(Circular) (Six-base) MAPSORT of: pFN21AE3230.seq Check: 3712 from: 1 to: 6313 pFN21AE3230 6313 bp With 182 enzymes: SgfI * March 28, 2012 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3440 3440 Size: 6313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3172 4123 278 Size: 53 83 186 936 1636 951 2468 Fragments arranged by size: 2468 1636 951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3540 3656 1937 Size: 1603 116 4594 Fragments arranged by size: 4594 1603 116 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2032 2363 4376 5760 6028 1632 Size: 400 331 2013 1384 268 1917 Fragments arranged by size: 2013 1917 1384 400 331 268 AfeI AGC'GCT Cuts at: 2798 2798 Size: 6313 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 5212 6189 1203 Size: 4009 977 1327 Fragments arranged by size: 4009 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 3004 1166 Size: 1838 4475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3022 1184 Size: 1838 4475 AlwNI CAG_nnn'CTG Cuts at: 2218 2338 2403 4056 5628 2218 Size: 120 65 1653 1572 2903 Fragments arranged by size: 2903 1653 1572 120 65 ApaLI G'TGCA_C Cuts at: 2165 2715 4370 5526 2165 Size: 550 1655 1156 2952 Fragments arranged by size: 2952 1655 1156 550 ApoI r'AATT_y Cuts at: 1493 2138 2538 2580 2662 3622 3848 4000 6109 Size: 645 400 42 82 960 226 152 2109 Cuts at: 6109 1493 Size: 1697 Fragments arranged by size: 2109 1697 960 645 400 226 152 82 42 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 2555 3523 3572 3597 3638 1870 Size: 73 612 968 49 25 41 4545 Fragments arranged by size: 4545 968 612 73 49 41 25 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2708 2903 3634 5089 618 Size: 325 217 627 921 195 731 1455 1842 Fragments arranged by size: 1842 1455 921 731 627 325 217 195 BanII G_rGCy'C Cuts at: 1391 2329 2940 3632 1391 Size: 938 611 692 4072 Fragments arranged by size: 4072 938 692 611 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 928 Size: 971 190 5152 Fragments arranged by size: 5152 971 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2003 2037 4504 4538 1903 Size: 34 66 34 2467 34 3678 Fragments arranged by size: 3678 2467 66 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3067 4205 5415 3067 Size: 1138 1210 3965 Fragments arranged by size: 3965 1210 1138 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2333 3407 4924 Size: 107 122 71 631 605 661 1074 1517 Cuts at: 4924 136 Size: 1525 Fragments arranged by size: 1525 1517 1074 661 631 605 122 107 71 BglII A'GATC_T Cuts at: 2128 6308 2128 Size: 4180 2133 BlpI GC'TnA_GC Cuts at: 2738 3730 2738 Size: 992 5321 Bme1580I G_kGCm'C Cuts at: 1790 2169 2719 2906 3468 4374 5530 1790 Size: 379 550 187 562 906 1156 2573 Fragments arranged by size: 2573 1156 906 562 550 379 187 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2431 2847 3553 Size: 416 53 83 399 24 1014 416 706 Cuts at: 3553 4044 5002 442 Size: 491 958 1753 Fragments arranged by size: 1753 1014 958 706 491 416 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2178 2343 2407 2471 2796 2894 3088 4973 Size: 939 165 64 64 325 98 194 1885 Cuts at: 4973 1239 Size: 2579 Fragments arranged by size: 2579 1885 939 325 194 165 98 64 64 Bpu10I CC'TnA_GC Cuts at: 2472 2472 Size: 6313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2250 4438 5303 5601 2250 Size: 2188 865 298 2962 Fragments arranged by size: 2962 2188 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4976 882 Size: 617 3477 2219 Fragments arranged by size: 3477 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3169 4120 4502 5137 Size: 53 83 186 936 1636 951 382 635 Cuts at: 5137 275 Size: 1451 Fragments arranged by size: 1636 1451 951 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3607 4746 5144 5418 5565 5976 3607 Size: 1139 398 274 147 411 3944 Fragments arranged by size: 3944 1139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2343 2373 2375 2405 1178 Size: 30 165 30 940 30 2 30 5086 Fragments arranged by size: 5086 940 165 30 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2744 3339 1186 Size: 1558 595 4160 Fragments arranged by size: 4160 1558 595 BseYI C'CCAG_C Cuts at: 2040 2328 2848 2985 5516 6012 2040 Size: 288 520 137 2531 496 2341 Fragments arranged by size: 2531 2341 520 496 288 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5740 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2169 2473 2719 3235 4374 4459 5530 2016 Size: 153 304 246 516 1139 85 1071 2799 Fragments arranged by size: 2799 1139 1071 516 304 246 153 85 BsmI GAATG_Cn' Cuts at: 3206 3824 3917 3206 Size: 618 93 5602 Fragments arranged by size: 5602 618 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2169 2329 2473 2719 2906 2940 Size: 399 226 153 160 144 246 187 34 Cuts at: 2940 3235 3468 3632 4374 4459 5530 1391 Size: 295 233 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 246 233 226 187 164 160 153 144 85 34 BspEI T'CCGG_A Cuts at: 3607 5976 3607 Size: 2369 3944 BspHI T'CATG_A Cuts at: 3027 4202 5932 3027 Size: 1175 1730 3408 Fragments arranged by size: 3408 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 3440 3668 844 Size: 1206 1390 228 3489 Fragments arranged by size: 3489 1390 1206 228 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2886 4808 4982 65 Size: 1183 1638 1922 174 1396 Fragments arranged by size: 1922 1638 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 4373 5385 4373 Size: 1012 5301 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 3103 3103 Size: 6313 BstYI r'GATC_y Cuts at: 1972 2128 3039 3643 4010 4395 4412 5864 5950 Size: 156 911 604 367 385 17 1452 86 Cuts at: 5950 5962 6308 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 911 604 385 367 346 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 3086 3396 3829 4623 4643 3086 Size: 310 433 794 20 4756 Fragments arranged by size: 4756 794 433 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6278 DraI TTT'AAA Cuts at: 3013 3617 3013 Size: 604 5709 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 5320 809 Size: 507 1575 2429 1802 Fragments arranged by size: 2429 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3255 3315 3760 Size: 54 1425 50 275 18 1425 60 445 Cuts at: 3760 4649 6278 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1425 889 445 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4243 1353 Size: 2890 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4900 5274 5420 387 Size: 1064 138 435 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2178 2343 2363 2407 2471 2796 Size: 393 400 146 165 20 44 64 325 Cuts at: 2796 2894 3088 4376 4973 5760 6028 1239 Size: 98 194 1288 597 787 268 1524 Fragments arranged by size: 1524 1288 787 597 400 393 325 268 194 165 146 98 64 44 20 EcoO109I rG'GnC_Cy Cuts at: 2496 3547 3602 2496 Size: 1051 55 5207 Fragments arranged by size: 5207 1051 55 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 EcoRV GAT'ATC Cuts at: 2224 2224 Size: 6313 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 2800 5460 1678 Size: 1122 2660 2531 Fragments arranged by size: 2660 2531 1122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 3024 3041 3056 Size: 32 135 32 803 32 617 17 15 Cuts at: 3056 3073 4954 4986 5028 5060 1373 Size: 17 1881 32 42 32 2626 Fragments arranged by size: 2626 1881 803 617 135 42 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2980 3657 3903 669 Size: 988 281 1042 677 246 3079 Fragments arranged by size: 3079 1042 988 677 281 246 HindIII A'AGCT_T Cuts at: 748 2344 2735 748 Size: 1596 391 4326 Fragments arranged by size: 4326 1596 391 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 5611 1305 Size: 4122 184 2007 Fragments arranged by size: 4122 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3257 3317 10 Size: 54 1425 343 1425 60 3006 Fragments arranged by size: 3006 1425 1425 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 3004 3101 4271 4630 4789 518 Size: 648 1006 832 97 1170 359 159 2042 Fragments arranged by size: 2042 1170 1006 832 648 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2028 2449 2484 3729 4406 5554 5799 1606 Size: 422 421 35 1245 677 1148 245 2120 Fragments arranged by size: 2120 1245 1148 677 422 421 245 35 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 3671 5216 1132 Size: 97 2442 1545 2229 Fragments arranged by size: 2442 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3212 1348 Size: 1864 4449 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 2141 2218 1108 Size: 1033 77 5203 Fragments arranged by size: 5203 1033 77 PfoI T'CCnGG_A Cuts at: 2812 3065 2812 Size: 253 6060 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2496 2496 Size: 6313 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspXI vC'TCGA_Gb Cuts at: 3572 3572 Size: 6313 PstI C_TGCA'G Cuts at: 830 3093 3665 830 Size: 2263 572 3478 Fragments arranged by size: 3478 2263 572 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 2449 2484 2449 Size: 35 6278 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SanDI GG'GwC_CC Cuts at: 2496 2496 Size: 6313 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 2199 4561 6273 2199 Size: 2362 1712 2239 Fragments arranged by size: 2362 2239 1712 SfcI C'TryA_G Cuts at: 826 1045 2641 3089 3661 4796 5477 5668 826 Size: 219 1596 448 572 1135 681 191 1471 Fragments arranged by size: 1596 1471 1135 681 572 448 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3525 3599 3640 1872 Size: 1653 74 41 4545 Fragments arranged by size: 4545 1653 74 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 2265 3572 4417 5318 5580 820 Size: 197 926 322 1307 845 901 262 1553 Fragments arranged by size: 1553 1307 926 901 845 322 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 2561 3279 1517 Size: 1044 718 4551 Fragments arranged by size: 4551 1044 718 StyI C'CwwG_G Cuts at: 513 1065 2004 2617 2704 3752 513 Size: 552 939 613 87 1048 3074 Fragments arranged by size: 3074 1048 939 613 552 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 4340 4357 4510 4695 5140 325 Size: 1255 873 1887 17 153 185 445 1498 Fragments arranged by size: 1887 1498 1255 873 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2197 4559 6271 Size: 274 80 33 51 1443 220 2362 1712 Cuts at: 6271 96 Size: 138 Fragments arranged by size: 2362 1712 1443 274 220 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2343 2375 2808 2840 3447 3479 6273 6305 2343 Size: 32 433 32 607 32 2794 32 2351 Fragments arranged by size: 2794 2351 607 433 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2202 2702 2947 2202 Size: 500 245 5568 Fragments arranged by size: 5568 500 245 XhoI C'TCGA_G Cuts at: 1943 3572 1943 Size: 1629 4684 XmaI C'CCGG_G Cuts at: 1870 3523 3597 3638 1870 Size: 1653 74 41 4545 Fragments arranged by size: 4545 1653 74 41 XmnI GAAnn'nnTTC Cuts at: 2096 4442 2096 Size: 2346 3967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3170 4121 276 Size: 53 83 186 936 1636 951 2468 Fragments arranged by size: 2468 1636 951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI