################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AccII AclI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BclI BfrI BlnI BsaAI BsaBI BsaI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1163 GACGTC GACGT^C AccI 1534 GTCTAC GT^[AC][GT]AC AccIII 1602 TCCGGA T^CCGGA AcyI 1163 GACGTC G[GA]^CG[CT]C AlwI 1035 GATCC GGATC(4/5) Aor51HI 791 AGCGCT AGC^GCT Asp700I 87 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 91 GTCTTC GAAGAC(2/6) BcgI 9 GCACGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1051 GTATCC GTATCC(6/5) BglII 123 AGATCT A^GATCT BlpI 732 GCTTAGC GC^T[ACGT]AGC Bpu1102I 732 GCTTAGC GC^T[ACGT]AGC BpuAI 91 GTCTTC GAAGAC(2/6) BsaHI 1163 GACGTC G[GA]^CG[CT]C BsaWI 1602 TCCGGA [AT]^CCGG[AT] BseAI 1602 TCCGGA T^CCGGA BsmI 1201 GCATTC GAATGC(1/-1) BspEI 1602 TCCGGA T^CCGGA BspHI 1022 TCATGA T^CATGA BsrDI 882 CATTGC GCAATG(2/0) BstXI 1091 CCACAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 732 GCTTAGC GC^T[ACGT]AGC CfoI 792 GCGC GCG^C DraI 1006 TTTAAA TTT^AAA DrdI 880 GACATTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 791 AGCGCT AGC^GCT EcoRV 217 GATATC GAT^ATC FauI 283 GCGGG CCCGC(4/6) HaeII 791 AGCGCT [GA]GCGC^[CT] HgaI 847 GCGTC GACGC(5/10) HhaI 792 GCGC GCG^C Hin1I 1163 GACGTC G[GA]^CG[CT]C HinP1I 792 GCGC G^CGC HincII 973 GTTGAC GT[CT]^[GA]AC HindII 973 GTTGAC GT[CT]^[GA]AC MroI 1602 TCCGGA T^CCGGA PaeR7I 1567 CTCGAG C^TCGAG PpuMI 490 GGGTCCC [GA]G^G[AT]CC[CT] PstI 1084 CTGCAG CTGCA^G RcaI 1022 TCATGA T^CATGA ScaI 192 AGTACT AGT^ACT XhoI 1567 CTCGAG C^TCGAG XmnI 87 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AfaI 193 GTAC GT^AC 745 GTAC Alw44I 160 GTGCAC G^TGCAC 710 GTGCAC ApaLI 160 GTGCAC G^TGCAC 710 GTGCAC BalI 1250 TGGCCA TGG^CCA 1310 TGGCCA BanI 703 GGTGCC G^G[CT][GA]CC 898 GGCACC BanII 320 GAGCCC G[GA]GC[CT]^C 931 GAGCCC BfaI 268 CTAG C^TAG 1501 CTAG BglI 322 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1396 GCCAACTCGGC BseRI 724 GAGGAG GAGGAG(10/8) 1319 GAGGAG BsmAI 864 GAGAC GTCTC(1/5) 1584 GTCTC BspMI 54 GCAGGT ACCTGC(4/8) 1444 GCAGGT BstYI 123 AGATCT [GA]^GATC[CT] 1034 AGATCC EaeI 1250 TGGCCA [CT]^GGCC[GA] 1310 TGGCCA EcoT14I 612 CCTTGG C^C[AT][AT]GG 699 CCTTGG HindIII 339 AAGCTT A^AGCTT 730 AAGCTT MaeI 268 CTAG C^TAG 1501 CTAG MaeII 785 ACGT A^CGT 1164 ACGT MflI 123 AGATCT [GA]^GATC[CT] 1034 AGATCC MluNI 1250 TGGCCA TGG^CCA 1310 TGGCCA MscI 1250 TGGCCA TGG^CCA 1310 TGGCCA MseI 1007 TTAA T^TAA 1175 TTAA PflMI 130 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 207 CCAGGCACTGG PvuII 442 CAGCTG CAG^CTG 477 CAGCTG RsaI 193 GTAC GT^AC 745 GTAC SfcI 636 CTACAG C^T[GA][CT]AG 1084 CTGCAG SmaI 1518 CCCGGG CCC^GGG 1592 CCCGGG SmlI 260 CTCAAG C^T[CT][GA]AG 1567 CTCGAG StuI 554 AGGCCT AGG^CCT 1272 AGGCCT StyI 612 CCTTGG C^C[AT][AT]GG 699 CCTTGG TfiI 1260 GATTC G^A[AT]TC 1577 GAATC Tru9I 1007 TTAA T^TAA 1175 TTAA Van91I 130 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 207 CCAGGCACTGG XhoII 123 AGATCT [GA]^GATC[CT] 1034 AGATCC XmaCI 1518 CCCGGG C^CCGGG 1592 CCCGGG XmaI 1518 CCCGGG C^CCGGG 1592 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AluI AlwNI ApoI AspHI AvaI AvaII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI