################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 136 CGCG CG^CG AclI 429 AACGTT AA^CGTT AlwI 150 GGATC GGATC(4/5) BciVI 309 GGATAC GTATCC(6/5) BglI 115 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 598 CACGTA [CT]AC^GT[GA] BsgI 523 CTGCAC GTGCAG(16/14) BsmAI 277 GAGAC GTCTC(1/5) Bsp1407I 440 TGTACA T^GTACA BspHI 548 TCATGA T^CATGA BspMI 623 ACCTGC ACCTGC(4/8) BsrGI 440 TGTACA T^GTACA BstEII 78 GGTGACC G^GT[ACGT]ACC BstF5I 680 CATCC GGATG(2/0) BstPI 78 GGTGACC G^GT[ACGT]ACC BstUI 136 CGCG CG^CG CfoI 543 GCGC GCG^C DdeI 22 CTTAG C^T[ACGT]AG EcoO65I 78 GGTGACC G^GT[ACGT]ACC FokI 680 CATCC GGATG(9/13) HhaI 543 GCGC GCG^C HinP1I 543 GCGC G^CGC HindIII 557 AAGCTT A^AGCTT HphI 78 GGTGA GGTGA(8/7) MvnI 136 CGCG CG^CG PflMI 294 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 191 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 429 AACGTT AA^CGTT PvuII 88 CAGCTG CAG^CTG RcaI 548 TCATGA T^CATGA ScaI 287 AGTACT AGT^ACT SfiI 114 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 581 GCATGC GCATG^C SspBI 440 TGTACA T^GTACA StuI 674 AGGCCT AGG^CCT Tsp45I 79 GTGAC ^GT[GC]AC Van91I 294 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 597 CCACGTACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 85 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 88 CAGCTGCTG ApaI 122 GGGCCC GGGCC^C 569 GGGCCC BanI 374 GGCACC G^G[CT][GA]CC 610 GGCACC BcnI 125 CCCGG CC^[GC]GG 210 CCGGG EarI 574 CTCTTC CTCTTC(1/4) 283 GAAGAG EcoNI 198 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 327 CCTCCAGAAGG EcoT14I 110 CCAAGG C^C[AT][AT]GG 665 CCAAGG HapII 126 CCGG C^CGG 210 CCGG HpaII 126 CCGG C^CGG 210 CCGG Ksp632I 574 CTCTTC CTCTTC(1/4) 283 GAAGAG MslI 216 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 526 CACTGCTGTG MspA1I 88 CAGCTG C[AC]G^C[GT]G 564 CCGCTG MspI 126 CCGG C^CGG 210 CCGG NciI 125 CCCGG CC^[GC]GG 210 CCGGG NspI 54 GCATGT [GA]CATG^[CT] 581 GCATGC PstI 625 CTGCAG CTGCA^G 709 CTGCAG SmlI 187 CTTGAG C^T[CT][GA]AG 202 CTCAAG StyI 110 CCAAGG C^C[AT][AT]GG 665 CCAAGG TaqI 153 TCGA T^CGA 638 TCGA TthHB8I 153 TCGA T^CGA 638 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII ItaI MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI