Restriction map for
pFN21AB5810
(Linear) (Six-base) MAPSORT of: KIBB5810.seq Check: 4634 from: 1 to: 1344
KIBB5810.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 408 808 1344
Size: 408 400 536
Fragments arranged by size:
536 408 400
Acc65I G'GTAC_C
Cuts at: 0 269 1344
Size: 269 1075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 781 1250 1344
Size: 93 688 469 94
Fragments arranged by size:
688 469 94 93
AflIII A'CryG_T
Cuts at: 0 1094 1344
Size: 1094 250
AlwNI CAG_nnn'CTG
Cuts at: 0 290 927 1344
Size: 290 637 417
Fragments arranged by size:
637 417 290
ApoI r'AATT_y
Cuts at: 0 609 658 794 1058 1181 1224 1344
Size: 609 49 136 264 123 43 120
Fragments arranged by size:
609 264 136 123 120 49 43
AseI AT'TA_AT
Cuts at: 0 374 1344
Size: 374 970
AvaI C'yCGr_G
Cuts at: 0 224 1344
Size: 224 1120
BanI G'GyrC_C
Cuts at: 0 269 1344
Size: 269 1075
BbsI GAAGACnn'nnnn_
Cuts at: 0 62 1344
Size: 62 1282
BglII A'GATC_T
Cuts at: 0 1106 1125 1344
Size: 1106 19 219
Fragments arranged by size:
1106 219 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 397 1240 1344
Size: 67 330 843 104
Fragments arranged by size:
843 330 104 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1344
Size: 1024 320
BsaI GGTCTCn'nnnn_
Cuts at: 0 983 1344
Size: 983 361
BseYI C'CCAG_C
Cuts at: 0 591 1081 1297 1344
Size: 591 490 216 47
Fragments arranged by size:
591 490 216 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1172 1344
Size: 1172 172
BsiHKAI G_wGCw'C
Cuts at: 0 175 1280 1344
Size: 175 1105 64
Fragments arranged by size:
1105 175 64
BsmI GAATG_Cn'
Cuts at: 0 502 1344
Size: 502 842
Bsp1286I G_dGCh'C
Cuts at: 0 175 1280 1344
Size: 175 1105 64
Fragments arranged by size:
1105 175 64
BsrDI GCAATG_nn'
Cuts at: 0 1039 1344
Size: 1039 305
BsrGI T'GTAC_A
Cuts at: 0 230 1344
Size: 230 1114
BssSI C'ACGA_G
Cuts at: 0 1336 1344
Size: 1336 8
BstBI TT'CG_AA
Cuts at: 0 487 1344
Size: 487 857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 1344
Size: 1305 39
BstYI r'GATC_y
Cuts at: 0 548 1106 1125 1344
Size: 548 558 19 219
Fragments arranged by size:
558 548 219 19
BstZ17I GTA'TAC
Cuts at: 0 809 1344
Size: 809 535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 723 1344
Size: 723 621
BtsI GCAGTG_nn'
Cuts at: 0 56 146 1344
Size: 56 90 1198
Fragments arranged by size:
1198 90 56
ClaI AT'CG_AT
Cuts at: 0 393 1344
Size: 393 951
DraIII CAC_nnn'GTG
Cuts at: 0 680 1344
Size: 680 664
DrdI GACnn_nn'nnGTC
Cuts at: 0 628 1344
Size: 628 716
EaeI y'GGCC_r
Cuts at: 0 671 1344
Size: 671 673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 93 397 781 1240 1250 1344
Size: 67 26 304 384 459 10 94
Fragments arranged by size:
459 384 304 94 67 26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 1344
Size: 760 584
EcoO109I rG'GnC_Cy
Cuts at: 0 999 1344
Size: 999 345
EcoRI G'AATT_C
Cuts at: 0 609 1224 1344
Size: 609 615 120
Fragments arranged by size:
615 609 120
HaeII r_GCGC'y
Cuts at: 0 290 1344
Size: 290 1054
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 247 279 1344
Size: 7 32 208 32 1065
Fragments arranged by size:
1065 208 32 32 7
HincII GTy'rAC
Cuts at: 0 409 1344
Size: 409 935
KpnI G_GTAC'C
Cuts at: 0 273 1344
Size: 273 1071
MfeI C'AATT_G
Cuts at: 0 158 1344
Size: 158 1186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 657 1344
Size: 509 148 687
Fragments arranged by size:
687 509 148
MscI TGG'CCA
Cuts at: 0 673 1344
Size: 673 671
MspA1I CmG'CkG
Cuts at: 0 523 591 1344
Size: 523 68 753
Fragments arranged by size:
753 523 68
NspI r_CATG'y
Cuts at: 0 81 107 1098 1344
Size: 81 26 991 246
Fragments arranged by size:
991 246 81 26
PciI A'CATG_T
Cuts at: 0 1094 1344
Size: 1094 250
PfoI T'CCnGG_A
Cuts at: 0 44 83 1185 1344
Size: 44 39 1102 159
Fragments arranged by size:
1102 159 44 39
PpuMI rG'GwC_Cy
Cuts at: 0 999 1344
Size: 999 345
PspXI vC'TCGA_Gb
Cuts at: 0 224 1344
Size: 224 1120
PvuII CAG'CTG
Cuts at: 0 523 591 1344
Size: 523 68 753
Fragments arranged by size:
753 523 68
SalI G'TCGA_C
Cuts at: 0 407 1344
Size: 407 937
SexAI A'CCwGG_T
Cuts at: 0 802 1344
Size: 802 542
SmlI C'TyrA_G
Cuts at: 0 224 1003 1344
Size: 224 779 341
Fragments arranged by size:
779 341 224
SphI G_CATG'C
Cuts at: 0 81 107 1344
Size: 81 26 1237
Fragments arranged by size:
1237 81 26
StuI AGG'CCT
Cuts at: 0 959 1344
Size: 959 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1311 1344
Size: 1311 33
TatI w'GTAC_w
Cuts at: 0 230 1344
Size: 230 1114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 48 80 1344
Size: 48 32 1264
Fragments arranged by size:
1264 48 32
XhoI C'TCGA_G
Cuts at: 0 224 1344
Size: 224 1120
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApoI AseI AvaI
BanI BbsI BglII BpmI BpuEI BsaI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrDI BsrGI BssSI BstBI BstXI
BstYI BstZ17I BtgZI BtsI ClaI DraIII DrdI EaeI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII KpnI
MfeI MmeI MscI MspA1I NspI PciI PfoI PpuMI
PspXI PvuII SalI SexAI SmlI SphI StuI TaqII
TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI
BstEII Bsu36I BtgI CspCI DraI EagI EarI EciI
EcoICRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StyI SwaI Tth111I XbaI XcmI XmaI XmnI
ZraI