##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstNI       
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MboI        MflI        MluI        MluNI       
MroI        MscI        MslI        MvaI        NarI        
NcoI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         115  CGCG                 CG^CG
ApaI           45  GGGCCC               GGGCC^C
AvaI           42  CCCGGG               C^[CT]CG[GA]G
AvaII          39  GGACC                G^G[AT]CC
BanII          45  GGGCCC               G[GA]GC[CT]^C
BfaI          515  CTAG                 C^TAG
BmyI           45  GGGCCC               G[AGT]GC[ACT]^C
BseRI          34  GAGGAG               GAGGAG(10/8)
BsmFI         486  GTCCC                GGGAC(10/14)
BsoBI          42  CCCGGG               C^[CT]CG[GA]G
Bsp1286I       45  GGGCCC               G[AGT]GC[ACT]^C
Bsp1407I       17  TGTACA               T^GTACA
BsrDI         104  CATTGC               GCAATG(2/0)
BsrFI          95  GCCGGC               [GA]^CCGG[CT]
BsrGI          17  TGTACA               T^GTACA
BstAPI        132  GCAGCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         115  CGCG                 CG^CG
CfoI          114  GCGC                 GCG^C
Cfr10I         95  GCCGGC               [GA]^CCGG[CT]
FauI           49  CCCGC                CCCGC(4/6)
HhaI          114  GCGC                 GCG^C
HinP1I        114  GCGC                 G^CGC
MaeI          515  CTAG                 C^TAG
MfeI          410  CAATTG               C^AATTG
MseI          542  TTAA                 T^TAA
MunI          410  CAATTG               C^AATTG
MvnI          115  CGCG                 CG^CG
NaeI           95  GCCGGC               GCC^GGC
NgoMIV         95  GCCGGC               G^CCGGC
PpuMI          38  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         479  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          225  CTGCAG               CTGCA^G
SfaNI         358  GCATC                GCATC(5/9)
SfcI          225  CTGCAG               C^T[GA][CT]AG
SmaI           42  CCCGGG               CCC^GGG
SmlI          121  CTCAAG               C^T[CT][GA]AG
SspBI          17  TGTACA               T^GTACA
Tru9I         542  TTAA                 T^TAA
Tsp509I       411  AATT                 ^AATT
XmaCI          42  CCCGGG               C^CCGGG
XmaI           42  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI           18  GTAC                 GT^AC
              185  GTAC                
BsaJI          42  CCCGGG               C^C[ACGT][ACGT]GG
               61  CCCCGG              
BsmAI         144  GTCTC                GTCTC(1/5)
              245  GTCTC               
BsrBI          11  GAGCGG               CCGCTC(-3/-3)
               66  GAGCGG              
BstF5I        359  CATCC                GGATG(2/0)
              340  GGATG               
FokI          359  CATCC                GGATG(9/13)
              340  GGATG               
HinfI         243  GAGTC                G^A[ACGT]TC
              484  GAGTC               
MboII         166  GAAGA                GAAGA(8/7)
              237  TCTTC               
NlaIII         90  CATG                 CATG^
              382  CATG                
PleI          243  GAGTC                GAGTC(4/5)
              484  GAGTC               
RsaI           18  GTAC                 GT^AC
              185  GTAC                
TspRI         228  CAGTG                CA[GC]TG[ACGT][ACGT]^
              531  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsiYI       
BslI        BssKI       Cac8I       Cfr13I      DdeI        
DraII       EcoO109I    Fnu4HI      HaeIII      HapII       
HpaII       ItaI        MnlI        MspA1I      MspI        
MwoI        NciI        NlaIV       PvuII       Sau96I      
ScrFI       TseI