 3556 1184 Size: 1883 489 4196 Fragments arranged by size: 4196 1883 489 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2404 3250 2011 Size: 393 846 5329 Fragments arranged by size: 5329 846 393 BstYI r'GATC_y Cuts at: 1972 2340 2916 2956 3898 4265 4650 4667 6119 Size: 368 576 40 942 367 385 17 1452 Cuts at: 6119 6205 6217 6563 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 942 576 385 368 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2089 2322 2451 513 Size: 552 939 85 233 129 4630 Fragments arranged by size: 4630 939 552 233 129 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2129 2521 487 Size: 683 56 903 392 4534 Fragments arranged by size: 4534 903 683 392 56 BtsI GCAGTG_nn' Cuts at: 2589 3424 4084 4878 4898 2589 Size: 835 660 794 20 4259 Fragments arranged by size: 4259 835 794 660 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DraIII CAC_nnn'GTG Cuts at: 3373 3373 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2406 2523 Size: 54 1425 50 275 18 327 249 117 Cuts at: 2523 3364 3532 4015 4904 6533 8 Size: 841 168 483 889 1629 43 Fragments arranged by size: 1629 1425 889 841 483 327 275 249 168 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2523 4015 1537 Size: 275 711 1492 4090 Fragments arranged by size: 4090 1492 711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3110 4498 1353 Size: 1757 1388 3423 Fragments arranged by size: 3423 1757 1388 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 5155 5529 5675 387 Size: 1064 138 453 3113 374 146 1280 Fragments arranged by size: 3113 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 2533 3742 2533 Size: 1209 5359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2533 3117 3728 3741 3775 4631 Size: 393 614 287 584 611 13 34 856 Cuts at: 4631 5228 6015 6283 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 856 787 614 611 597 584 393 287 268 34 13 EcoNI CCTnn'n_nnAGG Cuts at: 2228 2228 Size: 6568 EcoO109I rG'GnC_Cy Cuts at: 3043 3130 3043 Size: 87 6481 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 FspI TGC'GCA Cuts at: 2759 5074 2759 Size: 2315 4253 HaeII r_GCGC'y Cuts at: 1678 2012 2386 2644 2725 2988 3286 5715 1678 Size: 334 374 258 81 263 298 2429 2531 Fragments arranged by size: 2531 2429 374 334 298 263 258 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 3302 3334 3701 Size: 32 135 32 647 32 1051 32 367 Cuts at: 3701 3733 5209 5241 5283 5315 1373 Size: 32 1476 32 42 32 2626 Fragments arranged by size: 2626 1476 1051 647 367 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3912 4158 669 Size: 988 281 1974 246 3079 Fragments arranged by size: 3079 1974 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6568 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KpnI G_GTAC'C Cuts at: 3678 3893 3678 Size: 215 6353 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 3060 5682 5866 1305 Size: 1116 639 2622 184 2007 Fragments arranged by size: 2622 2007 1116 639 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3366 10 Size: 54 1425 343 1534 3212 Fragments arranged by size: 3212 1534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2489 2966 3248 3325 3542 4526 Size: 648 1197 126 477 282 77 217 984 Cuts at: 4526 4885 5044 518 Size: 359 159 2042 Fragments arranged by size: 2042 1197 984 648 477 359 282 217 159 126 77 MspA1I CmG'CkG Cuts at: 1606 2038 2324 3196 3210 3346 3551 3984 4661 Size: 432 286 872 14 136 205 433 677 Cuts at: 4661 5809 6054 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 872 677 433 432 286 245 205 136 14 NcoI C'CATG_G Cuts at: 513 1065 2004 2089 513 Size: 552 939 85 4992 Fragments arranged by size: 4992 939 552 85 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 2083 2947 3926 5471 1132 Size: 97 854 864 979 1545 2229 Fragments arranged by size: 2229 1545 979 864 854 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6568 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 1108 Size: 1041 5527 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2117 2149 2570 2602 3714 3746 4637 4669 6150 Size: 32 421 32 1112 32 891 32 1481 Cuts at: 6150 6182 2117 Size: 32 2503 Fragments arranged by size: 2503 1481 1112 891 421 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3043 3130 3043 Size: 87 6481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PstI C_TGCA'G Cuts at: 830 2168 2379 2955 3156 3920 830 Size: 1338 211 576 201 764 3478 Fragments arranged by size: 3478 1338 764 576 211 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 3551 3551 Size: 6568 SacI G_AGCT'C Cuts at: 2535 3744 2535 Size: 1209 5359 SacII CC_GC'GG Cuts at: 2325 2325 Size: 6568 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SbfI CC_TGCA'GG Cuts at: 2955 3920 2955 Size: 965 5603 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SexAI A'CCwGG_T Cuts at: 3039 3081 3039 Size: 42 6526 SfcI C'TryA_G Cuts at: 826 1045 2164 2375 2951 3152 3916 5051 5732 Size: 219 1119 211 576 201 764 1135 681 Cuts at: 5732 5923 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1119 764 681 576 219 211 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4672 5573 5835 820 Size: 197 926 2729 901 262 1553 Fragments arranged by size: 2729 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3400 4359 493 Size: 2907 959 2702 Fragments arranged by size: 2907 2702 959 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 4492 5 Size: 47 4440 2081 Fragments arranged by size: 4440 2081 47 StuI AGG'CCT Cuts at: 1517 3102 3629 1517 Size: 1585 527 4456 Fragments arranged by size: 4456 1585 527 StyI C'CwwG_G Cuts at: 513 1065 2004 2089 2844 3072 4007 513 Size: 552 939 85 755 228 935 3074 Fragments arranged by size: 3074 939 935 755 552 228 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3286 3502 4595 4612 4765 4950 5395 Size: 1255 1706 216 1093 17 153 185 445 Cuts at: 5395 325 Size: 1498 Fragments arranged by size: 1706 1498 1255 1093 445 216 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 3591 4814 Size: 274 80 33 51 1443 294 1320 1223 Cuts at: 4814 6526 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1320 1223 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 3161 3193 6528 6560 2319 Size: 32 810 32 3335 32 2327 Fragments arranged by size: 3335 2327 810 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3296 3392 3715 1316 Size: 1980 96 323 4169 Fragments arranged by size: 4169 1980 323 96 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 4697 4697 Size: 6568 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI