################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MunI NciI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 792 GGTACC G^GTACC AccIII 1080 TCCGGA T^CCGGA AocI 558 CCTGAGG CC^T[ACGT]AGG Asp700I 756 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 792 GGTACC G^GTACC AvaI 230 CTCGGG C^[CT]CG[GA]G BalI 217 TGGCCA TGG^CCA BamHI 830 GGATCC G^GATCC BbrPI 1284 CACGTG CAC^GTG BbvCI 1194 GCTGAGG CCTCAGC(-5/-2) BfaI 1015 CTAG C^TAG BsaAI 1284 CACGTG [CT]AC^GT[GA] BsaWI 1080 TCCGGA [AT]^CCGG[AT] BseAI 1080 TCCGGA T^CCGGA BsmBI 735 CGTCTC CGTCTC(1/5) BsoBI 230 CTCGGG C^[CT]CG[GA]G Bsp1407I 719 TGTACA T^GTACA BspEI 1080 TCCGGA T^CCGGA BsrGI 719 TGTACA T^GTACA BsrI 618 CCAGT ACTGG(1/-1) BstXI 464 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 830 GGATCC [GA]^GATC[CT] Bsu36I 558 CCTGAGG CC^T[ACGT]AGG Eco81I 558 CCTGAGG CC^T[ACGT]AGG EcoNI 150 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 979 GATATC GAT^ATC KpnI 792 GGTACC GGTAC^C MaeI 1015 CTAG C^TAG MaeII 1285 ACGT A^CGT MflI 830 GGATCC [GA]^GATC[CT] MluNI 217 TGGCCA TGG^CCA MroI 1080 TCCGGA T^CCGGA MscI 217 TGGCCA TGG^CCA MseI 1091 TTAA T^TAA NaeI 72 GCCGGC GCC^GGC NgoMIV 72 GCCGGC G^CCGGC NheI 1014 GCTAGC G^CTAGC NspI 533 ACATGC [GA]CATG^[CT] PflMI 809 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 610 GAGTC GAGTC(4/5) PmaCI 1284 CACGTG CAC^GTG PmlI 1284 CACGTG CAC^GTG SacI 1117 GAGCTC GAGCT^C SapI 273 GCTCTTC GCTCTTC(1/4) SbfI 1221 CCTGCAGG CCTGCA^GG ScaI 758 AGTACT AGT^ACT SfaNI 941 GCATC GCATC(5/9) SmlI 1008 CTCAAG C^T[CT][GA]AG Sse8387I 1221 CCTGCAGG CCTGCA^GG SspBI 719 TGTACA T^GTACA SspI 668 AATATT AAT^ATT TfiI 950 GAATC G^A[AT]TC Tru9I 1091 TTAA T^TAA Tsp509I 1087 AATT ^AATT Van91I 809 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 830 GGATCC [GA]^GATC[CT] XmnI 756 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 518 CGCG CG^CG 1315 CGCG AlwNI 145 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 961 CAGAAACTG ApaI 62 GGGCCC GGGCC^C 894 GGGCCC AspHI 621 GTGCTC G[AT]GC[AT]^C 1117 GAGCTC BbeI 49 GGCGCC GGCGC^C 1279 GGCGCC BbsI 426 GAAGAC GAAGAC(2/6) 574 GTCTTC BpuAI 426 GAAGAC GAAGAC(2/6) 574 GTCTTC BsiHKAI 621 GTGCTC G[AT]GC[AT]^C 1117 GAGCTC BsrBI 1335 CCGCTC CCGCTC(-3/-3) 751 GAGCGG BsrDI 357 CATTGC GCAATG(2/0) 296 GCAATG BstUI 518 CGCG CG^CG 1315 CGCG DsaI 653 CCATGG C^C[GA][CT]GG 809 CCATGG HinfI 610 GAGTC G^A[ACGT]TC 950 GAATC KasI 49 GGCGCC G^GCGCC 1279 GGCGCC MaeIII 110 GTGAC ^GT[ACGT]AC 992 GTGAC MvnI 518 CGCG CG^CG 1315 CGCG NarI 49 GGCGCC GG^CGCC 1279 GGCGCC NcoI 653 CCATGG C^CATGG 809 CCATGG PshAI 994 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1311 GACCCGCGTC PvuII 163 CAGCTG CAG^CTG 309 CAGCTG SfoI 49 GGCGCC GGC^GCC 1279 GGCGCC TaqI 128 TCGA T^CGA 599 TCGA Tsp45I 110 GTGAC ^GT[GC]AC 992 GTGAC TthHB8I 128 TCGA T^CGA 599 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BanI BanII BbvI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI