################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstF5I BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I FbaI FokI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 16 CGCG CG^CG AlwI 503 GATCC GGATC(4/5) BanI 303 GGTGCC G^G[CT][GA]CC BbsI 253 GTCTTC GAAGAC(2/6) BmyI 304 GTGCCC G[AGT]GC[ACT]^C BpuAI 253 GTCTTC GAAGAC(2/6) BseRI 4 GAGGAG GAGGAG(10/8) BsgI 118 CTGCAC GTGCAG(16/14) BsiEI 96 CGACCG CG[GA][CT]^CG BsmAI 388 GTCTC GTCTC(1/5) BsmBI 387 CGTCTC CGTCTC(1/5) Bsp1286I 304 GTGCCC G[AGT]GC[ACT]^C BsrBI 21 CCGCTC CCGCTC(-3/-3) BsrI 239 ACTGG ACTGG(1/-1) BssHII 15 GCGCGC G^CGCGC BstEII 453 GGTCACC G^GT[ACGT]ACC BstPI 453 GGTCACC G^GT[ACGT]ACC BstUI 16 CGCG CG^CG BstYI 502 AGATCC [GA]^GATC[CT] EaeI 137 TGGCCG [CT]^GGCC[GA] EcoNI 284 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 453 GGTCACC G^GT[ACGT]ACC EcoT22I 377 ATGCAT ATGCA^T FauI 20 CCCGC CCCGC(4/6) HgaI 168 GACGC GACGC(5/10) HinfI 294 GATTC G^A[ACGT]TC MflI 502 AGATCC [GA]^GATC[CT] MseI 332 TTAA T^TAA MslI 273 CATAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 16 CGCG CG^CG NsiI 377 ATGCAT ATGCA^T PflMI 202 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 420 CAGCTG CAG^CTG ScaI 188 AGTACT AGT^ACT TfiI 294 GATTC G^A[AT]TC Tru9I 332 TTAA T^TAA Van91I 202 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 202 CCAGCATTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 502 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 189 GTAC GT^AC 354 GTAC BcgI 80 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 338 GCAGTGCCTTCG BcnI 99 CCGGG CC^[GC]GG 195 CCGGG BsaJI 299 CCCAGG C^C[ACGT][ACGT]GG 307 CCCTGG BsmFI 401 GGGAC GGGAC(10/14) 199 GTCCC CfoI 15 GCGC GCG^C 17 GCGC DdeI 185 CTGAG C^T[ACGT]AG 395 CTGAG DpnII 144 GATC ^GATC 503 GATC DraII 197 GGGTCCC [GA]G^G[ACGT]CC[CT] 242 GGGTCCT EcoO109I 197 GGGTCCC [GA]G^G[ACGT]CC[CT] 242 GGGTCCT HapII 99 CCGG C^CGG 195 CCGG HhaI 15 GCGC GCG^C 17 GCGC HinP1I 15 GCGC G^CGC 17 GCGC HpaII 99 CCGG C^CGG 195 CCGG MboI 144 GATC ^GATC 503 GATC MspA1I 365 CCGCTG C[AC]G^C[GT]G 420 CAGCTG MspI 99 CCGG C^CGG 195 CCGG MwoI 77 GCAGCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 329 GCTTTAACGGC NciI 99 CCGGG CC^[GC]GG 195 CCGGG NdeII 144 GATC ^GATC 503 GATC NlaIII 219 CATG CATG^ 497 CATG PpuMI 197 GGGTCCC [GA]G^G[AT]CC[CT] 242 GGGTCCT RsaI 189 GTAC GT^AC 354 GTAC Sau3AI 144 GATC ^GATC 503 GATC SfaNI 376 GATGC GCATC(5/9) 379 GCATC TspRI 317 CAGTG CA[GC]TG[ACGT][ACGT]^ 339 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI AvaII BbvI BsiYI BslI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboII MnlI MvaI NlaIV Sau96I ScrFI TseI Tsp45I