(Linear) (Six-base) MAPSORT of: KIEE3038.seq  Check: 5138  from: 1  to: 519



KIEE3038.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:05  ..

BanI G'GyrC_C

Cuts at:      0     303     519 
   Size:        303     216
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     246     519 
   Size:        246     273
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      69     103     327     361     519 
   Size:         69      34     224      34     158

  Fragments arranged by size: 

                 224     158      69      34      34
 
BfrBI ATG'CAT

Cuts at:      0     379     519 
   Size:        379     140
 
Bme1580I G_kGCm'C

Cuts at:      0     308     519 
   Size:        308     211
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     249     519 
   Size:        249     270
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      84     519 
   Size:         84     435
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     519 
   Size:         19     500
 
BseYI C'CCAG_C

Cuts at:      0     201     212     422     519 
   Size:        201      11     210      97

  Fragments arranged by size: 

                 210     201      97      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     519 
   Size:        103     416
 
BsiEI CG_ry'CG

Cuts at:      0      99     519 
   Size:         99     420
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     393     519 
   Size:        393     126
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     519 
   Size:        308     211
 
BsrBI CCG'CTC

Cuts at:      0      23     519 
   Size:         23     496
 
BssHII G'CGCG_C

Cuts at:      0      15     519 
   Size:         15     504
 
BstEII G'GTnAC_C

Cuts at:      0     453     519 
   Size:        453      66
 
BstYI r'GATC_y

Cuts at:      0     502     519 
   Size:        502      17
 
BtsI GCAGTG_nn'

Cuts at:      0     345     519 
   Size:        345     174
 
EaeI y'GGCC_r

Cuts at:      0     137     519 
   Size:        137     382
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     288     519 
   Size:        288     231
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198     243     519 
   Size:        198      45     276

  Fragments arranged by size: 

                 276     198      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     390     422     519 
   Size:        390      32      97

  Fragments arranged by size: 

                 390      97      32
 
MslI CAynn'nnrTG

Cuts at:      0     277     519 
   Size:        277     242
 
MspA1I CmG'CkG

Cuts at:      0     367     422     519 
   Size:        367      55      97

  Fragments arranged by size: 

                 367      97      55
 
NsiI A_TGCA'T

Cuts at:      0     381     519 
   Size:        381     138
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     208     519 
   Size:        208     311
 
PfoI T'CCnGG_A

Cuts at:      0     505     519 
   Size:        505      14
 
PpuMI rG'GwC_Cy

Cuts at:      0     198     243     519 
   Size:        198      45     276

  Fragments arranged by size: 

                 276     198      45
 
PvuII CAG'CTG

Cuts at:      0     422     519 
   Size:        422      97
 
SanDI GG'GwC_CC

Cuts at:      0     198     519 
   Size:        198     321
 
ScaI AGT'ACT

Cuts at:      0     190     519 
   Size:        190     329
 
TatI w'GTAC_w

Cuts at:      0     188     519 
   Size:        188     331
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     279     311     519 
   Size:        279      32     208

  Fragments arranged by size: 

                 279     208      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     209     519 
   Size:        209     310
 

 Enzymes that do cut:

     BanI     BbsI     BcgI    BfrBI Bme1580I     BmrI     BplI    BseRI
    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BsrBI   BssHII   BstEII
    BstYI     BtsI     EaeI    EcoNI EcoO109I    Hin4I     MslI   MspA1I
     NsiI    PflMI     PfoI    PpuMI    PvuII    SanDI     ScaI     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI