(Circular) (Six-base) MAPSORT of: pFN21AE6602.seq Check: 9860 from: 1 to: 5419 pFN21AE6602 5419 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3229 278 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2762 1937 Size: 825 4594 Acc65I G'GTAC_C Cuts at: 2740 2740 Size: 5419 AclI AA'CG_TT Cuts at: 1563 2435 3546 3919 1563 Size: 872 1111 373 3063 Fragments arranged by size: 3063 1111 872 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2566 2726 3482 4866 5134 1632 Size: 934 160 756 1384 268 1917 Fragments arranged by size: 1917 1384 934 756 268 160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5222 AflIII A'CryG_T Cuts at: 1203 4318 5295 1203 Size: 3115 977 1327 Fragments arranged by size: 3115 1327 977 AgeI A'CCGG_T Cuts at: 4250 4250 Size: 5419 AhdI GACnn_n'nnGTC Cuts at: 4148 4148 Size: 5419 AleI CACnn'nnGTG Cuts at: 1166 2535 1166 Size: 1369 4050 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5419 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 5387 AlwNI CAG_nnn'CTG Cuts at: 2095 2098 3162 4734 2095 Size: 3 1064 1572 2780 Fragments arranged by size: 2780 1572 1064 3 ApaI G_GGCC'C Cuts at: 2131 2578 2131 Size: 447 4972 ApaLI G'TGCA_C Cuts at: 3476 4632 3476 Size: 1156 4263 ApoI r'AATT_y Cuts at: 1493 2728 2954 3106 5215 1493 Size: 1235 226 152 2109 1697 Fragments arranged by size: 2109 1697 1235 226 152 AseI AT'TA_AT Cuts at: 160 1032 3973 160 Size: 872 2941 1606 Fragments arranged by size: 2941 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 AvaI C'yCGr_G Cuts at: 1870 1943 2744 1870 Size: 73 801 4545 Fragments arranged by size: 4545 801 73 BamHI G'GATC_C Cuts at: 2749 3116 2749 Size: 367 5052 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2379 2615 2740 4195 618 Size: 325 217 627 592 236 125 1455 1842 Fragments arranged by size: 1842 1455 627 592 325 236 217 125 BanII G_rGCy'C Cuts at: 1391 2109 2131 2578 2738 1391 Size: 718 22 447 160 4072 Fragments arranged by size: 4072 718 447 160 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4448 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3610 3644 1903 Size: 34 1673 34 3678 Fragments arranged by size: 3678 1673 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2308 3311 4521 2308 Size: 1003 1210 3206 Fragments arranged by size: 3206 1210 1003 BclI T'GATC_A Cuts at: 1548 1548 Size: 5419 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 4030 136 Size: 107 122 71 631 605 454 1904 1525 Fragments arranged by size: 1904 1525 631 605 454 122 107 71 BglII A'GATC_T Cuts at: 5414 5414 Size: 5419 BlpI GC'TnA_GC Cuts at: 2836 2836 Size: 5419 Bme1580I G_kGCm'C Cuts at: 1790 2131 2578 3480 4636 1790 Size: 341 447 902 1156 2573 Fragments arranged by size: 2573 1156 902 447 341 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3150 4108 442 Size: 416 53 83 399 24 1733 958 1753 Fragments arranged by size: 1753 1733 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5419 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2279 2318 4079 1239 Size: 799 241 39 1761 2579 Fragments arranged by size: 2579 1761 799 241 39 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 5419 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2192 2213 3544 4409 4707 2192 Size: 21 1331 865 298 2904 Fragments arranged by size: 2904 1331 865 298 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4082 882 Size: 617 2583 2219 Fragments arranged by size: 2583 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2605 3210 493 Size: 711 1401 605 2702 Fragments arranged by size: 2702 1401 711 605 BsaBI GATnn'nnATC Cuts at: 5413 5413 Size: 5419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3226 3608 4243 275 Size: 53 83 186 936 1693 382 635 1451 Fragments arranged by size: 1693 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3852 4250 4524 4671 5082 3852 Size: 398 274 147 411 4189 Fragments arranged by size: 4189 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5194 Fragments arranged by size: 5194 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2195 2630 1186 Size: 1006 3 435 3975 Fragments arranged by size: 3975 1006 435 3 BseYI C'CCAG_C Cuts at: 2088 2108 2571 4622 5118 2088 Size: 20 463 2051 496 2389 Fragments arranged by size: 2389 2051 496 463 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2513 1163 Size: 573 777 4069 Fragments arranged by size: 4069 777 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2869 3630 4658 1540 Size: 275 186 868 761 1028 2301 Fragments arranged by size: 2301 1028 868 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3480 3565 4636 3480 Size: 85 1071 4263 Fragments arranged by size: 4263 1071 85 BsmI GAATG_Cn' Cuts at: 2930 3023 2930 Size: 93 5326 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2131 2578 2738 3480 3565 4636 Size: 399 319 22 447 160 742 85 1071 Cuts at: 4636 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 447 399 319 160 85 22 BspEI T'CCGG_A Cuts at: 5082 5082 Size: 5419 BspHI T'CATG_A Cuts at: 2553 3308 5038 2553 Size: 755 1730 2934 Fragments arranged by size: 2934 1730 755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2637 2707 2774 844 Size: 1793 70 67 3489 Fragments arranged by size: 3489 1793 70 67 BsrBI CCG'CTC Cuts at: 1394 1823 3306 1394 Size: 429 1483 3507 Fragments arranged by size: 3507 1483 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3914 4088 65 Size: 1183 2666 174 1396 Fragments arranged by size: 2666 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4063 4250 1700 Size: 110 2253 187 2869 Fragments arranged by size: 2869 2253 187 110 BsrGI T'GTAC_A Cuts at: 96 2445 96 Size: 2349 3070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5419 BssSI C'ACGA_G Cuts at: 3479 4491 3479 Size: 1012 4407 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5419 BstBI TT'CG_AA Cuts at: 4223 4223 Size: 5419 BstEII G'GTnAC_C Cuts at: 1184 2083 1184 Size: 899 4520 BstYI r'GATC_y Cuts at: 1972 2749 3116 3501 3518 4970 5056 5068 5414 Size: 777 367 385 17 1452 86 12 346 Cuts at: 5414 1972 Size: 1977 Fragments arranged by size: 1977 1452 777 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3928 Fragments arranged by size: 3928 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4680 Fragments arranged by size: 4680 683 56 BtsI GCAGTG_nn' Cuts at: 2530 2935 3729 3749 2530 Size: 405 794 20 4200 Fragments arranged by size: 4200 794 405 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5384 DraI TTT'AAA Cuts at: 2723 2723 Size: 5419 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4426 809 Size: 507 3110 1802 Fragments arranged by size: 3110 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2866 3755 5384 Size: 54 1425 50 275 18 1036 889 1629 Cuts at: 5384 8 Size: 43 Fragments arranged by size: 1629 1425 1036 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2866 1537 Size: 275 1054 4090 Fragments arranged by size: 4090 1054 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 2585 3349 1353 Size: 930 302 764 3423 Fragments arranged by size: 3423 930 764 302 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 4006 4380 4526 387 Size: 1064 138 579 1838 374 146 1280 Fragments arranged by size: 1838 1280 1064 579 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2279 2318 2566 2726 3482 4079 Size: 393 406 241 39 248 160 756 597 Cuts at: 4079 4866 5134 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 756 597 406 393 268 248 241 160 39 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2336 2207 Size: 129 5290 EcoO109I rG'GnC_Cy Cuts at: 2119 2127 2197 2575 2119 Size: 8 70 378 4963 Fragments arranged by size: 4963 378 70 8 EcoRI G'AATT_C Cuts at: 2728 3106 2728 Size: 378 5041 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 5419 FspI TGC'GCA Cuts at: 3925 3925 Size: 5419 HaeII r_GCGC'y Cuts at: 1678 4566 1678 Size: 2888 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 4060 4092 4134 Size: 32 135 32 923 32 1533 32 42 Cuts at: 4134 4166 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 923 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2763 3009 669 Size: 988 281 825 246 3079 Fragments arranged by size: 3079 988 825 281 246 HindIII A'AGCT_T Cuts at: 748 2562 748 Size: 1814 3605 HpaI GTT'AAC Cuts at: 3009 3009 Size: 5419 KpnI G_GTAC'C Cuts at: 2744 2744 Size: 5419 MfeI C'AATT_G Cuts at: 3018 3018 Size: 5419 MluI A'CGCG_T Cuts at: 5295 5295 Size: 5419 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 4533 4717 1305 Size: 777 2451 184 2007 Fragments arranged by size: 2451 2007 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3597 Fragments arranged by size: 3597 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2535 3377 3736 3895 518 Size: 648 1059 310 842 359 159 2042 Fragments arranged by size: 2042 1059 842 648 359 310 159 MspA1I CmG'CkG Cuts at: 1606 2095 2571 2835 3512 4660 4905 1606 Size: 489 476 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 476 264 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3928 Fragments arranged by size: 3928 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5419 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5419 NotI GC'GGCC_GC Cuts at: 2866 2866 Size: 5419 NspI r_CATG'y Cuts at: 1132 1229 2063 2590 2777 4322 1132 Size: 97 834 527 187 1545 2229 Fragments arranged by size: 2229 1545 834 527 187 97 PasI CC'CwG_GG Cuts at: 1348 2124 1348 Size: 776 4643 PciI A'CATG_T Cuts at: 4318 4318 Size: 5419 PflMI CCAn_nnn'nTGG Cuts at: 1108 2305 1108 Size: 1197 4222 PfoI T'CCnGG_A Cuts at: 2214 2214 Size: 5419 PmeI GTTT'AAAC Cuts at: 2723 2723 Size: 5419 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3488 3520 5001 5033 3488 Size: 32 1481 32 3874 Fragments arranged by size: 3874 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2197 Size: 5419 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5419 PsiI TTA'TAA Cuts at: 2989 2989 Size: 5419 PspOMI G'GGCC_C Cuts at: 2127 2574 2127 Size: 447 4972 PstI C_TGCA'G Cuts at: 830 2634 2718 2771 830 Size: 1804 84 53 3478 Fragments arranged by size: 3478 1804 84 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5419 PvuII CAG'CTG Cuts at: 2095 2095 Size: 5419 SalI G'TCGA_C Cuts at: 1936 2761 1936 Size: 825 4594 SbfI CC_TGCA'GG Cuts at: 2771 2771 Size: 5419 ScaI AGT'ACT Cuts at: 2294 3667 5379 2294 Size: 1373 1712 2334 Fragments arranged by size: 2334 1712 1373 SfcI C'TryA_G Cuts at: 826 1045 2277 2630 2714 2767 3902 4583 4774 Size: 219 1232 353 84 53 1135 681 191 Cuts at: 4774 826 Size: 1471 Fragments arranged by size: 1471 1232 1135 681 353 219 191 84 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2126 2126 Size: 5419 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5419 SmaI CCC'GGG Cuts at: 1872 2746 1872 Size: 874 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 2207 3523 4424 4686 820 Size: 197 926 249 15 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 262 249 197 15 SnaBI TAC'GTA Cuts at: 493 3210 493 Size: 2717 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5419 SphI G_CATG'C Cuts at: 1132 2590 2777 1132 Size: 1458 187 3774 Fragments arranged by size: 3774 1458 187 SspI AAT'ATT Cuts at: 5 52 3343 5 Size: 47 3291 2081 Fragments arranged by size: 3291 2081 47 StuI AGG'CCT Cuts at: 1517 2681 1517 Size: 1164 4255 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2670 2858 513 Size: 552 939 111 555 188 3074 Fragments arranged by size: 3074 939 555 552 188 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2397 3446 3463 3616 3801 4246 325 Size: 1255 817 1049 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1049 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 2445 3665 Size: 274 80 33 51 1443 315 153 1220 Cuts at: 3665 5377 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1220 315 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 5379 5411 2372 Size: 32 2975 32 2380 Fragments arranged by size: 2975 2380 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5419 XbaI T'CTAG_A Cuts at: 2755 2755 Size: 5419 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2609 2609 Size: 5419 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5419 XmaI C'CCGG_G Cuts at: 1870 2744 1870 Size: 874 4545 XmnI GAAnn'nnTTC Cuts at: 3548 3548 Size: 5419 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3227 276 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI