(Linear) (Six-base) MAPSORT of: KIEE3230.seq Check: 9800 from: 1 to: 1608 KIEE3230.orf With 182 enzymes: SgfI * March 22, 2012 16:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1435 1608 Size: 1435 173 AatII G_ACGT'C Cuts at: 0 1167 1608 Size: 1167 441 AccI GT'mk_AC Cuts at: 0 1535 1608 Size: 1535 73 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 358 1608 Size: 27 331 1250 Fragments arranged by size: 1250 331 27 AfeI AGC'GCT Cuts at: 0 793 1608 Size: 793 815 AleI CACnn'nnGTG Cuts at: 0 999 1608 Size: 999 609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1017 1608 Size: 1017 591 AlwNI CAG_nnn'CTG Cuts at: 0 213 333 398 1608 Size: 213 120 65 1210 Fragments arranged by size: 1210 213 120 65 ApaLI G'TGCA_C Cuts at: 0 160 710 1608 Size: 160 550 898 Fragments arranged by size: 898 550 160 ApoI r'AATT_y Cuts at: 0 133 533 575 657 1608 Size: 133 400 42 82 951 Fragments arranged by size: 951 400 133 82 42 AvaI C'yCGr_G Cuts at: 0 550 1518 1567 1592 1608 Size: 550 968 49 25 16 Fragments arranged by size: 968 550 49 25 16 BanI G'GyrC_C Cuts at: 0 703 898 1608 Size: 703 195 710 Fragments arranged by size: 710 703 195 BanII G_rGCy'C Cuts at: 0 324 935 1608 Size: 324 611 673 Fragments arranged by size: 673 611 324 BbsI GAAGACnn'nnnn_ Cuts at: 0 84 1608 Size: 84 1524 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 32 1608 Size: 32 1576 BciVI GTATCCnnnnn_n' Cuts at: 0 1062 1608 Size: 1062 546 BglI GCCn_nnn'nGGC Cuts at: 0 328 1402 1608 Size: 328 1074 206 Fragments arranged by size: 1074 328 206 BglII A'GATC_T Cuts at: 0 123 1608 Size: 123 1485 BlpI GC'TnA_GC Cuts at: 0 733 1608 Size: 733 875 Bme1580I G_kGCm'C Cuts at: 0 164 714 901 1463 1608 Size: 164 550 187 562 145 Fragments arranged by size: 562 550 187 164 145 BmrI ACTGGGnnnn_n' Cuts at: 0 426 842 1548 1608 Size: 426 416 706 60 Fragments arranged by size: 706 426 416 60 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 576 1608 Size: 576 1032 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 338 402 466 791 889 1083 1608 Size: 173 165 64 64 325 98 194 525 Fragments arranged by size: 525 325 194 173 165 98 64 64 Bpu10I CC'TnA_GC Cuts at: 0 467 1608 Size: 467 1141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 1608 Size: 245 1363 BsaHI Gr'CG_yC Cuts at: 0 1164 1608 Size: 1164 444 BsaWI w'CCGG_w Cuts at: 0 1602 1608 Size: 1602 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 338 368 370 400 1608 Size: 338 30 2 30 1208 Fragments arranged by size: 1208 338 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 739 1334 1608 Size: 739 595 274 Fragments arranged by size: 739 595 274 BseYI C'CCAG_C Cuts at: 0 35 323 843 980 1608 Size: 35 288 520 137 628 Fragments arranged by size: 628 520 288 137 35 BsiHKAI G_wGCw'C Cuts at: 0 11 164 468 714 1230 1608 Size: 11 153 304 246 516 378 Fragments arranged by size: 516 378 304 246 153 11 BsmI GAATG_Cn' Cuts at: 0 1201 1608 Size: 1201 407 Bsp1286I G_dGCh'C Cuts at: 0 11 164 324 468 714 901 935 1230 Size: 11 153 160 144 246 187 34 295 Cuts at: 1230 1463 1608 Size: 233 145 Fragments arranged by size: 295 246 233 187 160 153 145 144 34 11 BspEI T'CCGG_A Cuts at: 0 1602 1608 Size: 1602 6 BspHI T'CATG_A Cuts at: 0 1022 1608 Size: 1022 586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 45 1435 1608 Size: 45 1390 173 Fragments arranged by size: 1390 173 45 BsrDI GCAATG_nn' Cuts at: 0 881 1608 Size: 881 727 BstXI CCAn_nnnn'nTGG Cuts at: 0 1098 1608 Size: 1098 510 BstYI r'GATC_y Cuts at: 0 123 1034 1608 Size: 123 911 574 Fragments arranged by size: 911 574 123 BtsI GCAGTG_nn' Cuts at: 0 1081 1391 1608 Size: 1081 310 217 Fragments arranged by size: 1081 310 217 DraI TTT'AAA Cuts at: 0 1008 1608 Size: 1008 600 DrdI GACnn_nn'nnGTC Cuts at: 0 886 1608 Size: 886 722 EaeI y'GGCC_r Cuts at: 0 1250 1310 1608 Size: 1250 60 298 Fragments arranged by size: 1250 298 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1608 Size: 19 1589 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 173 338 358 402 466 791 889 Size: 27 146 165 20 44 64 325 98 Cuts at: 889 1083 1608 Size: 194 525 Fragments arranged by size: 525 325 194 165 146 98 64 44 27 20 EcoO109I rG'GnC_Cy Cuts at: 0 491 1542 1597 1608 Size: 491 1051 55 11 Fragments arranged by size: 1051 491 55 11 EcoRV GAT'ATC Cuts at: 0 219 1608 Size: 219 1389 HaeII r_GCGC'y Cuts at: 0 795 1608 Size: 795 813 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 370 402 1019 1036 1051 1068 1608 Size: 370 32 617 17 15 17 540 Fragments arranged by size: 617 540 370 32 17 17 15 HincII GTy'rAC Cuts at: 0 975 1608 Size: 975 633 HindIII A'AGCT_T Cuts at: 0 339 730 1608 Size: 339 391 878 Fragments arranged by size: 878 391 339 MscI TGG'CCA Cuts at: 0 1252 1312 1608 Size: 1252 60 296 Fragments arranged by size: 1252 296 60 MslI CAynn'nnrTG Cuts at: 0 167 999 1096 1608 Size: 167 832 97 512 Fragments arranged by size: 832 512 167 97 MspA1I CmG'CkG Cuts at: 0 23 444 479 1608 Size: 23 421 35 1129 Fragments arranged by size: 1129 421 35 23 PasI CC'CwG_GG Cuts at: 0 1207 1608 Size: 1207 401 PflMI CCAn_nnn'nTGG Cuts at: 0 136 213 1608 Size: 136 77 1395 Fragments arranged by size: 1395 136 77 PfoI T'CCnGG_A Cuts at: 0 807 1060 1608 Size: 807 253 548 Fragments arranged by size: 807 548 253 PpuMI rG'GwC_Cy Cuts at: 0 491 1608 Size: 491 1117 PspXI vC'TCGA_Gb Cuts at: 0 1567 1608 Size: 1567 41 PstI C_TGCA'G Cuts at: 0 1088 1608 Size: 1088 520 PvuII CAG'CTG Cuts at: 0 444 479 1608 Size: 444 35 1129 Fragments arranged by size: 1129 444 35 SanDI GG'GwC_CC Cuts at: 0 491 1608 Size: 491 1117 ScaI AGT'ACT Cuts at: 0 194 1608 Size: 194 1414 SfcI C'TryA_G Cuts at: 0 636 1084 1608 Size: 636 448 524 Fragments arranged by size: 636 524 448 SmaI CCC'GGG Cuts at: 0 1520 1594 1608 Size: 1520 74 14 Fragments arranged by size: 1520 74 14 SmlI C'TyrA_G Cuts at: 0 260 1567 1608 Size: 260 1307 41 Fragments arranged by size: 1307 260 41 StuI AGG'CCT Cuts at: 0 556 1274 1608 Size: 556 718 334 Fragments arranged by size: 718 556 334 StyI C'CwwG_G Cuts at: 0 612 699 1608 Size: 612 87 909 Fragments arranged by size: 909 612 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 448 1608 Size: 448 1160 TatI w'GTAC_w Cuts at: 0 192 1608 Size: 192 1416 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 338 370 803 835 1442 1474 1608 Size: 338 32 433 32 607 32 134 Fragments arranged by size: 607 433 338 134 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 197 697 942 1608 Size: 197 500 245 666 Fragments arranged by size: 666 500 245 197 XhoI C'TCGA_G Cuts at: 0 1567 1608 Size: 1567 41 XmaI C'CCGG_G Cuts at: 0 1518 1592 1608 Size: 1518 74 16 Fragments arranged by size: 1518 74 16 XmnI GAAnn'nnTTC Cuts at: 0 91 1608 Size: 91 1517 ZraI GAC'GTC Cuts at: 0 1165 1608 Size: 1165 443 Enzymes that do cut: AarI AatII AccI AcuI AfeI AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BcgI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstXI BstYI BtsI DraI DrdI EaeI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII MscI MslI MspA1I PasI PflMI PfoI PpuMI PspXI PstI PvuII SanDI ScaI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BmgBI BmtI BsaI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII EagI EarI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI