(Linear) (Six-base) MAPSORT of: KIEE1921.seq Check: 1836 from: 1 to: 1371 KIEE1921.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 220 1229 1371 Size: 220 1009 142 Fragments arranged by size: 1009 220 142 Acc65I G'GTAC_C Cuts at: 0 792 1371 Size: 792 579 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 943 1118 1371 Size: 943 175 253 Fragments arranged by size: 943 253 175 AleI CACnn'nnGTG Cuts at: 0 811 879 1371 Size: 811 68 492 Fragments arranged by size: 811 492 68 AlwNI CAG_nnn'CTG Cuts at: 0 150 966 1371 Size: 150 816 405 Fragments arranged by size: 816 405 150 ApaI G_GGCC'C Cuts at: 0 66 898 1371 Size: 66 832 473 Fragments arranged by size: 832 473 66 AvaI C'yCGr_G Cuts at: 0 230 1371 Size: 230 1141 BamHI G'GATC_C Cuts at: 0 830 1371 Size: 830 541 BanI G'GyrC_C Cuts at: 0 49 792 1217 1256 1279 1327 1371 Size: 49 743 425 39 23 48 44 Fragments arranged by size: 743 425 49 48 44 39 23 BanII G_rGCy'C Cuts at: 0 66 634 898 1121 1371 Size: 66 568 264 223 250 Fragments arranged by size: 568 264 250 223 66 BbeI G_GCGC'C Cuts at: 0 53 1283 1371 Size: 53 1230 88 Fragments arranged by size: 1230 88 53 BbsI GAAGACnn'nnnn_ Cuts at: 0 433 567 1371 Size: 433 134 804 Fragments arranged by size: 804 433 134 BbvCI CC'TCA_GC Cuts at: 0 1195 1371 Size: 1195 176 Bme1580I G_kGCm'C Cuts at: 0 66 898 1332 1371 Size: 66 832 434 39 Fragments arranged by size: 832 434 66 39 BmtI G_CTAG'C Cuts at: 0 1018 1371 Size: 1018 353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 187 358 1326 1371 Size: 172 15 171 968 45 Fragments arranged by size: 968 172 171 45 15 Bpu10I CC'TnA_GC Cuts at: 0 490 1195 1371 Size: 490 705 176 Fragments arranged by size: 705 490 176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 993 1371 Size: 993 378 BsaAI yAC'GTr Cuts at: 0 1286 1371 Size: 1286 85 BsaHI Gr'CG_yC Cuts at: 0 50 1229 1280 1371 Size: 50 1179 51 91 Fragments arranged by size: 1179 91 51 50 BsaWI w'CCGG_w Cuts at: 0 1080 1371 Size: 1080 291 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 607 637 1371 Size: 607 30 734 Fragments arranged by size: 734 607 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 118 169 270 861 1329 1371 Size: 118 51 101 591 468 42 Fragments arranged by size: 591 468 118 101 51 42 BseYI C'CCAG_C Cuts at: 0 161 785 796 854 1371 Size: 161 624 11 58 517 Fragments arranged by size: 624 517 161 58 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 154 297 498 1204 1308 1371 Size: 139 15 143 201 706 104 63 Fragments arranged by size: 706 201 143 139 104 63 15 BsiHKAI G_wGCw'C Cuts at: 0 625 1121 1371 Size: 625 496 250 Fragments arranged by size: 625 496 250 BsmBI CGTCTCn'nnnn_ Cuts at: 0 741 1371 Size: 741 630 Bsp1286I G_dGCh'C Cuts at: 0 66 625 634 898 1121 1332 1371 Size: 66 559 9 264 223 211 39 Fragments arranged by size: 559 264 223 211 66 39 9 BspEI T'CCGG_A Cuts at: 0 1080 1371 Size: 1080 291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 220 1229 1287 1371 Size: 220 1009 58 84 Fragments arranged by size: 1009 220 84 58 BsrBI CCG'CTC Cuts at: 0 753 1337 1371 Size: 753 584 34 Fragments arranged by size: 753 584 34 BsrDI GCAATG_nn' Cuts at: 0 303 356 1371 Size: 303 53 1015 Fragments arranged by size: 1015 303 53 BsrFI r'CCGG_y Cuts at: 0 72 430 1253 1371 Size: 72 358 823 118 Fragments arranged by size: 823 358 118 72 BsrGI T'GTAC_A Cuts at: 0 719 1371 Size: 719 652 BstXI CCAn_nnnn'nTGG Cuts at: 0 471 1371 Size: 471 900 BstYI r'GATC_y Cuts at: 0 830 1371 Size: 830 541 Bsu36I CC'TnA_GG Cuts at: 0 559 1371 Size: 559 812 BtgI C'CryG_G Cuts at: 0 653 809 1371 Size: 653 156 562 Fragments arranged by size: 653 562 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 807 1371 Size: 807 564 BtsI GCAGTG_nn' Cuts at: 0 151 510 1371 Size: 151 359 861 Fragments arranged by size: 861 359 151 EaeI y'GGCC_r Cuts at: 0 81 89 217 1371 Size: 81 8 128 1154 Fragments arranged by size: 1154 128 81 8 EarI CTCTTCn'nnn_ Cuts at: 0 280 547 601 982 1371 Size: 280 267 54 381 389 Fragments arranged by size: 389 381 280 267 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1204 1215 1371 Size: 1204 11 156 Fragments arranged by size: 1204 156 11 EcoICRI GAG'CTC Cuts at: 0 1119 1371 Size: 1119 252 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 187 358 943 1118 1326 1371 Size: 172 15 171 585 175 208 45 Fragments arranged by size: 585 208 175 172 171 45 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 154 1371 Size: 154 1217 EcoO109I rG'GnC_Cy Cuts at: 0 63 708 894 895 1371 Size: 63 645 186 1 476 Fragments arranged by size: 645 476 186 63 1 EcoRV GAT'ATC Cuts at: 0 981 1371 Size: 981 390 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1128 1371 Size: 1128 243 HaeII r_GCGC'y Cuts at: 0 53 1215 1283 1371 Size: 53 1162 68 88 Fragments arranged by size: 1162 88 68 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 607 639 1371 Size: 607 32 732 Fragments arranged by size: 732 607 32 KasI G'GCGC_C Cuts at: 0 49 1279 1371 Size: 49 1230 92 Fragments arranged by size: 1230 92 49 KpnI G_GTAC'C Cuts at: 0 796 1371 Size: 796 575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 593 1371 Size: 593 778 MscI TGG'CCA Cuts at: 0 219 1371 Size: 219 1152 MslI CAynn'nnrTG Cuts at: 0 532 811 879 1371 Size: 532 279 68 492 Fragments arranged by size: 532 492 279 68 MspA1I CmG'CkG Cuts at: 0 18 29 165 311 571 605 1371 Size: 18 11 136 146 260 34 766 Fragments arranged by size: 766 260 146 136 34 18 11 NaeI GCC'GGC Cuts at: 0 74 1371 Size: 74 1297 NarI GG'CG_CC Cuts at: 0 50 1280 1371 Size: 50 1230 91 Fragments arranged by size: 1230 91 50 NcoI C'CATG_G Cuts at: 0 653 809 1371 Size: 653 156 562 Fragments arranged by size: 653 562 156 NgoMIV G'CCGG_C Cuts at: 0 72 1371 Size: 72 1299 NheI G'CTAG_C Cuts at: 0 1014 1371 Size: 1014 357 NspI r_CATG'y Cuts at: 0 537 1371 Size: 537 834 PflMI CCAn_nnn'nTGG Cuts at: 0 815 1371 Size: 815 556 PmlI CAC'GTG Cuts at: 0 1286 1371 Size: 1286 85 PshAI GACnn'nnGTC Cuts at: 0 998 1315 1371 Size: 998 317 56 Fragments arranged by size: 998 317 56 PspOMI G'GGCC_C Cuts at: 0 62 894 1371 Size: 62 832 477 Fragments arranged by size: 832 477 62 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1234 1266 1371 Size: 1234 32 105 Fragments arranged by size: 1234 105 32 PstI C_TGCA'G Cuts at: 0 101 190 1226 1371 Size: 101 89 1036 145 Fragments arranged by size: 1036 145 101 89 PvuII CAG'CTG Cuts at: 0 165 311 1371 Size: 165 146 1060 Fragments arranged by size: 1060 165 146 SacI G_AGCT'C Cuts at: 0 1121 1371 Size: 1121 250 SapI GCTCTTCn'nnn_ Cuts at: 0 280 1371 Size: 280 1091 SbfI CC_TGCA'GG Cuts at: 0 1226 1371 Size: 1226 145 ScaI AGT'ACT Cuts at: 0 760 1371 Size: 760 611 SfcI C'TryA_G Cuts at: 0 97 186 1222 1371 Size: 97 89 1036 149 Fragments arranged by size: 1036 149 97 89 SfoI GGC'GCC Cuts at: 0 51 1281 1371 Size: 51 1230 90 Fragments arranged by size: 1230 90 51 SmlI C'TyrA_G Cuts at: 0 1008 1371 Size: 1008 363 SspI AAT'ATT Cuts at: 0 670 1371 Size: 670 701 StyI C'CwwG_G Cuts at: 0 66 653 809 1371 Size: 66 587 156 562 Fragments arranged by size: 587 562 156 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 839 1371 Size: 839 532 TatI w'GTAC_w Cuts at: 0 719 758 1053 1371 Size: 719 39 295 318 Fragments arranged by size: 719 318 295 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 835 867 1371 Size: 835 32 504 Fragments arranged by size: 835 504 32 XmnI GAAnn'nnTTC Cuts at: 0 760 1371 Size: 760 611 Enzymes that do cut: AarI Acc65I AcuI AleI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PflMI PmlI PshAI PspOMI PsrI PstI PvuII SacI SapI SbfI ScaI SfcI SfoI SmlI SspI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI BsaI BsaBI BsiEI BsiWI BsmI BspHI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI PsiI PspXI PvuI RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI