################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 415 CCGC CCGC(-3/-1) AflIII 277 ACATGT A^C[GA][CT]GT Alw44I 624 GTGCAC G^TGCAC ApaLI 624 GTGCAC G^TGCAC AspHI 624 GTGCAC G[AT]GC[AT]^C BalI 2 TGGCCA TGG^CCA BanII 649 GAGCCC G[GA]GC[CT]^C BbvI 121 GCTGC GCAGC(8/12) BciVI 540 GTATCC GTATCC(6/5) BpmI 387 CTCCAG CTGGAG(16/14) BsaJI 5 CCAAGG C^C[ACGT][ACGT]GG BsgI 122 CTGCAC GTGCAG(16/14) BsiHKAI 624 GTGCAC G[AT]GC[AT]^C BsmBI 741 GAGACG CGTCTC(1/5) BsmFI 680 GGGAC GGGAC(10/14) BspLU11I 277 ACATGT A^CATGT BstBI 663 TTCGAA TT^CGAA BstF5I 243 CATCC GGATG(2/0) DraII 680 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EarI 674 GAAGAG CTCTTC(1/4) EcoO109I 680 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 708 GAATTC G^AATTC EcoT14I 5 CCAAGG C^C[AT][AT]GG EcoT22I 290 ATGCAT ATGCA^T Fnu4HI 121 GCTGC GC^[ACGT]GC FokI 243 CATCC GGATG(9/13) HaeIII 3 GGCC GG^CC HinfI 457 GAATC G^A[ACGT]TC ItaI 121 GCTGC GC^[ACGT]GC Ksp632I 674 GAAGAG CTCTTC(1/4) MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspA1I 250 CAGCTG C[AC]G^C[GT]G NsiI 290 ATGCAT ATGCA^T NspI 277 ACATGT [GA]CATG^[CT] NspV 663 TTCGAA TT^CGAA PpuMI 680 GGGACCC [GA]G^G[AT]CC[CT] PvuII 250 CAGCTG CAG^CTG SfaNI 14 GATGC GCATC(5/9) SfcI 247 CTACAG C^T[GA][CT]AG SfuI 663 TTCGAA TT^CGAA StyI 5 CCAAGG C^C[AT][AT]GG TaqI 664 TCGA T^CGA TfiI 457 GAATC G^A[AT]TC TseI 121 GCTGC G^C[AT]GC Tsp45I 498 GTCAC ^GT[GC]AC TthHB8I 664 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Asp700I 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 560 GAAGACTTTC AvaII 366 GGACC G^G[AT]CC 681 GGACC BbsI 46 GAAGAC GAAGAC(2/6) 560 GAAGAC BmyI 624 GTGCAC G[AGT]GC[ACT]^C 649 GAGCCC BpuAI 46 GAAGAC GAAGAC(2/6) 560 GAAGAC BsiYI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 597 CCCAGCAGTGG BslI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 597 CCCAGCAGTGG BsmAI 741 GAGAC GTCTC(1/5) 532 GTCTC Bsp1286I 624 GTGCAC G[AGT]GC[ACT]^C 649 GAGCCC BspMI 330 ACCTGC ACCTGC(4/8) 107 GCAGGT BssKI 342 CCAGG ^CC[ACGT]GG 445 CCAGG BstNI 342 CCAGG CC^[AT]GG 445 CCAGG Cac8I 592 GCAAGC GC[ACGT]^[ACGT]GC 631 GCCAGC Cfr13I 366 GGACC G^G[ACGT]CC 681 GGACC EcoRII 342 CCAGG ^CC[AT]GG 445 CCAGG EcoRV 238 GATATC GAT^ATC 520 GATATC MaeII 618 ACGT A^CGT 744 ACGT MaeIII 266 GTTAC ^GT[ACGT]AC 498 GTCAC MvaI 342 CCAGG CC^[AT]GG 445 CCAGG MwoI 592 GCAAGCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 626 GCACAGCCAGC NlaIII 128 CATG CATG^ 278 CATG Sau96I 366 GGACC G^G[ACGT]CC 681 GGACC ScrFI 342 CCAGG CC^[ACGT]GG 445 CCAGG TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^ 602 CAGTG XmnI 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 560 GAAGACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AcsI AluI ApoI DdeI HphI MboII MnlI NlaIV Tsp509I