################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MboII MfeI MluI MroI MslI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 176 GAATTC [GA]^AATT[CT] AlwI 286 GGATC GGATC(4/5) AlwNI 284 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 355 CCTAAGG CC^T[ACGT]AGG ApoI 176 GAATTC [GA]^AATT[CT] BalI 572 TGGCCA TGG^CCA BpmI 438 CTGGAG CTGGAG(16/14) BsaBI 58 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 566 ACCGGA [AT]^CCGG[AT] BsmAI 242 GAGAC GTCTC(1/5) BsmFI 276 GGGAC GGGAC(10/14) BsrI 437 ACTGG ACTGG(1/-1) BssKI 283 CCAGG ^CC[ACGT]GG BstEII 360 GGTTACC G^GT[ACGT]ACC BstF5I 569 GGATG GGATG(2/0) BstNI 283 CCAGG CC^[AT]GG BstPI 360 GGTTACC G^GT[ACGT]ACC BstYI 286 GGATCT [GA]^GATC[CT] Bsu36I 355 CCTAAGG CC^T[ACGT]AGG DraII 276 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 572 TGGCCA [CT]^GGCC[GA] Eco81I 355 CCTAAGG CC^T[ACGT]AGG EcoO109I 276 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 360 GGTTACC G^GT[ACGT]ACC EcoRI 176 GAATTC G^AATTC EcoRII 283 CCAGG ^CC[AT]GG EcoT14I 402 CCAAGG C^C[AT][AT]GG FokI 569 GGATG GGATG(9/13) HaeIII 573 GGCC GG^CC HphI 331 TCACC GGTGA(8/7) MaeII 69 ACGT A^CGT MaeIII 361 GTTAC ^GT[ACGT]AC MamI 58 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 286 GGATCT [GA]^GATC[CT] MluNI 572 TGGCCA TGG^CCA MscI 572 TGGCCA TGG^CCA MspA1I 146 CCGCTG C[AC]G^C[GT]G MvaI 283 CCAGG CC^[AT]GG PflMI 575 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 441 GAGTC GAGTC(4/5) PpuMI 276 GGGACCC [GA]G^G[AT]CC[CT] ScrFI 283 CCAGG CC^[ACGT]GG SfaNI 527 GCATC GCATC(5/9) SfcI 222 CTGTAG C^T[GA][CT]AG SspI 391 AATATT AAT^ATT StyI 402 CCAAGG C^C[AT][AT]GG TfiI 377 GAATC G^A[AT]TC Van91I 575 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 286 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 146 CCGC CCGC(-3/-1) 591 GCGG AfaI 540 GTAC GT^AC 594 GTAC Asp700I 73 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 100 GAACAAGTTC BbvI 524 GCAGC GCAGC(8/12) 148 GCTGC BfaI 41 CTAG C^TAG 164 CTAG BsaJI 282 CCCAGG C^C[ACGT][ACGT]GG 402 CCAAGG BseRI 212 CTCCTC GAGGAG(10/8) 469 CTCCTC DdeI 8 CTGAG C^T[ACGT]AG 356 CTAAG Fnu4HI 148 GCTGC GC^[ACGT]GC 524 GCAGC HapII 445 CCGG C^CGG 567 CCGG HincII 159 GTCAAC GT[CT]^[GA]AC 264 GTCAAC HindII 159 GTCAAC GT[CT]^[GA]AC 264 GTCAAC HinfI 377 GAATC G^A[ACGT]TC 441 GAGTC HpaII 445 CCGG C^CGG 567 CCGG ItaI 148 GCTGC GC^[ACGT]GC 524 GCAGC MaeI 41 CTAG C^TAG 164 CTAG MspI 445 CCGG C^CGG 567 CCGG NlaIII 543 CATG CATG^ 562 CATG RsaI 540 GTAC GT^AC 594 GTAC SpeI 40 ACTAGT A^CTAGT 163 ACTAGT TseI 148 GCTGC G^C[AT]GC 524 GCAGC Tsp509I 155 AATT ^AATT 177 AATT XmnI 73 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 100 GAACAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AvaII BsiYI BslI Cfr13I DpnII MboI MnlI MseI NdeII NlaIV Sau3AI Sau96I Tru9I TspRI