################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FbaI Fnu4HI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TseI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 169 GCGG CCGC(-3/-1) AcsI 152 GAATTC [GA]^AATT[CT] ApoI 152 GAATTC [GA]^AATT[CT] BalI 548 TGGCCA TGG^CCA BamHI 262 GGATCC G^GATCC BbsI 48 GTCTTC GAAGAC(2/6) BfaI 250 CTAG C^TAG BpmI 257 CTCCAG CTGGAG(16/14) BpuAI 48 GTCTTC GAAGAC(2/6) BsaJI 491 CCTTGG C^C[ACGT][ACGT]GG BsmFI 209 GTCCC GGGAC(10/14) BssKI 259 CCAGG ^CC[ACGT]GG BstF5I 6 GGATG GGATG(2/0) BstNI 259 CCAGG CC^[AT]GG BstYI 262 GGATCC [GA]^GATC[CT] CfoI 143 GCGC GCG^C DdeI 512 CTCAG C^T[ACGT]AG DraII 252 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 548 TGGCCA [CT]^GGCC[GA] EcoO109I 252 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 152 GAATTC G^AATTC EcoRII 259 CCAGG ^CC[AT]GG EcoRV 295 GATATC GAT^ATC EcoT14I 491 CCTTGG C^C[AT][AT]GG FokI 6 GGATG GGATG(9/13) HaeII 142 AGCGCC [GA]GCGC^[CT] HhaI 143 GCGC GCG^C HinP1I 143 GCGC G^CGC MaeI 250 CTAG C^TAG MaeIII 337 GTTAC ^GT[ACGT]AC MflI 262 GGATCC [GA]^GATC[CT] MluNI 548 TGGCCA TGG^CCA MscI 548 TGGCCA TGG^CCA MseI 371 TTAA T^TAA MvaI 259 CCAGG CC^[AT]GG PflMI 551 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 417 GAGTC GAGTC(4/5) ScrFI 259 CCAGG CC^[ACGT]GG SspI 367 AATATT AAT^ATT StyI 491 CCTTGG C^C[AT][AT]GG Tru9I 371 TTAA T^TAA Van91I 551 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 262 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 31 AGCT AG^CT 164 AGCT AlwI 263 GATCC GGATC(4/5) 262 GGATC AvaII 208 GGTCC G^G[AT]CC 560 GGACC Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC 165 GCTGGC DpnII 239 GATC ^GATC 263 GATC MboI 239 GATC ^GATC 263 GATC NdeII 239 GATC ^GATC 263 GATC NlaIII 111 CATG CATG^ 538 CATG NlaIV 208 GGTCCC GG[ACGT]^[ACGT]CC 262 GGATCC Sau3AI 239 GATC ^GATC 263 GATC TaqI 16 TCGA T^CGA 293 TCGA TfiI 13 GATTC G^A[AT]TC 353 GAATC TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^ 556 CAGTG TthHB8I 16 TCGA T^CGA 293 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### BseRI BsiYI BslI Cfr13I HaeIII HinfI MboII MnlI Sau96I Tsp509I